IL-15/IL-15R Complex ELISA KIT

小鼠 IL-15/IL-15R 复合物 ELISA 试剂盒 (KTE3083-2)

产品概述

本试剂盒是一款基于双抗体夹心法原理的高灵敏度ELISA检测试剂盒，专门用于定量检测小鼠来源的细胞培养上清、血清或血浆样本中的IL-15/IL-15R复合物。作为我们EliKine™核心产品线的一员，该试剂盒经过优化，旨在为免疫学和细胞功能研究提供可靠、精准的检测工具。

主要特性

• 高灵敏度：最低检测浓度可达15.63 pg/mL。
• 宽检测范围：15.63 pg/mL - 1,000 pg/mL，适合不同浓度水平的样本检测。
• 优异特异性：经严格验证，与目标蛋白的类似物未见明显交叉反应或背景干扰。
• 即用型组分：提供预包被酶标板、冻干标准品、检测抗体等完整组分，实验流程简便。

产品背景

白细胞介素15（IL-15）是一种关键的细胞因子，在天然免疫和适应性免疫应答中扮演重要角色，尤其对NK细胞和CD8+ T细胞的发育与存活至关重要。值得注意的是，IL-15在体内通常与其特异性受体IL-15Rα形成复合物后分泌至胞外，该复合物的生物活性相较于单独的IL-15显著增强。本试剂盒直接靶向这一具有重要生物学功能的IL-15/IL-15R复合物，为深入研究其在相关疾病模型或生理过程中的作用提供了有效的检测手段。

试剂盒组分

• 小鼠 IL-15/IL-15R 复合物预包被微孔板
• 小鼠 IL-15/IL-15R 复合物标准品 (冻干)
• 标准品稀释液
• 检测缓冲液 (10×)
• 小鼠 IL-15/IL-15R 复合物检测抗体 (100×)
• 链霉亲和素-HRP (100×)
• HRP底物 (TMB)
• 终止液
• 洗涤缓冲液 (20×)
• 板盖

规格与价格

保存与运输

保存建议：未开封的试剂盒请避光保存于2-8℃，有效期24个月。启用后，请参照说明书妥善保存各组分。

运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿将不同批次的试剂或过期试剂混合使用。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 建议对样品和标准品进行复孔（双孔或三孔）检测，以提高结果准确性。
• 充分混匀是确保实验结果可靠的关键步骤。

警告：本产品仅用于科学研究，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-15/IL-15R Complex ELISA Kit (KTE3083-2)

Product Overview

This kit is a highly sensitive ELISA detection kit based on the sandwich enzyme-linked immunosorbent assay principle, specifically designed for the quantitative detection of IL-15/IL-15R complex in mouse-derived cell culture supernatants, serum, or plasma samples. As a core product of our EliKine™ line, this kit is optimized to provide a reliable and accurate detection tool for immunology and cell function research.

Key Features

• High Sensitivity: Minimum detectable concentration as low as 15.63 pg/mL.
• Broad Detection Range: 15.63 pg/mL - 1,000 pg/mL, suitable for samples with varying concentration levels.
• Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with analogs of the target protein.
• Ready-to-Use Components: Provides complete components including pre-coated microplate, lyophilized standard, detection antibody, etc., for a simplified experimental procedure.

Background

Interleukin-15 (IL-15) is a key cytokine that plays a crucial role in innate and adaptive immune responses, particularly in the development and survival of NK cells and CD8+ T cells. Notably, IL-15 is often secreted extracellularly as a complex with its specific receptor IL-15Rα, and the biological activity of this complex is significantly enhanced compared to IL-15 alone. This kit directly targets the biologically important IL-15/IL-15R complex, providing an effective detection method for in-depth study of its role in relevant disease models or physiological processes.

Kit Components

• Mouse IL-15/IL-15R Complex Pre-coated Microplate
• Mouse IL-15/IL-15R Complex Standard (Lyophilized)
• Standard Diluent
• Detection Buffer (10×)
• Mouse IL-15/IL-15R Complex Detection Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Sealers

Specifications and Price

Storage and Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8°C; shelf life is 24 months. After opening, please store components properly according to the instructions.

Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent activity.

Important Notes

• Do not mix reagents from different batches or expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• Use disposable tips during the experiment to avoid cross-contamination.
• It is recommended to perform duplicate or triplicate measurements for both samples and standards to improve result accuracy.
• Thorough mixing is a critical step to ensure reliable experimental results.

Warning: This product is for research use only, not intended for human or clinical diagnostic use.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.