小鼠视黄醇结合蛋白4 ELISA定量试剂盒(PRBP/RBP

小鼠视黄醇结合蛋白4(RBP4) ELISA定量试剂盒(一步法)

货号: KTE4069-1

产品类型: EliKine™ ELISA核心试剂盒

反应性: 小鼠

检测样本类型: 血清、血浆、组织匀浆液、细胞培养上清液及其他相关生物液体样本

产品概述

本试剂盒采用优化的双抗体夹心一步法ELISA原理，专门用于精准定量检测小鼠来源样本中的视黄醇结合蛋白4（RBP4）含量。RBP4是一种主要由肝脏合成的重要转运蛋白，负责视黄醇（维生素A）在血液中的运输，其水平变化与代谢调节、肾脏功能及胰岛素敏感性等生理病理过程密切相关。本试剂盒经过严格验证，为研究者提供稳定、可靠的检测工具。

主要特点与优势

• 高灵敏度：最低检测浓度可达0.05 ng/mL，能够准确测量样本中的低丰度RBP4。
• 优异特异性：经过广泛测试，与小鼠RBP4的类似物无明显交叉反应，背景信号低，确保结果准确性。
• 宽线性范围：检测范围为0.25 ng/mL - 20 ng/mL，涵盖生理及病理状态下的大部分浓度。
• 操作便捷：一步法检测流程，简化了实验步骤，节省操作时间。
• 试剂盒组分稳定：所有组分均经过严格质控，批间差小，保证实验结果的可重复性。

检测原理

本试剂盒采用双抗体夹心酶联免疫吸附测定法。预包被于微孔板上的特异性抗体捕获样本中的RBP4抗原，随后依次与生物素标记的检测抗体和链霉亲和素-HRP结合，形成免疫复合物。加入TMB底物显色后，颜色的深浅与样本中RBP4的浓度成正比。通过测定450 nm处的吸光度值，并参照标准曲线即可计算出RBP4的精确浓度。

试剂盒组分

• 预包被小鼠RBP4微孔板 (Mouse RBP4 Microplate)
• 小鼠RBP4标准品 (40 ng/mL) (Mouse RBP4 Standard)
• 标准品稀释液 (Standard Diluent)
• 生物素标记的小鼠RBP4检测抗体 (Mouse RBP4 Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A液 (HRP Substrate A)
• HRP底物B液 (HRP Substrate B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (浓缩液) (Wash Buffer)
• 板盖 (Plate Covers)

注意：详细组分数量请参考试剂盒说明书。

规格与价格

规格	价格 (人民币)	货期
48 T (可检测42个样本/21个标准品复孔)	1,998.0	期货（预计3个工作日内发货）
96 T (可检测90个样本/45个标准品复孔)	3,198.0	期货（预计3个工作日内发货）

保存与运输

• 保存：未开封的试剂盒请避光保存于2-8℃，保质期为6个月。启用后，请根据说明书指示分别保存各组分。
• 运输：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂盒组分。
• 使用前，所有试剂应在室温平衡约30分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 暂时不用的板条应放回原包装袋并密封，于4℃保存。
• 为确保反应充分，建议在孵育过程中使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对标准品和样品进行双孔或三孔重复检测。

背景信息

视黄醇结合蛋白4（RBP4），也被称为血浆视黄醇结合蛋白（PRBP或RBP），是维生素A（视黄醇）的主要血液转运蛋白。近年来，研究发现RBP4与胰岛素抵抗、2型糖尿病、肥胖及某些肾脏疾病的发病机制有关，使其成为代谢研究领域的一个重要生物标志物。

警告： 本产品仅用于科学研究，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Retinol Binding Protein 4 (RBP4) ELISA Quantitation Kit (One-Step)

Item No.: KTE4069-1

Product Type: EliKine™ ELISA Core Kit

Species Reactivity: Mouse

Sample Types: Serum, plasma, tissue homogenate, cell culture supernatant and other related biological fluid samples

Product Overview

This kit employs an optimized double‑antibody sandwich one‑step ELISA principle, specifically designed for the precise quantitative detection of Retinol Binding Protein 4 (RBP4) in mouse‑derived samples. RBP4 is a major transport protein synthesized primarily in the liver, responsible for the circulation of retinol (vitamin A). Its level fluctuations are closely associated with metabolic regulation, renal function, and insulin sensitivity. The kit has been rigorously validated to provide researchers with a stable and reliable analytical tool.

Key Features and Advantages

• High Sensitivity: Lowest detection limit reaches 0.05 ng/mL, enabling accurate measurement of low‑abundance RBP4.
• Excellent Specificity: Extensively tested with no significant cross‑reactivity to mouse RBP4 homologs; low background signal ensures result accuracy.
• Wide Linear Range: Quantification range of 0.25 ng/mL – 20 ng/mL, covering most physiological and pathological concentrations.
• Convenient Workflow: One‑step assay simplifies procedures and saves hands‑on time.
• Stable Reagent Components: All components undergo strict quality control with minimal inter‑batch variation, guaranteeing reproducibility.

Assay Principle

The kit utilizes a double‑antibody sandwich enzyme‑linked immunosorbent assay. A capture antibody pre‑coated on the microplate binds RBP4 antigen present in the sample, followed sequentially by a biotin‑labeled detection antibody and streptavidin‑HRP, forming an immune complex. After addition of the TMB substrate, color development is proportional to the RBP4 concentration. Absorbance at 450 nm is measured and compared against a standard curve to calculate the exact RBP4 concentration.

Kit Components

• Pre‑coated Mouse RBP4 Microplate
• Mouse RBP4 Standard (40 ng/mL)
• Standard Diluent
• Biotin‑labeled Mouse RBP4 Detection Antibody
• Streptavidin‑HRP
• HRP Substrate Solution A
• HRP Substrate Solution B
• Stop Solution
• Wash Buffer (Concentrate)
• Plate Covers

Note: Refer to the kit manual for the exact number of each component.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T (supports 42 samples / 21 duplicate standards)	1,998.0	In stock (estimated shipment within 3 business days)
96 T (supports 90 samples / 45 duplicate standards)	3,198.0	In stock (estimated shipment within 3 business days)

Storage and Shipping

• Storage: Unopened kits should be protected from light and stored at 2–8 °C with a shelf life of 6 months. After opening, store individual components according to the instructions.
• Shipping: Entire kit is shipped on ice packs (blue ice) under refrigerated conditions to preserve reagent activity.

Critical Precautions

• Do not mix components from different lots or from expired kits.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unutilized plate strips should be returned to the original packaging, sealed, and stored at 4 °C.
• For optimal reaction, recommend gentle agitation with a low‑speed shaker or periodic manual mixing during incubations.
• To obtain reliable data, perform duplicate or triplicate measurements of standards and samples.

Background Information

Retinol Binding Protein 4 (RBP4), also referred to as plasma retinol‑binding protein (PRBP or RBP), is the primary carrier of vitamin A (retinol) in the bloodstream. Recent studies have linked RBP4 to the pathogenesis of insulin resistance, type 2 diabetes, obesity, and certain renal diseases, making it a valuable biomarker in metabolic research.

Warning: This product is intended for scientific research only and is not suitable for human or clinical diagnostic use. Users must ensure that their applications do not infringe any patents or licensing agreements. The company accepts no liability for any patent infringement or other violations arising from product use.

Note: Information is subject to change due to product upgrades or optimizations; please contact Yakein Co. for the latest details.