EliKine™ 人载脂蛋白A1 ELISA定量试剂盒

EliKine™ 人载脂蛋白A1 ELISA定量试剂盒

产品核心参数

货号：KTE3149 | 规格：48T（1598元）、96T（2558元）

检测范围：0.63-40 ng/mL | 灵敏度：0.63 ng/mL

◼️ 产品优势

• 双抗体夹心法确保检测特异性，消除交叉反应
• 多样本兼容：血清/血浆/细胞培养上清
• 预包被微孔板缩短实验准备时间
• 含冻干标准品与即用型HRP底物

◼️ 实验方案

三步高效流程：

1. 目标蛋白捕获（2小时）
2. 生物素化抗体结合（1小时）
3. 链霉亲和素-HRP信号放大（<30分钟）

◼️ 质控保障

• 批次间CV值<10%
• 标准曲线R²≥0.99
• 含20×浓缩洗液避免边缘效应

◼️ 应用场景

心血管研究：

• HDL代谢机制研究
• 动脉粥样硬化风险评估
• 抗凝血功能关联分析

注意事项：

• 启用后组分需按说明书分装保存
• 推荐复孔检测（CV<15%）
• 运输全程2-8℃冷链

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Human Apolipoprotein A1 ELISA Quantitation Kit

Core Product Parameters

Catalog No.: KTE3149 | Specification: 48T (¥1598), 96T (¥2558)

Detection Range: 0.63-40 ng/mL | Sensitivity: 0.63 ng/mL

◼️ Product Advantages

• Sandwich ELISA ensures detection specificity and eliminates cross-reactivity
• Multiple sample compatibility: Serum/plasma/cell culture supernatant
• Pre-coated microplate reduces experimental preparation time
• Includes lyophilized standards and ready-to-use HRP substrate

◼️ Experimental Protocol

Three-step efficient workflow:

1. Target protein capture (2 hours)
2. Biotinylated antibody binding (1 hour)
3. Streptavidin-HRP signal amplification (<30 minutes)

◼️ Quality Control

• Inter-assay CV <10%
• Standard curve R²≥0.99
• Includes 20× concentrated wash buffer to prevent edge effects

◼️ Applications

Cardiovascular research:

• HDL metabolism mechanism studies
• Atherosclerosis risk assessment
• Anticoagulant function correlation analysis

Notes:

• Components must be aliquoted and stored according to the manual after opening
• Recommended duplicate detection (CV<15%)
• Maintain 2-8℃ cold chain during transportation

Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult ACROBiosystems.