alpha-Galactosidase A/GLA ELIS

Human alpha-Galactosidase A (GLA) ELISA Kit

货号: KTE3141-2 | 核心产品

产品概述

本试剂盒为EliKine™系列双抗体夹心法ELISA试剂盒，专门用于定量检测人血清、血浆及细胞培养上清等样本中的α-半乳糖苷酶A（GLA）含量。该试剂盒具有高灵敏度和高特异性，检测范围为0.5 ng/mL至32 ng/mL，灵敏度可达0.5 ng/mL，适用于法布里病（Fabry Disease）等相关研究的精准定量分析。

主要特点与优势

• 采用夹心法ELISA原理，特异性强，对目标蛋白类似物无明显交叉反应
• 优化的试剂组分，包括预包被微孔板、冻干标准品、HRP标记检测抗体等，确保实验稳定可靠
• 适用于多种样本类型，包括细胞培养上清、血清和血浆
• 提供详细的实验操作指南与保存建议，便于实验室标准化操作

试剂盒组分

• Human GLA Pre-coated Microplate
• Human GLA Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• HRP-conjugated Human GLA Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

技术参数

检测范围	0.5 ng/mL - 32 ng/mL
灵敏度	0.5 ng/mL
样本类型	细胞培养上清、血清、血浆
反应种属	人
保存条件	未开封试剂盒避光保存于2-8℃，有效期24个月
运输条件	冰袋运输（蓝冰）

背景信息

α-半乳糖苷酶A（GLA）是糖基水解酶27家族成员，其活性缺乏与法布里病（FD）密切相关。FD是一种X连锁遗传性鞘脂代谢疾病，特征为糖脂在多种组织中沉积，导致多器官病变。GLA的定量检测对于FD的病理研究、酶替代疗法评估及临床前研究具有重要意义。

规格与价格

• 规格1: 48 T - 价格: 1598.0元
• 规格2: 96 T - 价格: 2558.0元（期货，5天内发货）

注意事项

• 请勿混合使用不同批号或过期试剂
• 使用前需室温平衡30分钟，避免交叉污染
• 建议进行复孔检测以提高结果可靠性
• 未使用的板条需密封保存于4℃

本产品仅供研究使用，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human alpha‑Galactosidase A (GLA) ELISA Kit

Item No.: KTE3141-2 | Core Product

Product Overview

This kit is part of the EliKine™ dual‑antibody sandwich ELISA series, specifically designed for the quantitative determination of α‑galactosidase A (GLA) in human serum, plasma, and cell culture supernatants. The kit offers high sensitivity and specificity, with a detection range of 0.5 ng/mL to 32 ng/mL and a limit of detection of 0.5 ng/mL. It is suitable for precise quantitative analyses in Fabry disease (FD) and related research.

Key Features and Advantages

• Sandwich ELISA format provides strong specificity with minimal cross‑reactivity to homologous proteins
• Optimized reagent components—including pre‑coated microplates, lyophilized standards, and HRP‑conjugated detection antibodies—ensure stable and reliable performance
• Compatible with multiple sample types, such as cell culture supernatant, serum, and plasma
• Comes with detailed operating instructions and storage recommendations for standardized laboratory workflow

Kit Components

• Human GLA Pre‑coated Microplate
• Human GLA Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• HRP‑conjugated Human GLA Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Technical Specifications

Detection Range	0.5 ng/mL – 32 ng/mL
Sensitivity	0.5 ng/mL
Sample Types	Cell culture supernatant, serum, plasma
Species Reactivity	Human
Storage Conditions	Unopened kits should be protected from light and stored at 2–8 °C; shelf life 24 months
Shipping Conditions	Ice‑pack (blue ice) shipments

Background

α‑Galactosidase A (GLA) belongs to the glycoside hydrolase family 27. Deficiency of GLA activity is closely associated with Fabry disease (FD), an X‑linked lysosomal storage disorder characterized by glycosphingolipid accumulation in multiple tissues, leading to multi‑organ pathology. Quantitative measurement of GLA is essential for FD pathophysiology studies, evaluation of enzyme replacement therapy, and preclinical research.

Specifications and Pricing

• Specification 1: 48 tests – Price: ¥1,598.0
• Specification 2: 96 tests – Price: ¥2,558.0 (future order, shipment within 5 days)

Precautions

• Do not combine reagents from different lot numbers or expired reagents
• Allow reagents to equilibrate to room temperature for 30 minutes before use; avoid cross‑contamination
• Duplicate wells are recommended to increase result reliability
• Unused plates should be sealed and stored at 4 °C

This product is intended for research use only and is not suitable for human or clinical diagnostic applications.

Note: The information above may be subject to change due to product optimization or upgrades; please contact Yakoin for the most current details.