转化生长因子-β1酶联免疫试剂盒(小鼠) TGF-β1 EL

Mouse TGF‑β1 ELISA Kit (KTE7014‑3)

产品概述

本套装为ELISA（夹心法）专用试剂盒，针对小鼠源转化生长因子‑β1（TGF‑β1）进行定量检测。适用于细胞培养上清、血清、血浆及其他生物液体样本，广泛服务于蛋白类ELISA实验的核心需求。

核心技术特点 & 优势

• 高灵敏度：检测下限 8 pg/mL，线性检测范围 15.63 pg/mL ~ 1000 pg/mL。
• 特异性强：抗体对TGF‑β1的同源异构体和常见干扰蛋白几乎无交叉反应。
• 采用 EliKine™ 体系的预包被微孔板和Streptavidin‑HRP，提高信号一致性。
• 配套完整的缓冲液、底物及终止液，确保实验流程的完整性。

检验原理

本试剂盒基于经典夹心ELISA原理：

1. 微孔板已预包被小鼠特异性TGF‑β1捕获抗体。
2. 加入样本或标准品后，TGF‑β1被捕获。
3. 加入生物素标记的检测抗体形成二抗复合物。
4. 加入EliKine™ Streptavidin‑HRP，形成酶复合物。
5. 加入HRP底物产生可比色信号，终止反应后在450 nm读取吸光度。

试剂盒组成

• 小鼠源 TGF‑β1 抗体预包被微孔板
• 小鼠源 TGF‑β1 蛋白标准品（浓度梯度）
• 小鼠源 TGF‑β1 检测抗体（生物素标记）
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物（TMB）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防止蒸发）

适用样本类型

• 细胞培养上清
• 血清
• 血浆
• 其他生物液体（如组织匀浆、体液等）

实验操作要点

• 所有试剂应在使用前于室温（20‑25 °C）平衡30 分钟。
• 使用一次性吸头避免交叉污染。
• 未使用的微孔板应立即密封放回包装，4 °C 保存。
• 建议使用低速振荡器或每10 分钟手动轻摇，确保混匀。
• 样品及标准建议做双孔或三孔复孔以提高数据可靠性。
• 同一批次的试剂不应与其他批次或已过期试剂混用。

储存与运输

未开封试剂盒：2 °C ~ 8 °C 保存。
启用后：请参照说明书中各组分的具体保存条件。
运输方式：采用冰袋（蓝冰）冷链运输，以维持活性成分的稳定性。

安全与合规

本产品仅用于科研目的，不适用于人类或临床诊断。使用本套装进行的任何专利或许可相关活动，责任需由使用者自行承担。

技术背景

转化生长因子‑β1（TGF‑β1）是一种25 kDa的非糖基化二硫键二聚体蛋白，参与细胞增殖、分化、免疫调节以及组织修复等多重生物过程。小鼠与人类TGF‑β1的氨基酸同源性高达99%，在研究免疫抑制、伤口愈合、软骨与骨再生以及自身免疫性疾病模型中具有重要价值。
基因ID：21803 蛋白ID：P04202 UniProt 详细信息

产品规格 & 价格

规格	单价（人民币）	库存状态	货号
48 T	¥1,598.00	现货（次日发货）	KTE7014-3
96 T	¥2,558.00	现货（次日发货）	KTE7014-3

检索关键词

转化生长因子‑β1 ELISA试剂盒、Mouse TGF‑β1 ELISA Kit、TGFB1、CED、DPD1、LAP、TGFbeta、KTE7014、ELISA核心产品、细胞分析

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse TGF‑β1 ELISA Kit (KTE7014‑3)

Product Overview

This kit is an ELISA (sandwich) assay specifically designed for quantitative detection of mouse‑derived Transforming Growth Factor‑β1 (TGF‑β1). It is suitable for cell culture supernatants, serum, plasma, and other biological fluid samples, broadly serving core needs of protein‑based ELISA experiments.

Key Technical Features & Advantages

• High Sensitivity: detection limit 8 pg/mL; linear range 15.63 pg/mL ~ 1000 pg/mL.
• Strong Specificity: the antibody shows virtually no cross‑reactivity with TGF‑β1 homologous isoforms and common interfering proteins.
• Utilizes pre‑coated microplates and Streptavidin‑HRP based on the EliKine™ system, enhancing signal consistency.
• Comes with a complete set of buffers, substrate, and stop solution, ensuring workflow integrity.

Assay Principle

This kit is based on the classic sandwich ELISA principle:

1. The microplate is pre‑coated with mouse‑specific TGF‑β1 capture antibody.
2. After adding sample or standards, TGF‑β1 is captured.
3. Biotin‑labeled detection antibody is added to form a secondary antibody complex.
4. EliKine™ Streptavidin‑HRP is added to create the enzyme complex.
5. HRP substrate is added to generate a colorimetric signal; the reaction is stopped and absorbance read at 450 nm.

Kit Components

• Mouse‑derived TGF‑β1 antibody pre‑coated microplate
• Mouse‑derived TGF‑β1 protein standards (gradient concentrations)
• Mouse‑derived TGF‑β1 detection antibody (biotin‑labeled)
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate (TMB)
• Stop solution
• Wash buffer
• Plate sealing film (prevents evaporation)

Applicable Sample Types

• Cell culture supernatant
• Serum
• Plasma
• Other biological fluids (e.g., tissue homogenates, body fluids, etc.)

Critical Experimental Steps

• All reagents should be equilibrated at room temperature (20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unused microplates should be immediately sealed, returned to their packaging, and stored at 4 °C.
• It is recommended to use a low‑speed shaker or gently tap manually every 10 minutes to ensure mixing.
• Samples and standards are recommended to be run in duplicate or triplicate wells to enhance data reliability.
• Reagents from the same lot should not be mixed with other lots or expired reagents.

Storage and Shipping

Unopened kit: store at 2 °C ~ 8 °C.
After opening: refer to the instruction manual for specific storage conditions of each component.
Shipping method: shipped on cold chain using ice packs (blue ice) to maintain stability of active ingredients.

Safety and Compliance

This product is intended for research purposes only and is not suitable for human or clinical diagnostics. Any patent or licensing activities conducted with this kit are the sole responsibility of the user.

Technical Background

Transforming Growth Factor‑β1 (TGF‑β1) is a 25 kDa non‑glycosylated disulfide‑linked dimeric protein involved in numerous biological processes including cell proliferation, differentiation, immune regulation, and tissue repair. The amino‑acid sequence of mouse and human TGF‑β1 shares up to 99% identity, making it highly valuable in studies of immunosuppression, wound healing, cartilage and bone regeneration, and autoimmune disease models.
Gene ID: 21803 Protein ID: P04202 UniProt details

Product Specifications & Pricing

Specification	Unit Price (CNY)	Stock Status	Item Number
48 T	¥1,598.00	In stock (next‑day shipping)	KTE7014-3
96 T	¥2,558.00	In stock (next‑day shipping)	KTE7014-3

Search Keywords

Transforming Growth Factor‑β1 ELISA kit, Mouse TGF‑β1 ELISA Kit, TGFB1, CED, DPD1, LAP, TGFbeta, KTE7014, ELISA core product, cell analysis

Note: The above information may change due to product optimization, upgrades, or other factors; please contact Yakeyin Co. for the latest details.