HA/Hyaluronan/Hyaluronate ELIS

Mouse Hyaluronic Acid (HA) ELISA Kit (One‑Step Method) – KTE4057‑2

产品概览

中文名称：HA/Hyaluronan/Hyaluronate ELISA KIT 透明质酸ELISA试剂盒（小鼠）
英文名称：Mouse Hyaluronic Acid (HA) ELISA Kit (One Step Method)
适用领域：蛋白类 → ELISA应用 → 细胞分析 → 核心产品

检测原理

本套盒基于 双抗体夹心法，使用特异性抗小鼠透明质酸（HA）抗体预包被于96孔微孔板。
1. 将样本或标准品加入孔中，HA分子与包被抗体形成第一层复合物。
2. 加入生物素化检测抗体，随后加入链霉亲和素‑HRP，形成 抗体‑抗原‑抗体‑酶 复合物。
3. 通过高效洗涤除去未结合组分后，加入TMB底物（HRP Substrate A/B），在HRP催化下产生蓝色信号。
4. 加入终止液后转为黄色，用酶标仪在 450 nm 测定OD值，OD与HA浓度呈正比。

关键特点与优势

• 极高的灵敏度（1 ng/mL）和宽检测范围（5 ng/mL–400 ng/mL）。
• 特异性强，未检测到显著的交叉反应或背景干扰。
• 一步法操作简化实验流程，适用于高通量筛选。
• 兼容血清、血浆、组织匀浆、细胞上清及其他液体样本。

试剂盒组成

每套包含以下项目：

• Mouse HA Microplate
• Mouse HA Standard (800 ng/mL)
• Standard Diluent
• Mouse HA Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

适用样本类型

血清、血浆、组织匀浆、细胞培养上清以及其他相关液体样本均可直接检测。建议对每个样本或标准进行双孔或三孔复孔，以提高数据可靠性。

实验操作要点

• 所有试剂请遵循瓶标签指示储存，使用前于室温平衡 30 分钟。
• 使用一次性吸头，防止交叉污染。
• 未使用的板条需立即放回包装、密闭并置于 4 ℃保存。
• 充分混匀对结果影响显著，推荐低速频率振荡或每10 分钟手动摇匀。
• 不同批次或超过有效期的试剂不可混用。

储存与运输

• 未开封：避光保存于 2‑8 ℃，有效期 6 个月。
• 开封后：请参照说明书对各组分分别保存。
• 运输方式：冰袋（蓝冰）冷链运输。

规格与报价

规格	包装数量	单价（人民币）	交货周期
48 T	48 试剂盒	1998.00	期货（3 天内发货）
96 T	96 试剂盒	3198.00	期货（3 天内发货）

免责声明

本产品仅供 科研实验使用，不适用于人类临床诊断或治疗。使用时请遵守相关专利和许可证规定，因使用本产品可能产生的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Hyaluronic Acid (HA) ELISA Kit (One‑Step Method) – KTE4057‑2

Product Overview

Chinese name: HA/Hyaluronan/Hyaluronate ELISA KIT Transparent Hyaluronic Acid ELISA Kit (Mouse)
English name: Mouse Hyaluronic Acid (HA) ELISA Kit (One Step Method)
Applicable field: Proteins → ELISA Applications → Cell Analysis → Core Product

Assay Principle

This kit utilizes a sandwich immunoassay format, employing a mouse-specific hyaluronic acid (HA) capture antibody pre‑coated on a 96‑well microplate.
1. Add samples or standards to the wells; HA molecules bind the capture antibody to form the first complex layer.
2. Add biotinylated detection antibody followed by Streptavidin‑HRP, generating an antibody‑antigen‑antibody‑enzyme complex.
3. After thorough washing to remove unbound components, add TMB substrate (HRP Substrate A/B); HRP catalyzes a blue signal.
4. Upon addition of stop solution the color shifts to yellow; measure the OD at 450 nm with a microplate reader. The OD is directly proportional to HA concentration.

Key Features and Advantages

• Exceptional sensitivity (1 ng/mL) and a broad detection range (5 ng/mL–400 ng/mL).
• High specificity with no significant cross‑reactivity or background interference detected.
• One‑step protocol streamlines workflow, suitable for high‑throughput screening.
• Compatible with serum, plasma, tissue homogenates, cell supernatants, and other liquid samples.

Kit Components

Each kit contains the following items:

• Mouse HA Microplate
• Mouse HA Standard (800 ng/mL)
• Standard Diluent
• Mouse HA Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

Applicable Sample Types

Serum, plasma, tissue homogenates, cell culture supernatants, and other relevant liquid samples can be assayed directly. It is recommended to run each sample or standard in duplicate or triplicate wells to enhance data reliability.

Critical Procedural Points

• Store all reagents according to the instructions on the bottle label; equilibrate at room temperature for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unused plates should be promptly returned to their packaging, sealed, and stored at 4 °C.
• Thorough mixing markedly affects results; recommend low‑speed orbital shaking or manual vortexing every 10 minutes.
• Do not mix reagents from different lots or those that have expired.

Storage and Shipping

• Unopened: Store protected from light at 2‑8 °C; shelf life 6 months.
• After opening: Refer to the manual for storage conditions of each component.
• Shipping method: Cold‑chain transport with ice packs (blue ice).

Specifications and Pricing

Specification	Package Quantity	Unit Price (CNY)	Lead Time
48 T	48 kits	1998.00	Spot (shipped within 3 days)
96 T	96 kits	3198.00	Spot (shipped within 3 days)

Disclaimer

This product is intended for research use only and is not suitable for human clinical diagnosis or therapy. Users must comply with applicable patents and license agreements; the company assumes no responsibility for any patent infringement or other violations arising from product use.

Note: The above information is subject to change due to product optimization, upgrades, etc. Please contact Yakeyin Corp. for the most current details.