IL-17 ELISA KIT 白介素-17ELISA试剂盒

EliKine™ 小鼠白介素-17 (IL-17) ELISA试剂盒 (货号: KTE7012-2)

本试剂盒专为精准定量检测小鼠样本（如细胞培养上清、血清、血浆及其他生物液体）中的白介素-17 (IL-17) 蛋白水平而设计。采用成熟的夹心法ELISA原理，确保了检测的高特异性和可靠性，是免疫学、细胞因子研究及相关疾病模型分析领域的理想工具。

产品核心特点

• 高灵敏度与宽检测范围：最低检测浓度可达 4 pg/mL，定量检测范围为 7.8 pg/mL - 500 pg/mL，能够精准捕捉低丰度表达。
• 优异特异性：经严格验证，与目标蛋白的类似物无明显交叉反应或背景干扰，确保检测结果准确可信。
• 即用型完整组分：提供预包被板、标准品、检测抗体、HRP酶联物及全套缓冲液，开盒即用，简化实验流程。
• 稳定的重复性：优化的试剂配方和操作流程，为实验结果的重复性提供了有力保障。

试剂盒组分详情

每套试剂盒包含进行完整ELISA检测所需的所有核心组分：

• 小鼠源 IL-17 抗体预包被微孔板
• 小鼠源 IL-17 蛋白标准品
• 小鼠源 IL-17 检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物 (TMB)
• 反应终止液
• 洗涤缓冲液 (浓缩液)
• 封板膜

背景信息

白细胞介素-17 (IL-17A) 是一种主要由活化的T细胞（如小鼠αβ TCR+CD4-CD8-胸腺细胞及人CD4+记忆性T细胞）产生的关键促炎细胞因子。它在宿主防御、炎症反应以及多种自身免疫性疾病（如类风湿性关节炎、多发性硬化症、哮喘和狼疮）的发病机制中扮演着核心角色。该试剂盒靶向的蛋白UniProt登录号为 Q62386。

规格与订购信息

规格	货号	价格 (人民币)	货期
48 T	KTE7012-2	1598.0	现货（次日发货）
96 T	KTE7012-2	2558.0	现货（次日发货）

重要操作提示

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为获得最佳结果，强烈建议对样品和标准品进行复孔检测（双孔或三孔）。
• 实验过程中请使用一次性吸头，并确保溶液充分混匀，以避免交叉污染并保证反应均一性。
• 未使用的板条请立即放回箔袋并密封，于4℃保存。

储存与运输

• 储存：未开封的试剂盒请保存于 2-8℃。启用后，请参照说明书分别保存各组分。
• 运输：本品采用 冰袋（蓝冰） 条件运输，以确保试剂的稳定性。

警告： 本产品仅供研究使用，不适用于人类或临床诊断。

搜索别名： 白介素-17 ELISA试剂盒; 小鼠白介素-17 ELISA试剂盒; Mouse IL-17 ELISA Kit; IL17A; CTLA8; IL-17A

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Interleukin-17 (IL-17) ELISA Kit (Catalog No.: KTE7012-2)

This kit is specifically designed for the precise quantification of interleukin-17 (IL-17) protein levels in mouse samples (e.g., cell culture supernatants, serum, plasma, and other biological fluids). Utilizing a mature sandwich ELISA format, it ensures high specificity and reliability, making it an ideal tool for immunology, cytokine research, and related disease model analyses.

Core Product Features

• High Sensitivity and Wide Dynamic Range: Minimum detectable concentration as low as 4 pg/mL, quantitative range of 7.8 pg/mL – 500 pg/mL, enabling precise detection of low-abundance expression.
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity or background interference from protein homologs, guaranteeing accurate and reliable results.
• Ready‑to‑Use Complete Components: Includes pre‑coated plates, standards, detection antibodies, HRP conjugate, and a full set of buffers; ready out‑of‑the‑box to streamline workflow.
• Robust Reproducibility: Optimized reagent formulation and protocol provide strong assurance of consistent assay performance.

Kit Component Details

Each kit contains all essential components required to perform a complete ELISA assay:

• Mouse IL-17 antibody pre‑coated microplate
• Mouse IL-17 protein standard
• Mouse IL-17 detection antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate (TMB)
• Stop solution
• Wash buffer (concentrate)
• Sealing film

Background Information

Interleukin‑17A (IL‑17A) is a key pro‑inflammatory cytokine primarily produced by activated T cells (e.g., mouse αβ TCR⁺ CD4⁻ CD8⁻ thymocytes and human CD4⁺ memory T cells). It plays a central role in host defense, inflammatory responses, and the pathogenesis of various autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, asthma, and lupus. The target protein for this kit corresponds to UniProt accession Q62386.

Specifications and Ordering Information

Format	Catalog No.	Price (CNY)	Lead Time
48-well	KTE7012-2	1598.0	In stock (next‑day shipment)
96-well	KTE7012-2	2558.0	In stock (next‑day shipment)

Critical Operational Guidelines

• Do not mix reagents from different lot numbers or beyond their expiration date.
• Allow all reagents to equilibrate at room temperature for approximately 30 minutes before use.
• For optimal results, it is strongly recommended to run samples and standards in duplicate or triplicate wells.
• Use disposable pipette tips throughout the assay and ensure solutions are thoroughly mixed to avoid cross‑contamination and maintain reaction homogeneity.
• Return unused plate strips immediately to the foil pouch, seal, and store at 4 °C.

Storage and Shipping

• Storage: Store unopened kits at 2–8 °C. After opening, store individual components as instructed in the manual.
• Shipping: The product is shipped with ice packs (blue ice) to maintain reagent stability.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications.

Search aliases: Interleukin‑17 ELISA Kit; Mouse Interleukin‑17 ELISA Kit; Mouse IL‑17 ELISA Kit; IL17A; CTLA8; IL‑17A

Note: The above information may be subject to change due to product optimizations or upgrades; please contact EliKine™ for the most current details.