IL-1β ELISA KIT 白介素-1βELISA试剂盒

产品概述

产品名称：IL-1β ELISA KIT（白介素‑1β ELISA 试剂盒，大鼠）
英文名称：Rat IL‑1β ELISA Kit
货号：KTE9001‑2
适用物种：大鼠

核心应用

• 蛋白类检测
• ELISA 夹心法定量分析
• 细胞培养上清、血清、血浆及其他生物液体中的 IL‑1β 测定

产品分类与定位

本试剂盒属于 核心产品，隶属 细胞分析 中心的 ELISA 类别，专为研究实验室提供高灵敏度的炎症因子定量方案。

检测试剂盒组成

• 大鼠源 IL‑1β 抗体预包被微孔板
• 大鼠源 IL‑1β 蛋白标准品
• 大鼠源 IL‑1β 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

技术特点 & 优势

• 采用 EliKine™ 夹心技术，实现 32 pg/mL 的检测灵敏度。
• 检测范围宽广：62.5 pg/mL – 4000 pg/mL。
• 高特异性，未发现明显的交叉反应或背景干扰。
• 兼容常规酶标仪（450 nm）读取。
• 标准品与检测抗体均来源于同一物种（大鼠），降低基质效应。

检测范围 & 灵敏度

检测范围：62.5 pg/mL – 4000 pg/mL
最低检测限制（LOD）：32 pg/mL

操作建议（合理扩展）

• 样品及标准品建议采用双孔或三孔复孔设置，以提升结果的可重复性。
• 每 10 分钟轻轻摇匀（低速振荡或手动），确保反应均匀。
• 使用一次性吸头避免交叉污染；未用完的板条请密封回包装，在 4 ℃保存。
• 实验结束后，建议在 30 分钟内读取吸光度，以避免底物自行衰减。

注意事项

• 不同批号或超期试剂请勿混用。
• 使用前请在室温平衡 30 分钟。
• 全部操作应在洁净环境中进行，防止样品被污染。

储存与运输

储存：未开封试剂盒请置于 2‑8 ℃；启用后请参照说明书分别保存各组分。
运输：采用蓝冰（冰袋）冷链运输，确保全程低温。

警告声明

本产品仅限于科研使用，不适用于人类或临床诊断。使用本产品进行的任何专利或许可证相关行为，均由使用方自行承担风险，供应商不承担责任。

背景信息

白细胞介素‑1（IL‑1）是一类 17 kDa 的胞外多肽，分为 IL‑1α 与 IL‑1β 两种。IL‑1β 由活化的巨噬细胞分泌，其前体经 caspase‑1 水解后生成活化形式，是炎症反应的关键介导者，参与细胞增殖、分化及凋亡等多种生物过程。

基因与蛋白信息

• 基因 ID：24494
• 蛋白质 ID：Q5BKB0
• 别名：IL1B; IL‑1; IL1‑BETA; IL1F2; catabolin; preinterleukin 1 beta; pro‑interleukin‑1‑beta
• Uniprot 链接：Q5BKB0

规格与价格

规格	含量	单价 (¥)	货期
48 T	48 试剂盒	1,598.0	现货（次日发货）
96 T	96 试剂盒	2,558.0	现货（次日发货）

技术支持

我们的专业技术团队可提供实验设计、数据解析及使用规范的指导，欢迎随时联系。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: IL-1β ELISA KIT (Interleukin-1β ELISA Kit, Rat)
English Name: Rat IL-1β ELISA Kit
Catalog Number: KTE9001-2
Applicable Species: Rat

Core Applications

• Protein Detection
• Quantitative Analysis by ELISA Sandwich Assay
• Measurement of IL-1β in Cell Culture Supernatant, Serum, Plasma, and Other Biological Fluids

Product Classification & Positioning

This kit is categorized as a Core Product within the ELISA category of the Cell Analysis center, specifically designed to provide high-sensitivity quantitative solutions for inflammatory factors in research laboratories.

Kit Components

• Pre-coated Microplate with Rat IL-1β Antibody
• Rat IL-1β Protein Standard
• Rat IL-1β Detection Antibody
• EliKine™ Streptavidin-HRP
• Standard Diluent
• Assay Buffer
• HRP Substrate
• Stop Solution
• Wash Buffer
• Plate Sealer

Technical Features & Advantages

• Utilizes EliKine™ Sandwich Technology, achieving a sensitivity of 32 pg/mL.
• Broad detection range: 62.5 pg/mL – 4000 pg/mL.
• High specificity with no significant cross-reactivity or background interference observed.
• Compatible with standard microplate readers (450 nm).
• Both standard and detection antibody are derived from the same species (Rat), reducing matrix effects.

Detection Range & Sensitivity

Detection Range: 62.5 pg/mL – 4000 pg/mL
Limit of Detection (LOD): 32 pg/mL

Operation Recommendations (Reasonable Extension)

• It is recommended to set up samples and standards in duplicate or triplicate wells to enhance result reproducibility.
• Gently agitate (low-speed shaking or manually) every 10 minutes to ensure uniform reaction.
• Use disposable tips to avoid cross-contamination; unused strips should be resealed and stored at 4°C.
• After the assay, read the absorbance within 30 minutes to avoid substrate self-degradation.

Precautions

• Do not mix reagents from different lot numbers or expired kits.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• All operations should be performed in a clean environment to prevent sample contamination.

Storage & Shipping

Storage: Unopened kits should be stored at 2-8°C; after opening, store components separately as per the instructions.
Shipping: Shipped with blue ice (ice packs) via cold chain to ensure low temperature throughout transit.

Warning Statement

This product is for research use only. Not intended for human or clinical diagnostic use. Any activities related to patents or licenses conducted using this product are undertaken at the user's own risk; the supplier assumes no liability.

Background Information

Interleukin-1 (IL-1) is a class of 17 kDa extracellular polypeptides, comprising IL-1α and IL-1β. IL-1β is secreted by activated macrophages. Its precursor is cleaved by caspase-1 to generate the active form. It is a key mediator of inflammatory responses and involved in various biological processes such as cell proliferation, differentiation, and apoptosis.

Gene & Protein Information

• Gene ID: 24494
• Protein ID: Q5BKB0
• Aliases: IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
• Uniprot Link: Q5BKB0

Specifications & Pricing

Specification	Content	Unit Price (¥)	Lead Time
48 T	48 Tests	1,598.0	In Stock (Ships Next Day)
96 T	96 Tests	2,558.0	In Stock (Ships Next Day)

Technical Support

Our professional technical team can provide guidance on experimental design, data analysis, and usage protocols. Feel free to contact us at any time.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YourCompany.