B细胞活化因子酶联免疫试剂盒(小鼠) BAFF/BLyS/T

产品概览

中文名称： B细胞活化因子酶联免疫试剂盒（小鼠）
英文名称： Mouse BAFF/BLyS/TNFSF13B ELISA Kit (产品编号 KTE3096-3)
适用物种： 小鼠
核心技术： 双抗体夹心ELISA

主要应用

• 蛋白类研究，尤其是B细胞活化因子（BAFF/BLyS/TNFSF13B）的定量检测。
• ELISA平台的基础研究与药物开发筛选。
• 细胞培养上清、血清、血浆等样本的BAFF水平分析。

技术背景

BAFF（B细胞活化因子）亦称BLyS或TNFSF13B，是B细胞增殖、分化的关键刺激因子。其表达水平与免疫缺陷及多种自身免疫疾病（如系统性红斑狼疮、类风湿性关节炎）密切相关。EliKine™ 小鼠BAFF ELISA 试剂盒采用双抗体夹心法，实现对样品中BAFF的高灵敏度、特异性定量。

产品规格与价格

规格	每套含孔数	单价 (元)	货期
48孔版	48 T	1,598.0	请咨询
96孔版	96 T	2,558.0	期货（5天内发货）

检测性能

• 检测范围： 46.88 pg/mL – 3,000 pg/mL
• 检测灵敏度（LOD）： 46.88 pg/mL
• 已验证的特异性：对BAFF相似蛋白无显著交叉反应，背景噪声低。

试剂盒组分

• Mouse BAFF Microplate
• Mouse BAFF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse BAFF Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用说明要点

1. 所有试剂只能与同批号、在有效期内的对应试剂混用；不同批号之间不得混合。
2. 使用前请在室温放置30分钟，使其平衡。
3. 实验操作建议使用一次性吸头，防止交叉污染。
4. 未使用的微孔板应立即放回包装中，密封后于4℃保存。
5. 混匀对结果影响显著，推荐低速振荡或每10分钟手动轻摇一次。
6. 样品和标准建议设定双孔或三孔（复孔）以提高可靠性。

保存与运输

• 未开封试剂盒：避光保存于2‑8℃，有效期24个月。
• 已开封组分：请依据说明书中各组分的存储要求进行保存。
• 运输方式：使用蓝冰（冰袋）冷链运输，确保全程温度稳定。

注意事项与合规声明

• 本产品仅供科研使用，不得用于人类或临床诊断。
• 不建议将本试剂用于规避任何专利或许可证的行为；如因此产生的侵权或违规责任，本公司不承担任何责任。

订购与技术支持

如需了解库存、交货时间或获取技术文档，请联系我司销售代表或访问官方渠道。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Chinese Name: B Cell Activating Factor ELISA Kit (Mouse)
English Name: Mouse BAFF/BLyS/TNFSF13B ELISA Kit (Product No. KTE3096-3)
Species: Mouse
Core Technology: Sandwich ELISA

Key Applications

• Protein research, especially quantitative detection of B cell activating factor (BAFF/BLyS/TNFSF13B).
• Fundamental research and drug development screening using ELISA platforms.
• Analysis of BAFF levels in cell culture supernatants, serum, plasma, and other sample types.

Technical Background

BAFF (B cell activating factor), also known as BLyS or TNFSF13B, is a critical stimulant for B‑cell proliferation and differentiation. Its expression levels are closely linked to immunodeficiency and various autoimmune diseases (such as systemic lupus erythematosus and rheumatoid arthritis). The EliKine™ Mouse BAFF ELISA Kit employs a sandwich format to achieve high‑sensitivity and specific quantification of BAFF in samples.

Product Specifications and Pricing

Specification	Wells per Kit	Unit Price (CNY)	Delivery Lead Time
48‑well format	48 T	1,598.0	Please inquire
96‑well format	96 T	2,558.0	Future stock (shipment within 5 days)

Assay Performance

• Detection Range: 46.88 pg/mL – 3,000 pg/mL
• Limit of Detection (LOD): 46.88 pg/mL
• Verified specificity: No significant cross‑reactivity with BAFF‑related proteins and low background noise.

Kit Components

• Mouse BAFF Microplate
• Mouse BAFF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse BAFF Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Key Points of the Protocol

1. All reagents may be mixed only with corresponding reagents from the same lot and within their expiry dates; mixing across different lots is prohibited.
2. Allow reagents to equilibrate at room temperature for 30 minutes before use.
3. Use disposable pipette tips during the assay to prevent cross‑contamination.
4. Unopened microplates should be promptly returned to their packaging, sealed, and stored at 4 °C.
5. Proper mixing critically impacts results; recommend gentle rocking or manual inversion every 10 minutes.
6. Run samples and standards in duplicate or triplicate (replicate wells) to improve reliability.

Storage and Shipping

• Unopened kits: Store protected from light at 2–8 °C; shelf life 24 months.
• Opened components: Store according to the specific storage conditions outlined in the kit manual.
• Shipping method: Use cold‑chain shipping with ice packs (blue ice) to maintain temperature stability throughout transit.

Precautions and Compliance Statement

• This product is for research use only and must not be used for human or clinical diagnostic purposes.
• The reagent should not be used to circumvent any patents or licences; the company assumes no liability for any infringement or regulatory violations arising from such use.

Ordering and Technical Support

For information on stock availability, delivery timelines, or to obtain technical documentation, please contact our sales representative or visit official channels.

Note: The above information may change due to product optimization or upgrades; please consult Yakeyin for the most current details.