IGFBP-6 ELISA KIT 胰岛素样生长因子结合蛋白

产品概述

英文名称：Human IGFBP-6 ELISA Kit
中文名称：IGFBP-6 ELISA KIT 胰岛素样生长因子结合蛋白6 ELISA试剂盒（人）
货号：KTE3106-2
产品分类：核心产品 → 细胞分析 → ELISA
检测类型：酶联免疫吸附测定（ELISA，夹心法）
适用样本：细胞培养上清、血清、血浆

规格与包装

包装规格	含量 (孔数)	单价（人民币）	交货周期
48 T	48孔	1,598.00	期货（5天内发货）
96 T	96孔	2,558.00	期货（5天内发货）

试剂盒组成

• Human IGFBP-6 Microplate
• Human IGFBP-6 Standard (冻干粉)
• Assay Buffer (10×)
• Human IGFBP-6 Detect Antibody (100×, 生物素化)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

检测原理

本套件采用双抗体夹心法进行人IGFBP-6定量：

1. 预包被抗IGFBP-6抗体的酶标板捕获样本中IGFBP-6。
2. 加入生物素化检测抗体形成“捕获‑抗原‑检测”复合物。
3. 链霉亲和素‑HRP 与生物素特异结合，引入酶活性。
4. 加入TMB底物，在HRP催化下产生蓝色，终止液后变为黄色。
5. 在450 nm波长读取OD值，浓度通过与标准曲线比较获得。

关键优势

• 高灵敏度：检测下限 62.5 pg/mL。
• 宽检测范围：62.5 pg/mL – 4,000 pg/mL，满足低至中等丰度样本的需求。
• 特异性强：未观察到显著交叉反应或背景干扰。
• 采用EliKine™专利技术，实验过程可重复性好。

检测范围与灵敏度

检测范围：62.5 pg/mL – 4,000 pg/mL
灵敏度（LOD）：62.5 pg/mL

使用说明（简要）

1. 将所有试剂在使用前在室温平衡30 分钟。
2. 按照说明书配置标准品系列，建议每点设置双孔或三孔复孔。
3. 加入样本或标准品至预包被孔中，37 °C孵育1 h（或说明书推荐时间）。
4. 依次加入检测抗体、Streptavidin‑HRP、TMB底物和终止液，每步均严格洗涤。
5. 使用酶标仪在450 nm读取OD值，使用配套软件绘制标准曲线并计算样本浓度。

保存与运输

• 未开封的试剂盒请避光保存于2‑8 °C，保质期24 个月。
• 开启后各组分请依据说明书中的储存条件分别保存。
• 运输采用蓝冰冷链，确保全程低温。

注意事项

• 不同批号或过期的试剂不可混用。
• 实验操作建议使用一次性吸头，防止交叉污染。
• 未使用的酶标板请立即放回封装，4 °C保存。
• 充分混匀对结果影响显著，推荐低速振荡或每10 分钟手动摇匀。
• 标准品和样本建议做复孔，以提升数据可靠性。

背景信息

胰岛素样生长因子（IGF）结合蛋白家族包括六种高亲和力IGFBP及多种低亲和力相关蛋白。IGFBP-6在人体内主要以高亲和力结合IGF‑II，是IGF‑II的特异性抑制剂，且在不依赖IGF的情况下参与细胞增殖、存活、分化及迁移等生物过程。该蛋白的表达水平常用于研究前列腺、骨骼、神经等组织的生理与病理状态。

法律声明

本产品仅供科研使用，不适用于临床诊断或人类治疗。使用本套件进行的任何专利相关活动均由用户自行负责，供应商不承担因专利侵权或其他违规行为导致的责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Human IGFBP-6 ELISA Kit
Chinese Name: IGFBP-6 ELISA KIT Insulin‑like Growth Factor Binding Protein 6 ELISA Kit (Human)
Catalog No.: KTE3106-2
Product Category: Core Products → Cell Analysis → ELISA
Assay Type: Enzyme‑Linked Immunosorbent Assay (ELISA, sandwich format)
Applicable Samples: Cell culture supernatants, serum, plasma

Specifications and Packaging

Package Specification	Content (Number of wells)	Unit Price (CNY)	Delivery Lead Time
48 T	48 wells	1,598.00	Spot (shipped within 5 days)
96 T	96 wells	2,558.00	Spot (shipped within 5 days)

Kit Components

• Human IGFBP-6 Microplate
• Human IGFBP-6 Standard (lyophilized powder)
• Assay Buffer (10×)
• Human IGFBP-6 Detection Antibody (100×, biotinylated)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Assay Principle

This kit quantifies human IGFBP-6 using a dual‑antibody sandwich format:

1. Pre‑coated anti‑IGFBP-6 antibody on the microplate captures IGFBP-6 present in the samples.
2. Add biotinylated detection antibody to form a “capture‑antigen‑detection” complex.
3. Streptavidin‑HRP binds specifically to biotin, introducing enzymatic activity.
4. Add TMB substrate; HRP catalyzes a color change to blue, which turns yellow upon addition of stop solution.
5. Read absorbance at 450 nm; concentration is derived by comparison to the standard curve.

Key Advantages

• High sensitivity: limit of detection 62.5 pg/mL.
• Wide detection range: 62.5 pg/mL – 4,000 pg/mL, suitable for low to moderate abundance samples.
• Strong specificity: no significant cross‑reactivity or background interference observed.
• Utilizes EliKine™ patented technology, offering excellent assay reproducibility.

Detection Range and Sensitivity

Detection Range: 62.5 pg/mL – 4,000 pg/mL
Sensitivity (LOD): 62.5 pg/mL

Brief Protocol

1. Equilibrate all reagents at room temperature for 30 minutes before use.
2. Prepare the standard series as per the manual; it is recommended to run each point in duplicate or triplicate.
3. Add samples or standards to the pre‑coated wells and incubate at 37 °C for 1 hour (or as recommended).
4. Sequentially add detection antibody, Streptavidin‑HRP, TMB substrate, and stop solution, performing thorough washes between each step.
5. Measure absorbance at 450 nm using a microplate reader; generate the standard curve and calculate sample concentrations using the accompanying software.

Storage and Transport

• Store unopened kits protected from light at 2‑8 °C; shelf life is 24 months.
• After opening, store each component according to the conditions specified in the instruction manual.
• Transport uses blue‑ice cold chain to maintain low temperature throughout.

Precautions

• Do not mix reagents from different lots or expired batches.
• Use disposable pipette tips to avoid cross‑contamination.
• Return unused microplates to their packaging promptly and store at 4 °C.
• Thorough mixing markedly impacts results; recommend gentle vortexing or manual inversion every 10 minutes.
• Run standards and samples in replicate wells to improve data reliability.

Background Information

The insulin‑like growth factor (IGF) binding protein family comprises six high‑affinity IGFBPs and several low‑affinity related proteins. IGFBP‑6 in humans primarily binds IGF‑II with high affinity, acting as a specific inhibitor of IGF‑II, and also participates in cell proliferation, survival, differentiation, and migration independent of IGF. Expression levels of this protein are commonly used to study physiological and pathological states of prostate, bone, nerve, and other tissues.

Legal Disclaimer

This product is for research use only and is not intended for clinical diagnosis or human therapy. Any patent‑related activities conducted with this kit are the sole responsibility of the user; the supplier assumes no liability for patent infringement or other violations.

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakein Co., Ltd.