小鼠白介素2受体 ELISA定量试剂盒(sIL-2Rα)

小鼠白介素2受体（IL‑2Rα/CD25）ELISA 定量试剂盒 (Mouse IL‑2Rα/CD25 ELISA Kit, KTE3045‑1)

产品概述

货号：KTE3045‑1 | 规格：48 条/盒（¥1 598） | 规格：96 条/盒（¥2 558）
反应性：小鼠 | 检测类型：ELISA（夹心法）
适用样本：细胞培养上清、血清、血浆

核心应用

• ELISA 应用 – 细胞分析平台的核心定量检测
• 监测可溶性 IL‑2Rα (sIL‑2Rα) 水平，评估免疫细胞激活状态
• 适用于基础研究、药物筛选及免疫学机制探索

试剂盒组成

组件	说明
Mouse sIL‑2Rα Microplate	预包被抗体孔板（96 孔）
Mouse sIL‑2Rα Standard (lyophilized)	冻干标准品，覆盖 7.81‑500 pg/mL 检测范围
Standard Diluent	标准品稀释缓冲液
Assay Buffer (10×)	工作缓冲液
Mouse sIL‑2Rα Detect Antibody (100×)	生物素化检测抗体
Streptavidin‑HRP (100×)	链霉亲和素‑HRP 结合剂
HRP Substrate (TMB)	显色底物
Stop Solution	终止液（黄酮颜色）
Wash Buffer (20×)	洗涤缓冲液
Plate Covers	防尘盖

性能指标

• 检测范围：7.81 pg/mL – 500 pg/mL
• 灵敏度（LOD）：7.81 pg/mL
• 特异性：已验证对相关蛋白无交叉反应，背景干扰极低

操作要点（建议）

• 同批次试剂请勿混用，过期试剂严禁使用。
• 使用前所有试剂在室温平衡 30 分钟。
• 实验全程建议使用一次性吸头，防止交叉污染。
• 未使用完的板需立即放回密封包装，置于 4 ℃保存。
• 混匀是关键：推荐低速振荡或每 10 分钟手动轻摇一次。
• 样品与标准建议做双孔或三孔复孔，以提高数据可靠性。

存储与运输

• 未开封试剂盒：避光保存于 2‑8 ℃，保质期 24 个月。
• 启用后各组分请参照说明书分别保存。
• 运输方式：冷链（蓝冰）保证全程 2‑8 ℃。

重要提示

仅限科研使用。本产品不用于人类或临床诊断。用户须自行确保不侵犯任何专利或许可证。本公司对因使用本产品产生的专利侵权或其他违规行为不承担责任。

技术背景

白介素 2 受体 (IL‑2R) 是免疫细胞表面的关键调节复合体，由 α (CD25)、β (CD122) 与 γ (CD132) 三条链组成。可溶性 α 链 (sIL‑2Rα) 在血清中浓度升高时，常与 T、B 细胞活化及多种自身免疫、血液系统疾病（如类风湿性关节炎、系统性红斑狼疮、特定白血病和淋巴瘤）相关。EliKine™ 试剂盒采用双抗体夹心法，通过固相捕获抗体、检测抗体+链霉亲和素‑HRP 形成免疫复合物，TMB 底物在 HRP 催化下显色，最终以 450 nm OD 值读取定量信息。

订购信息

包装规格	单价（人民币）	交货周期
48 条/盒	¥1 598.00	请咨询
96 条/盒	¥2 558.00	期货（5 天内发货）

关键字检索

IL‑2Rα、CD25、sIL‑2Rα、ELISA、细胞培养上清、血清、血浆、免疫激活、鼠模型

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Interleukin-2 Receptor Alpha (IL-2Rα/CD25) ELISA Quantitation Kit (Mouse IL-2Rα/CD25 ELISA Kit, KTE3045-1)

Product Overview

Catalog Number: KTE3045-1 | Size: 48 tests/kit (¥1,598) | Size: 96 tests/kit (¥2,558)
Reactivity: Mouse | Detection Type: ELISA (Sandwich)
Suitable Samples: Cell culture supernatant, serum, plasma

Core Applications

• ELISA Application – Core quantitative detection for cell analysis platforms
• Monitor soluble IL-2Rα (sIL-2Rα) levels to assess immune cell activation status
• Suitable for basic research, drug screening, and immunological mechanism exploration

Kit Components

Component	Description
Mouse sIL-2Rα Microplate	Pre-coated antibody plate (96 wells)
Mouse sIL-2Rα Standard (lyophilized)	Lyophilized standard, covering a detection range of 7.81-500 pg/mL
Standard Diluent	Standard diluent buffer
Assay Buffer (10×)	Working buffer
Mouse sIL-2Rα Detect Antibody (100×)	Biotinylated detection antibody
Streptavidin-HRP (100×)	Streptavidin-HRP conjugate
HRP Substrate (TMB)	Chromogenic substrate
Stop Solution	Stop solution (yellow color)
Wash Buffer (20×)	Wash buffer
Plate Covers	Dust covers

Performance Characteristics

• Detection Range: 7.81 pg/mL – 500 pg/mL
• Sensitivity (LOD): 7.81 pg/mL
• Specificity: Validated no cross-reactivity with related proteins; extremely low background interference

Operation Notes (Recommendations)

• Do not mix reagents from different lots; expired reagents are strictly prohibited from use.
• Equilibrate all reagents to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the experiment to prevent cross-contamination.
• Unused wells should be immediately returned to the sealed package and stored at 4°C.
• Thorough mixing is crucial: recommend low-speed shaking or manual gentle shaking every 10 minutes.
• Samples and standards are recommended to be run in duplicate or triplicate to improve data reliability.

Storage and Shipping

• Unopened kit: Store protected from light at 2-8°C; shelf life 24 months.
• After opening, store individual components separately according to the instructions.
• Shipping method: Cold chain (blue ice) to ensure 2-8°C throughout transit.

Important Notice

For Research Use Only. This product is not intended for human or clinical diagnostic use. The user must ensure that no patents or licenses are infringed. The company assumes no responsibility for patent infringement or other violations arising from the use of this product.

Technical Background

The interleukin-2 receptor (IL-2R) is a key regulatory complex on the surface of immune cells, composed of three chains: α (CD25), β (CD122), and γ (CD132). Elevated serum levels of the soluble α chain (sIL-2Rα) are often associated with T and B cell activation and various autoimmune and hematological diseases (such as rheumatoid arthritis, systemic lupus erythematosus, specific leukemias, and lymphomas). The EliKine™ kit employs a double-antibody sandwich method, forming an immune complex via a solid-phase capture antibody, detection antibody + streptavidin-HRP. The TMB substrate develops color catalyzed by HRP, and quantification is read as OD value at 450 nm.

Ordering Information

Package Size	Unit Price (RMB)	Delivery Lead Time
48 tests/kit	¥1,598.00	Please inquire
96 tests/kit	¥2,558.00	Future goods (shipped within 5 days)

Keyword Search

IL-2Rα, CD25, sIL-2Rα, ELISA, cell culture supernatant, serum, plasma, immune activation, mouse model

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.