小鼠透明质酸 ELISA定量试剂盒(HA/Hyalurona

小鼠透明质酸 (HA) ELISA定量试剂盒 (一步法)

货号: KTE4057-1

产品概述

EliKine™ 小鼠透明质酸（HA）ELISA定量试剂盒是一款基于成熟双抗体夹心法原理的高品质检测工具，专门设计用于精准定量小鼠血清、血浆、组织匀浆及细胞培养上清等液体样本中的透明质酸（亦称Hyaluronan或Hyaluronate）含量。本试剂盒作为细胞分析领域的核心产品，以其出色的性能为ELISA应用研究提供可靠支持。

核心特性与优势

• 高灵敏度检测：最低检测浓度可达1 ng/mL，能够有效捕捉样本中微量的HA水平变化。
• 宽线性范围：检测范围为5 ng/mL - 400 ng/mL，涵盖广泛的生理及病理浓度，满足多样本检测需求。
• 优异特异性：经过严格验证，对目标蛋白HA具有高度特异性，与类似物无明显交叉反应，确保检测结果的准确性。
• 一步法操作：优化的实验流程简化了操作步骤，有助于提升实验效率，并减少潜在的操作误差。

检测原理

本试剂盒采用双抗体夹心ELISA法。预包被于微孔板的特异性抗体捕获样本中的HA，随后依次与生物素化检测抗体及链霉亲和素-HRP结合，形成复合物。加入TMB底物显色，反应终止后，在450 nm波长下测定吸光度(OD值)，其OD值与样本中HA浓度呈正相关，从而实现准确定量。

试剂盒组分

• 预包被微孔板 (Mouse HA Microplate)
• HA标准品 (800 ng/mL)
• 标准品稀释液 (Standard Diluent)
• 检测抗体 (Mouse HA Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A/B (HRP Substrate A & B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

保存与运输

未开封试剂盒请避光保存于2-8℃，有效期为6个月。启用后，请参照说明书妥善保存各组分。本产品采用冰袋（蓝冰）运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中建议使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，推荐对样品和标准品进行复孔（双孔或三孔）检测。

警告： 本产品仅限于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，对此类潜在侵权行为概不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Hyaluronan (HA) ELISA Quantification Kit (One‑Step)

Product number: KTE4057-1

Product Overview

EliKine™ Mouse Hyaluronan (HA) ELISA Quantification Kit is a high‑quality detection tool based on a mature sandwich‑type dual‑antibody principle, specifically designed for precise quantification of hyaluronan (also known as Hyaluronan or Hyaluronate) in mouse serum, plasma, tissue homogenates, and cell‑culture supernatants. As a core product in the cellular analysis field, this kit provides reliable support for ELISA‑based research with its outstanding performance.

Key Features and Advantages

• High‑sensitivity detection: Minimum detectable concentration as low as 1 ng/mL, enabling effective capture of minute HA level changes in samples.
• Wide linear range: Quantification range of 5 ng/mL – 400 ng/mL, covering a broad spectrum of physiological and pathological concentrations to meet diverse sample‑testing needs.
• Excellent specificity: Rigorously validated to exhibit high specificity for target HA with negligible cross‑reactivity to related molecules, ensuring accurate results.
• One‑step protocol: Optimized workflow simplifies procedures, enhances experimental efficiency, and reduces potential handling errors.

Assay Principle

This kit employs a sandwich ELISA format. HA in the sample is captured by a pre‑coated specific antibody on the microplate, followed sequentially by a biotinylated detection antibody and streptavidin‑HRP, forming a complex. Addition of TMB substrate produces a colorimetric signal; after stopping the reaction, absorbance (OD) is measured at 450 nm. The OD value is directly proportional to the HA concentration in the sample, allowing precise quantification.

Kit Components

• Pre‑coated Microplate (Mouse HA Microplate)
• HA Standard (800 ng/mL)
• Standard Diluent
• Detection Antibody (Mouse HA Detect Antibody)
• Streptavidin‑HRP
• HRP Substrate A & B
• Stop Solution
• Wash Buffer
• Plate Covers

Specifications and Pricing

Storage and Shipping

Unopened kits should be stored protected from light at 2–8 °C and are stable for 6 months. After use, follow the instruction manual for proper storage of each component. The product is shipped on ice packs (blue ice) to maintain reagent activity.

Important Precautions

• Do not mix reagents from different lot numbers or those past their expiration date.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• To avoid cross‑contamination, use disposable pipette tips throughout the experiment.
• Unused plate strips should be promptly returned to their original packaging, sealed, and stored at 4 °C.
• Thorough mixing is critical for reliable results; recommend using a low‑speed shaker or periodic manual vortexing.
• For more robust data, perform replicate measurements (duplicate or triplicate wells) for samples and standards.

Warning: This product is for scientific research purposes only and is not intended for human or clinical diagnostic use. Users must ensure that their applications do not infringe any patents or license agreements; the manufacturer assumes no liability for any such infringement.

Note: The information above may change due to product optimization or upgrades. For the most current details, please contact EliKine.