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文献和实验Primary Culture and Differentiation of Human Adipocyte Precursor Cells
number and, owing to their enormous cell size, make up more than 90% of the tissue volume. Adipocytes are major participants in the energy homeostasis of the human body by storing excess energy in the form of triglycerides and by releasing fatty acids
Isolation and Culture of Primary Human CNS Neural Cells
We present our current methods for isolating and culturing cells from the adult and fetal human CNS. The cell isolation procedures used are also well suited to obtain samples for immediate RNA- and protein-based analyses, particularly
Immunohistochemistry: Mouse Anti-Human MCP-1
antigen retrieval with 1mM EDTA pH 8.0 at 123?C for 3 minutes in a Pascal pressure chamber (Dako). Wash the slide twice for three minutes each (50mM Tris-HCl pH 8.0, 150mM NaCl in distilled water). 3. Incubate the tissue section with 1% BSA/PBS block
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