小鼠B细胞活化因子 ELISA定量试剂盒(BAFF/BLyS

产品概述

EliKine™ 小鼠B细胞活化因子(BAFF/BLyS/TNFSF13B) ELISA定量试剂盒（货号：KTE3096-1）是一款专为精准定量小鼠样本中BAFF蛋白浓度而设计的高性能检测工具。本试剂盒采用成熟的夹心法ELISA原理，适用于细胞培养上清、血清及血浆等多种样本类型，检测范围宽广（46.88 pg/mL - 3,000 pg/mL），具备出色的灵敏度（46.88 pg/mL），可满足您在B细胞免疫研究领域的高标准需求。

核心优势与特性

• 高特异性与低背景干扰：经严格验证，对目标蛋白的类似物无明显交叉反应，确保检测结果的准确性与可靠性。
• 优化的检测性能：宽广的线性范围与高灵敏度，便于精确检测低丰度样本，支持可靠的定量分析。
• 便捷的即用型设计：试剂盒提供完整组分，包括预包被微孔板、冻干标准品、检测抗体及显色底物等，实验流程清晰，操作简便。
• 严格的质控标准：每个批号均经过严格质量控制，确保批次间稳定性。

应用领域

本试剂盒主要用于B细胞相关免疫学研究，特别适用于：

• 自身免疫性疾病机制研究（如系统性红斑狼疮、类风湿性关节炎）
• B细胞增殖、分化及功能评估
• 免疫调节剂或药物疗效的体外评估

试剂盒组分

组分名称	规格
Mouse BAFF Microplate	1块
Mouse BAFF Standard (lyophilized)	2支
Standard Diluent	1瓶
Assay Buffer (10×)	1瓶
Mouse BAFF Detect Antibody (100×)	1瓶
Streptavidin-HRP (100×)	1瓶
HRP Substrate (TMB)	1瓶
Stop Solution	1瓶
Wash Buffer (20×)	1瓶
Plate Covers	3片

技术参数

• 产品类型：ELISA定量试剂盒
• 反应种属：小鼠
• 样本类型：细胞培养上清、血清、血浆
• 检测方法：夹心法ELISA
• 检测范围：46.88 pg/mL - 3,000 pg/mL
• 灵敏度：46.88 pg/mL
• 规格选择：48 T（货期请咨询） / 96 T（期货，5天内发货）

保存与运输

• 保存建议：未开封试剂盒避光保存于2-8℃，保质期24个月；启用后请参照说明书保存各组分。
• 运输条件：冰袋运输（蓝冰）

注意事项

• 请勿混合使用不同批号或过期试剂。
• 使用前请室温平衡30分钟，并按标签说明储存。
• 实验过程中建议使用一次性吸头，避免交叉污染。
• 推荐使用复孔检测（双孔或三孔）以提高数据可靠性。
• 未使用的板条请立即密封，4℃保存。

背景信息

B细胞活化因子（BAFF，亦称BLyS或TNFSF13B）是肿瘤坏死因子超家族成员，主要表达于B细胞系，作为关键的B细胞激活剂，在B细胞增殖、分化及抗体产生中发挥核心作用。BAFF表达水平异常与多种免疫疾病密切相关，其表达不足可能导致免疫缺陷，而过量表达则与系统性红斑狼疮、类风湿性关节炎等自身免疫疾病的发生发展有关。

警告：本产品仅供研究使用，不适用于人类或临床诊断。使用前请查阅相关文献并确保符合当地法规要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The EliKine™ Mouse B Cell Activating Factor (BAFF/BLyS/TNFSF13B) ELISA Quantitation Kit (Cat. #: KTE3096-1) is a high-performance assay tool specifically designed for the accurate quantification of mouse BAFF protein concentration in samples. This kit employs the well-established sandwich ELISA principle and is suitable for various sample types including cell culture supernatants, serum, and plasma. It features a broad detection range (46.88 pg/mL - 3,000 pg/mL) and excellent sensitivity (46.88 pg/mL), meeting your high-standard requirements in B cell immunology research.

Key Advantages & Features

• High Specificity & Low Background Interference: Rigorously validated to show no significant cross-reactivity with analogs of the target protein, ensuring accurate and reliable results.
• Optimized Assay Performance: Broad linear range and high sensitivity facilitate precise detection of low-abundance samples, supporting reliable quantitation.
• Convenient Ready-to-Use Design: The kit provides complete components, including pre-coated microplate, lyophilized standard, detection antibody, chromogenic substrate, etc., with a clear protocol for straightforward operation.
• Stringent Quality Control Standards: Each lot undergoes rigorous quality control to ensure inter-batch consistency.

Applications

This kit is primarily used for B cell-related immunology research, particularly suitable for:

• Mechanistic studies of autoimmune diseases (e.g., Systemic Lupus Erythematosus, Rheumatoid Arthritis)
• Assessment of B cell proliferation, differentiation, and function
• In vitro evaluation of immunomodulator or drug efficacy

Kit Components

Component Name	Specification
Mouse BAFF Microplate	1 plate
Mouse BAFF Standard (lyophilized)	2 vials
Standard Diluent	1 bottle
Assay Buffer (10×)	1 bottle
Mouse BAFF Detect Antibody (100×)	1 bottle
Streptavidin-HRP (100×)	1 bottle
HRP Substrate (TMB)	1 bottle
Stop Solution	1 bottle
Wash Buffer (20×)	1 bottle
Plate Covers	3 sheets

Technical Parameters

• Product Type: ELISA Quantitation Kit
• Reactivity: Mouse
• Sample Type: Cell culture supernatant, Serum, Plasma
• Assay Method: Sandwich ELISA
• Detection Range: 46.88 pg/mL - 3,000 pg/mL
• Sensitivity: 46.88 pg/mL
• Size Options: 48 T (Lead time inquiry) / 96 T (Future product, ships within 5 days)

Storage & Shipping

• Storage Recommendation: Store the unopened kit protected from light at 2-8°C; shelf life is 24 months. After opening, store components as instructed in the manual.
• Shipping Conditions: Shipped with ice packs (blue ice).

Precautions

• Do not mix reagents from different lots or use expired reagents.
• Allow all components to equilibrate to room temperature for 30 minutes before use and store as indicated on the labels.
• Use disposable pipette tips during the assay to avoid cross-contamination.
• Replicate testing (duplicate or triplicate wells) is recommended to improve data reliability.
• Immediately reseal any unused strips and store at 4°C.

Background Information

B Cell Activating Factor (BAFF, also known as BLyS or TNFSF13B) is a member of the tumor necrosis factor (TNF) superfamily, primarily expressed in cells of the B-lineage. As a key B cell activator, it plays a central role in B cell proliferation, differentiation, and antibody production. Aberrant BAFF expression levels are closely associated with various immune disorders; insufficient expression may lead to immunodeficiency, while overexpression is implicated in the pathogenesis of autoimmune diseases such as Systemic Lupus Erythematosus and Rheumatoid Arthritis.

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. Please review relevant literature and ensure compliance with local regulations before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please contact YaKeyin Company.