IL-12 p70 ELISA KIT 白介素-12 p70

Human IL-12 p70 ELISA Kit (人白介素-12 p70 ELISA试剂盒)

货号: KTE6020-2

产品概述

本试剂盒是一种基于夹心法原理的酶联免疫吸附测定（ELISA）试剂盒，专门用于定量检测人源样本中的白细胞介素-12 p70 (IL-12 p70) 蛋白水平。该试剂盒经过优化，适用于分析多种生物样本，包括细胞培养上清、血清、血浆以及其他生物液体，为免疫学和细胞功能研究提供可靠的数据支持。

产品特点与优势

• 高灵敏度与特异性：采用EliKine™技术，检测灵敏度可达4 pg/mL，对目标蛋白具有高度特异性，在测试中未发现与类似物有明显的交叉反应或背景干扰。
• 宽检测范围：提供7.8 pg/mL 至 500 pg/mL的宽线性检测范围，确保对不同浓度水平的样本都能获得准确的定量结果。
• 即用型组分：试剂盒提供预包被微孔板、即用型标准品和检测抗体等完整组分，简化实验流程，节省准备时间。
• 批次稳定性：严格的质量控制确保不同批次间试剂盒性能的一致性和可靠性。

背景信息

白细胞介素-12 (IL-12) 是一种主要由抗原呈递细胞（如树突状细胞、巨噬细胞）产生的异源二聚体细胞因子，由IL-12A (p35) 和 IL-12B (p40) 两个亚基组成。其生物活性形式IL-12 p70在先天性和适应性免疫应答中扮演着核心角色，特别是通过促进初始T细胞向Th1细胞分化，刺激T细胞和自然杀伤（NK）细胞的活性和功能。此外，IL-12 p70与自身免疫性疾病的发生发展密切相关，使其成为免疫学研究中的重要靶点。

UniProt 蛋白数据库链接： P29459 (IL12A_HUMAN)

试剂盒组分

• 人源 IL-12 p70 抗体预包被微孔板
• 人源 IL-12 p70 蛋白标准品
• 人源 IL-12 p70 检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物
• 反应终止液
• 洗涤缓冲液（浓缩）
• 封板膜

详细组分信息请参阅产品说明书。

技术规格

检测方法	夹心法 ELISA
反应性	人 (Human)
样本类型	细胞培养上清、血清、血浆、其他生物液体
检测范围	7.8 pg/mL - 500 pg/mL
灵敏度	4 pg/mL

订购信息

规格	价格 (元)	货期
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

保存与运输

• 保存建议：请将未开封的试剂盒储存于2-8℃。启用后，请参照说明书分别保存各组分。
• 运输条件：全程冰袋（蓝冰）运输，以确保试剂性能稳定。

重要注意事项

• 请勿将不同批次的试剂或已过有效期的试剂混合使用。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对标准品和样品进行复孔检测（如双孔或三孔）。

警告： 本品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，我方不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IL-12 p70 ELISA Kit (Human Interleukin-12 p70 ELISA Kit)

Catalog No.: KTE6020-2

Product Overview

This kit is a sandwich‑based enzyme‑linked immunosorbent assay (ELISA) designed for the quantitative detection of human interleukin‑12 p70 (IL‑12 p70) protein levels in biological samples. The kit has been optimized for a variety of matrices, including cell culture supernatants, serum, plasma, and other biological fluids, providing reliable data for immunology and cellular function studies.

Key Features and Benefits

• High Sensitivity and Specificity: Utilizes EliKine™ technology with a detection sensitivity as low as 4 pg/mL, exhibiting high specificity for the target protein and no significant cross‑reactivity or background interference with related molecules.
• Wide Dynamic Range: Provides a linear quantification range of 7.8 pg/mL to 500 pg/mL, ensuring accurate quantitation across varying sample concentrations.
• Ready‑to‑Use Components: The kit includes pre‑coated microplates, ready‑to‑use standards, and detection antibodies, streamlining workflow and reducing preparation time.
• Batch‑to‑Batch Consistency: Rigorous quality control ensures consistent performance and reliability across different production lots.

Background Information

Interleukin‑12 (IL‑12) is a heterodimeric cytokine primarily produced by antigen‑presenting cells such as dendritic cells and macrophages, composed of the IL‑12A (p35) and IL‑12B (p40) subunits. The biologically active heterodimer, IL‑12 p70, plays a central role in innate and adaptive immune responses, notably by driving naïve T‑cell differentiation toward the Th1 phenotype and enhancing the activity and function of T cells and natural killer (NK) cells. Moreover, IL‑12 p70 is closely implicated in the pathogenesis of autoimmune diseases, making it a critical target in immunological research.

UniProt protein database link: P29459 (IL12A_HUMAN)

Kit Components

• Human IL‑12 p70 antibody pre‑coated microplate
• Human IL‑12 p70 protein standard
• Human IL‑12 p70 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer (concentrate)
• Plate sealing film

For detailed component information, refer to the product manual.

Technical Specifications

Assay Method	Sandwich ELISA
Species Reactivity	Human
Sample Types	Cell culture supernatants, serum, plasma, other biological fluids
Detection Range	7.8 pg/mL - 500 pg/mL
Sensitivity	4 pg/mL

Ordering Information

Format	Price (CNY)	Lead Time
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Storage and Shipping

• Storage recommendation: Store unopened kits at 2–8 °C. After opening, follow the manual for storage of individual components.
• Shipping condition: Ship on ice packs (blue ice) throughout transit to maintain reagent stability.

Critical Precautions

• Do not mix reagents from different batches or expired reagents.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Return unused strip wells to the original pouch, seal, and store at 4 °C.
• Thorough mixing is critical for reliable results; use a low‑speed shaker or manually invert periodically.
• For more reliable data, perform replicate measurements of standards and samples (e.g., duplicate or triplicate wells).

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or license agreements. We assume no liability for any patent infringement or other violations arising from use of this product.

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakeyin Company for the most current details.