小鼠高迁移率族蛋白B1 ELISA定量试剂盒(HMG1/HM

小鼠高迁移率族蛋白B1 (HMGB1) ELISA定量试剂盒 (One Step Method)

英文名称：Mouse HMGB1 ELISA Kit (One Step Method)
货号：KTE4078-1
适用物种：小鼠
检测类型：ELISA（双抗体夹心法）
样本类型：血清、血浆或其他相关生物液体

一、产品定位与核心应用

• 产品分类：核心产品，隶属细胞分析平台。
• 一级应用：ELISA，专用于蛋白类的定量分析。
• 典型研究方向：炎症响应、细胞死亡、免疫调节及相关信号通路的机制研究。

二、技术原理概述

本套件采用双抗体夹心ELISA，步骤简化为“一步法”：

1. 微孔板预包被高纯度小鼠HMGB1抗体，形成固相捕获层。
2. 加入标准品或样本，HMGB1抗原与捕获抗体形成抗原-抗体复合物。
3. 加入生物素标记的检测抗体，随后加入链霉亲和素‑HRP复合物，完成抗体‑抗原‑抗体‑酶的四元复合物。
4. 经过彻底洗涤后，加入TMB底物显色，HRP催化TMB产生蓝色，终止液将颜色转为黄色。
5. 在450 nm波长下读取OD值，依据标准曲线计算样品中HMGB1浓度。

三、主要检测指标

本试剂盒针对以下同义蛋白/别名进行定量：

• HMG1 / HMG3
• SBP1（Sulfoglucuronyl Carbohydrate Binding Protein）
• Amphoterin
• High Mobility Group Box 1 Protein

四、性能参数

检测范围	1 ng/mL – 80 ng/mL
灵敏度 (LOD)	0.2 ng/mL

五、产品优势

• 采用EliKine™技术平台，提供高灵敏度与高特异性。
• 针对HMGB1及其同源物的交叉反应已通过验证，未检出显著干扰。
• 一步法操作简化实验流程，降低人为误差。
• 标准品浓度为160 ng/mL，配套稀释缓冲液便于快速配制工作曲线。

六、试剂盒组成（每套）

• Mouse HMGB1 Microplate
• Mouse HMGB1 Standard（160 ng/mL）
• Standard Diluent
• Biotinylated HMGB1 Antibody
• HRP‑Conjugated Reagent (Streptavidin‑HRP)
• Chromogen Solution A (TMB)
• Chromogen Solution B (Stop Solution)
• Wash Buffer
• Plate Covers

七、使用注意事项

• 不同批号或已过期的试剂不可混用。
• 使用前请将试剂在室温平衡30 分钟。
• 实验过程建议使用一次性吸头，防止交叉污染。
• 未使用的板条应立即回装密封，置于4 ℃保存。
• 混匀对结果影响显著，推荐低速振荡或每10 分钟手动轻摇一次。
• 样品和标准品建议采用双孔或三孔复测，以提高数据可靠性。

八、储存与运输

• 未开封试剂盒：避光保存于2 ℃–8 ℃，保质期6个月。
• 开启后请依据说明书分别存放各组分。
• 运输方式：配备蓝色冰袋（冷链）运输，确保全程低温。

九、合规声明

本产品仅限科研用途，不适用于人类或临床诊断。使用过程中请遵守相关专利与许可证要求，制造商不承担因违规使用所产生的任何责任。

十、订购信息

规格	单价（人民币）	交货期
48 T	1998.0	期货（3 天内发货）
96 T	3198.0	期货（3 天内发货）

十一、技术支持

如需实验方案优化、数据解释或其他技术咨询，请联系我们的专业服务团队，我们将提供基于本套件的实验设计建议与操作指导。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse High Mobility Group Box 1 (HMGB1) ELISA Quantification Kit (One Step Method)

English Name: Mouse HMGB1 ELISA Kit (One Step Method)
Catalog Number: KTE4078-1
Applicable Species: Mouse
Detection Type: ELISA (Sandwich Method)
Sample Types: Serum, plasma, or other relevant biological fluids

I. Product Positioning and Core Applications

• Product Classification: Core Product, belonging to the Cell Analysis Platform.
• Primary Application: ELISA, specifically designed for quantitative analysis of proteins.
• Typical Research Areas: Inflammatory response, cell death, immune regulation, and related signaling pathway mechanism studies.

II. Technical Principle Overview

This kit employs a sandwich ELISA, with the procedure simplified to a "one-step method":

1. The microplate is pre-coated with high-purity mouse HMGB1 antibody, forming a solid-phase capture layer.
2. Add standards or samples; the HMGB1 antigen forms an antigen-antibody complex with the capture antibody.
3. Add biotin-labeled detection antibody, followed by streptavidin-HRP conjugate, completing the antibody-antigen-antibody-enzyme quaternary complex.
4. After thorough washing, add TMB substrate for color development. HRP catalyzes TMB to produce a blue color, which is converted to yellow by the stop solution.
5. Measure the OD value at 450 nm wavelength and calculate the HMGB1 concentration in the samples based on the standard curve.

III. Main Detection Targets

This kit quantifies the following synonymous proteins/aliases:

• HMG1 / HMG3
• SBP1 (Sulfoglucuronyl Carbohydrate Binding Protein)
• Amphoterin
• High Mobility Group Box 1 Protein

IV. Performance Parameters

Detection Range	1 ng/mL – 80 ng/mL
Sensitivity (LOD)	0.2 ng/mL

V. Product Advantages

• Utilizes the EliKine™ technology platform, providing high sensitivity and high specificity.
• Cross-reactivity against HMGB1 and its homologs has been validated, with no significant interference detected.
• The one-step method simplifies the experimental procedure and reduces human error.
• The standard concentration is 160 ng/mL, accompanied by dilution buffer for quick preparation of the working curve.

VI. Kit Components (Per Kit)

• Mouse HMGB1 Microplate
• Mouse HMGB1 Standard (160 ng/mL)
• Standard Diluent
• Biotinylated HMGB1 Antibody
• HRP-Conjugated Reagent (Streptavidin-HRP)
• Chromogen Solution A (TMB)
• Chromogen Solution B (Stop Solution)
• Wash Buffer
• Plate Covers

VII. Usage Precautions

• Do not mix reagents from different lots or expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable tips during the experiment to prevent cross-contamination.
• Unused strips should be immediately resealed and stored at 4°C.
• Mixing significantly affects results; low-speed shaking or manual gentle shaking every 10 minutes is recommended.
• Duplicate or triplicate wells for samples and standards are recommended to improve data reliability.

VIII. Storage and Shipping

• Unopened kit: Store protected from light at 2°C–8°C, shelf life 6 months.
• After opening, store components separately according to the instructions.
• Shipping method: Shipped with blue ice packs (cold chain) to ensure low temperature throughout.

IX. Compliance Statement

This product is intended for research use only, not for human use or clinical diagnosis. Please comply with relevant patent and licensing requirements during use. The manufacturer is not liable for any consequences arising from improper use.

X. Ordering Information

Specification	Unit Price (RMB)	Delivery Time
48 T	1998.0	Future product (ships within 3 days)
96 T	3198.0	Future product (ships within 3 days)

XI. Technical Support

For protocol optimization, data interpretation, or other technical inquiries, please contact our professional service team. We provide experimental design suggestions and operational guidance based on this kit.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.