EliKine™ 小鼠白介素1受体拮抗剂 ELISA定量试剂

EliKine™ 小鼠白介素1受体拮抗剂 ELISA定量试剂盒

英文名称：EliKine™ Mouse IL-1ra/IL-1F3 ELISA Kit | 货号：KTE3070

核心参数

• 检测范围：31.25 pg/mL-2,000 pg/mL
• 灵敏度：31.25 pg/mL
• 反应物种：小鼠
• 样本兼容性：细胞培养上清、血清、血浆
• 检测方法：双抗体夹心法
• 规格选择：48 T（¥1598） | 96 T（¥2558）

产品优势

• 采用专有链霉亲和素-HRP偶联技术，显色灵敏度提升30%
• 预包被抗体板经批次稳定性验证，板间CV值≤8%
• 标准品采用冻干稳定工艺，4℃复溶后活性保持＞24个月
• 配套10×浓缩洗涤液，支持高通量样本处理

质量控制

• 每批次试剂盒均通过标准曲线验证（R²≥0.99）
• 严格排除IL-1α、IL-1β等类似物交叉反应
• 含三重质控体系：空白对照、标准品复孔、样本复孔

操作流程优化

三步高效检测：
1. 抗原-抗体复合物形成（37℃孵育90min）
2. 生物素化抗体结合（室温振荡30min）
3. TMB显色系统定量（15min线性显色）

运输存储：蓝冰运输 | 2-8℃避光保存（开封后组分详见说明书）
应用文献：支持提供近三年20+篇小鼠炎症因子研究文献索引

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Interleukin-1 Receptor Antagonist ELISA Quantitative Kit

Catalog Number:EliKine™ Mouse IL-1ra/IL-1F3 ELISA Kit | SKU:KTE3070

Key Specifications

• Detection Range:31.25 pg/mL-2,000 pg/mL
• Sensitivity:31.25 pg/mL
• Reactivity:Mouse
• Sample Compatibility:Cell culture supernatant, serum, plasma
• Assay Principle:Sandwich ELISA
• Configuration Options:48 T (¥1598) | 96 T (¥2558)

Technical Advantages

• Proprietary streptavidin-HRP conjugation technology enhances chromogenic sensitivity by 30%
• Pre-coated plates validated for batch consistency (inter-plate CV ≤8%)
• Lyophilized stabilization maintains standard activity >24 months post-reconstitution at 4℃
• Includes 10× concentrated wash buffer for high-throughput processing

Quality Assurance

• Lot-specific standard curve validation (R²≥0.99)
• Strict exclusion of cross-reactivity with IL-1α, IL-1β analogs
• Triple QC system: Blank control, standard duplicate, sample duplicate

Streamlined Protocol

Three-step workflow:
1. Antigen-antibody complex formation (37°C incubation for 90 min)
2. Biotinylated antibody binding (room temperature with shaking for 30 min)
3. TMB chromogenic quantification (15 min linear color development)

Shipping & Storage:Blue ice shipment | 2-8°C protected from light (post-opening conditions detailed in manual)
Application References:Provides access to 20+ recent murine cytokine research citations

Note: Specifications subject to change due to product optimization. For updated information, contact Yacine Corporation.