IgG1 ELISA KIT 免疫球蛋白G1ELISA试剂盒

EliKine™ 小鼠免疫球蛋白G1 (IgG1) ELISA试剂盒 (KTE3022-2)

本试剂盒是一款基于经典双抗体夹心法原理开发的高灵敏度、高特异性定量检测工具，专门用于精准检测小鼠血清、血浆及细胞培养上清样本中的免疫球蛋白G1 (IgG1)含量。作为细胞分析领域的核心产品，它经过优化设计，为生命科学研究提供可靠的数据支持。

产品核心特性

• 高灵敏度与宽检测范围：最低检测浓度可达3.13 ng/mL，检测范围为3.13 ng/mL - 200 ng/mL，确保对低丰度样本的准确定量和广泛样本浓度的覆盖。
• 卓越的特异性：经严格验证，对小鼠IgG1的类似物检测未观察到明显的交叉反应或背景干扰，实验结果准确可靠。
• 即用型完整组分：试剂盒提供包括预包被微孔板、冻干标准品、检测抗体、底物等在内的全套优质组分，开盒即用，简化实验流程。
• 优化的实验方案：详细的说明书提供了经过验证的操作步骤，有助于研究人员获得稳定、可重复的实验结果。

技术参数详情

参数	详情
产品货号	KTE3022-2
反应种属	小鼠
检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆
保存条件	未开封试剂盒请避光保存于2-8℃，有效期24个月。启用后请参照说明书妥善保存各组分。
运输条件	冰袋（蓝冰）运输，确保试剂活性。

试剂盒组分

• 小鼠IgG1预包被微孔板 (Mouse IgG1 Microplate)
• 小鼠IgG1标准品（冻干） (Mouse IgG1 Standard, lyophilized)
• 测定缓冲液 (10×) (Assay Buffer 10×)
• HRP标记的小鼠IgG1检测抗体 (100×) (HRP-conjugated Mouse IgG1 Detect Antibody 100×)
• HRP底物 (TMB) (HRP Substrate TMB)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×) (Wash Buffer 20×)
• 板盖 (Plate Covers)

（注：具体组分数量请参考随货说明书。）

实验流程简介

1. 将样本/标准品与HRP标记检测抗体加入预包被孔中温育。
2. 洗涤去除未结合物质。
3. 加入TMB底物显色，HRP催化反应产生蓝色产物。
4. 加入终止液，溶液变为黄色。
5. 于450nm波长下检测吸光度(OD值)，IgG1浓度与OD值成正比。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前所有试剂应室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条需立即放回原包装袋并密封，于4℃保存。
• 充分混匀试剂与样本对结果至关重要，建议使用振荡器或定期手动摇匀。
• 为获得更精确的结果，建议对样品和标准品进行复孔检测（双孔或三孔）。

订购信息

规格	价格 (人民币)	货期
48 T	1598.0	期货（预计5个工作日内发货）
96 T	2558.0	期货（预计5个工作日内发货）

背景知识

免疫球蛋白G (IgG) 是体液免疫应答中的关键效应分子，由浆细胞分泌。在小鼠中，IgG1是重要的抗体亚类之一，参与调节免疫反应。定量检测小鼠IgG1水平对于评估免疫状态、研究感染性疾病模型、自身免疫疾病以及抗体药物研发等领域具有重要价值。

警告：本产品仅限用于科学研究，不得用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Immunoglobulin G1 (IgG1) ELISA Kit (KTE3022-2)

This kit is a high-sensitivity, high-specificity quantitative detection tool developed based on the classic double-antibody sandwich principle, specifically designed for the accurate detection of Immunoglobulin G1 (IgG1) levels in mouse serum, plasma, and cell culture supernatant samples. As a core product in the field of cell analysis, it is optimally designed to provide reliable data support for life science research.

Core Features

• High Sensitivity and Broad Detection Range: The minimum detectable concentration can reach 3.13 ng/mL, with a detection range of 3.13 ng/mL - 200 ng/mL, ensuring accurate quantification of low-abundance samples and coverage of a wide range of sample concentrations.
• Excellent Specificity: Rigorously validated, no significant cross-reactivity or background interference was observed with analogs of mouse IgG1, ensuring accurate and reliable experimental results.
• Ready-to-Use Complete Components: The kit provides a full set of high-quality components, including pre-coated microplate, lyophilized standard, detection antibody, substrate, etc., ready for immediate use, simplifying the experimental procedure.
• Optimized Protocol: The detailed manual provides validated operating steps, aiding researchers in obtaining stable and reproducible experimental results.

Technical Parameters

Parameter	Details
Catalog Number	KTE3022-2
Reactivity Species	Mouse
Detection Method	Sandwich ELISA
Sample Type	Cell Culture Supernatant, Serum, Plasma
Storage Conditions	Store the unopened kit protected from light at 2-8°C; valid for 24 months. After opening, store components properly according to the manual.
Shipping Conditions	Shipped with ice packs (blue ice) to ensure reagent activity.

Kit Components

• Mouse IgG1 Pre-coated Microplate (Mouse IgG1 Microplate)
• Mouse IgG1 Standard (lyophilized) (Mouse IgG1 Standard, lyophilized)
• Assay Buffer (10×) (Assay Buffer 10×)
• HRP-conjugated Mouse IgG1 Detection Antibody (100×) (HRP-conjugated Mouse IgG1 Detect Antibody 100×)
• HRP Substrate (TMB) (HRP Substrate TMB)
• Stop Solution (Stop Solution)
• Wash Buffer (20×) (Wash Buffer 20×)
• Plate Covers (Plate Covers)

(Note: Please refer to the accompanying manual for specific component quantities.)

Brief Experimental Procedure

1. Incubate samples/standards with the HRP-conjugated detection antibody in the pre-coated wells.
2. Wash to remove unbound substances.
3. Add TMB substrate for color development; the HRP-catalyzed reaction produces a blue product.
4. Add stop solution; the solution turns yellow.
5. Measure the absorbance (OD value) at 450 nm; the IgG1 concentration is proportional to the OD value.

Important Precautions

• Do not mix reagents from different lots or expired reagents.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• Immediately return unused strips to the original pouch and seal them for storage at 4°C.
• Thorough mixing of reagents and samples is crucial for results; using a shaker or periodic manual shaking is recommended.
• For more accurate results, duplicate or triplicate wells for samples and standards are recommended.

Ordering Information

Specification	Price (RMB)	Lead Time
48 T	1598.0	Future goods (estimated shipment within 5 business days)
96 T	2558.0	Future goods (estimated shipment within 5 business days)

Background

Immunoglobulin G (IgG) is a key effector molecule in the humoral immune response, secreted by plasma cells. In mice, IgG1 is one of the important antibody subclasses involved in regulating immune responses. Quantitative detection of mouse IgG1 levels is valuable for assessing immune status, studying infectious disease models, autoimmune diseases, and antibody drug development.

Warning: This product is intended for research use only, not for human or clinical diagnostic use. Users should ensure their application does not violate relevant patent or licensing regulations.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yaken Company for specific details.