免疫球蛋白G1酶联免疫试剂盒(小鼠) IgG1 ELISA

产品概述

免疫球蛋白G1酶联免疫试剂盒（小鼠）（Mouse IgG1 ELISA Kit，货号 KTE3022-3）是一款专为 细胞培养上清、血清及血浆 中小鼠 IgG1 定量设计的核心ELISA检测试剂盒，采用EliKine™ 双抗体夹心法，实现高灵敏度、低背景的检测。

关键应用领域

• 蛋白类分析 — 适用于小鼠来源的IgG1抗体水平测定。
• ELISA平台 — 兼容常规酶标仪（450 nm），支持高通量96孔或48孔板实验。
• 细胞分析中心 — 可用于细胞培养上清、血清、血浆等样本的免疫学研究。

检测原理

本套件基于 双抗体夹心ELISA，工作流程如下：

1. 固定在微孔板上的特异性抗小鼠 IgG1 捕获抗体在孔底形成固相。
2. 加入待测样本或标准品，IgG1 与捕获抗体形成第一层复合物。
3. 加入 HRP 标记的检测抗体（抗小鼠 IgG1），形成第二层复合物。
4. 洗涤去除未结合组分，随后加入 TMB 显色底物，HRP 催化产生蓝色信号。
5. 加入终止液，颜色转为黄色，在 450 nm 读取光密度（OD），OD 与样本中 IgG1 含量呈正比。

试剂盒组成

• Mouse IgG1 Microplate（预涂层96/48孔）
• Mouse IgG1 Standard (lyophilized)
• Assay Buffer (10×)
• HRP‑conjugated Mouse IgG1 Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

性能参数

检测范围	3.13 ng/mL – 200 ng/mL
灵敏度（LOD）	3.13 ng/mL
交叉反应/背景	未见显著交叉反应或背景干扰（经EliKine™验证）

使用注意事项

• 不同批号或超过有效期的试剂不得混用。
• 试剂使用前请于室温平衡 30 分钟。
• 操作时建议使用一次性吸头，防止交叉污染。
• 未使用的孔板请立即放回包装，密封后在 4 ℃保存。
• 混匀步骤对结果影响显著，推荐使用低速振荡器或每10 分钟手动摇匀。
• 样品和标准品建议采用双孔或三孔复孔检测，以提高数据可靠性。

储存与运输

• 未开封时：避光保存于 2‑8 ℃，有效期 24 个月。
• 启用后：请参照随盒说明书对各组分分别存放。
• 运输条件：使用蓝冰（冰袋）冷链运输，确保全程温度控制。

安全与合规

警告：本产品仅供科研使用，不适用于人类或临床诊断。请勿将本套件用于任何侵权或违反专利、许可证的活动，因不当使用导致的责任概不负责。

技术背景

IgG 是血浆 B 细胞分泌的主要抗体，在体液免疫防御中发挥关键作用。小鼠 IgG1 与人类 IgG1 在功能上有一定相似性，常用于免疫学模型和自身免疫性疾病研究。定量检测小鼠 IgG1 可提供免疫状态、疫苗反应或疾病进展的直观指标。

订购信息

包装规格	单价（人民币）	交货期
48 T	1,598.00	期货（5 天内发货）
96 T	2,558.00	期货（5 天内发货）

关键字检索

IgG1, 小鼠 IgG1, ELISA Kit, 免疫球蛋白 G1, 细胞分析, 蛋白定量, 双抗体夹心法

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Immunoglobulin G1 Enzyme-Linked Immunosorbent Assay Kit (Mouse) (Mouse IgG1 ELISA Kit, Catalog Number KTE3022-3) is a core ELISA detection kit specifically designed for the quantitative measurement of mouse IgG1 in cell culture supernatants, serum, and plasma. Utilizing the EliKine™ double-antibody sandwich method, it achieves high sensitivity and low background detection.

Key Application Areas

• Protein Analysis — Suitable for measuring IgG1 antibody levels from mouse sources.
• ELISA Platform — Compatible with standard microplate readers (450 nm), supporting high-throughput 96-well or 48-well plate experiments.
• Cell Analysis Center — Can be used for immunological studies on samples such as cell culture supernatants, serum, and plasma.

Detection Principle

This kit is based on the double-antibody sandwich ELISA principle. The workflow is as follows:

1. A specific anti-mouse IgG1 capture antibody is immobilized on the microplate well bottom, forming a solid phase.
2. Add the test sample or standard; IgG1 binds to the capture antibody, forming the first complex.
3. Add the HRP-conjugated detection antibody (anti-mouse IgG1), forming the second complex.
4. Wash away unbound components, then add the TMB chromogenic substrate. HRP catalyzes the reaction to produce a blue signal.
5. Add the stop solution, turning the color yellow. Measure the optical density (OD) at 450 nm, which is proportional to the IgG1 concentration in the sample.

Kit Components

• Mouse IgG1 Microplate (pre-coated, 96/48 wells)
• Mouse IgG1 Standard (lyophilized)
• Assay Buffer (10×)
• HRP‑conjugated Mouse IgG1 Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Performance Parameters

Detection Range	3.13 ng/mL – 200 ng/mL
Sensitivity (LOD)	3.13 ng/mL
Cross-Reactivity/Background	No significant cross-reactivity or background interference observed (verified by EliKine™)

Usage Notes

• Do not mix reagents from different lots or those beyond the expiration date.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips to prevent cross-contamination during operation.
• Immediately return unused wells to the original packaging, seal, and store at 4 ℃.
• Thorough mixing significantly affects results; use a low-speed shaker or manually shake every 10 minutes.
• It is recommended to test samples and standards in duplicate or triplicate wells to improve data reliability.

Storage and Shipping

• Unopened: Store protected from light at 2‑8 ℃. Shelf life is 24 months.
• After Opening: Refer to the included instructions for specific storage conditions of each component.
• Shipping Conditions: Shipped with blue ice (ice packs) via cold chain to ensure temperature control throughout transit.

Safety and Compliance

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Do not use this kit for any activities that infringe upon or violate patents or licenses. No liability is assumed for improper use.

Technical Background

IgG is the primary antibody secreted by plasma B cells and plays a key role in humoral immune defense. Mouse IgG1 shares certain functional similarities with human IgG1 and is commonly used in immunological models and autoimmune disease research. Quantitative detection of mouse IgG1 provides a direct indicator of immune status, vaccine response, or disease progression.

Ordering Information

Package Size	Unit Price (RMB)	Delivery Time
48 T	1,598.00	Future Goods (Ships within 5 days)
96 T	2,558.00	Future Goods (Ships within 5 days)

Keyword Search

IgG1, Mouse IgG1, ELISA Kit, Immunoglobulin G1, Cell Analysis, Protein Quantification, Double-Antibody Sandwich Method

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.