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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 检测范围:
1.25 U/mL-100 U/mL
- 检测方法:
双抗体夹心法
- 应用:
ELISA检测
- 适应物种:
人
- 标记物:
Cancer Antigen 125; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125
- 样本:
血清、血浆、组织、细胞上清及相关液体样本
- 灵敏度:
0.25 U/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥1998.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥3198.0 |
Human Carbohydrate Antigen 125 (CA125) ELISA Kit (One Step Method)
人糖抗原125 (CA125) ELISA试剂盒(一步法)
货号:KTE4054-2
产品概述
本试剂盒是一款基于双抗体夹心法原理的高灵敏度ELISA检测试剂盒,专门用于定量检测人血清、血浆、组织匀浆及细胞上清液等样本中的糖抗原125(CA125)浓度。CA125作为一种重要的肿瘤标志物,在卵巢癌等相关肿瘤的筛查、辅助诊断及疗效监测中具有重要临床参考价值。
核心特性与优势
- 高灵敏度与特异性:最低检测限达0.25 U/mL,检测范围为1.25 - 100 U/mL,采用EliKine™技术平台,有效避免与类似物的交叉反应,背景干扰低。
- 一步法操作:优化后的实验流程简化了操作步骤,缩短了孵育时间,提升了检测效率,适合中小通量样本检测。
- 优异的重复性:试剂盒组分经过严格质控,批间差小,支持复孔检测,确保实验结果的稳定可靠。
- 广泛的样本兼容性:适用于多种生物样本类型,包括血清、血浆、组织匀浆及细胞培养上清等。
技术参数
| 检测方法 | 双抗体夹心法 ELISA |
| 反应种属 | 人(Human) |
| 样本类型 | 血清、血浆、组织匀浆、细胞上清及相关液体样本 |
| 检测范围 | 1.25 U/mL - 100 U/mL |
| 灵敏度 | 0.25 U/mL |
| 孵育时间 | 约2.5小时(一步法优化) |
试剂盒组分
- 人CA125预包被微孔板
- 人CA125标准品(200 U/mL)
- 标准品稀释液
- 人CA125检测抗体
- 链霉亲和素-HRP
- HRP底物A、B液
- 终止液
- 洗涤缓冲液(浓缩)
- 板盖
保存与运输
保存条件:未开封试剂盒避光保存于2-8℃,有效期6个月;开封后请按说明书要求保存各组分。
运输方式:冰袋(蓝冰)运输,确保试剂活性。
规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T | 1998.0 | 期货(3个工作日内发货) |
| 96 T | 3198.0 | 期货(3个工作日内发货) |
注意事项
- 请勿混合使用不同批号或过期试剂。
- 使用前请将所有组分室温平衡30分钟,并充分混匀。
- 建议使用复孔(双孔或三孔)检测以提高数据可靠性。
- 本产品仅供科研使用,不适用于临床诊断。
应用领域:肿瘤标志物研究、卵巢癌相关机制探索、生物标志物验证等生命科学研究。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Human Carbohydrate Antigen 125 (CA125) ELISA Kit (One Step Method)
Human Carbohydrate Antigen 125 (CA125) ELISA Kit (One Step Method)
Catalog Number:KTE4054-2
Product Overview
This kit is a highly sensitive ELISA detection kit based on the double-antibody sandwich principle, specifically designed for the quantitative determination of Carbohydrate Antigen 125 (CA125) concentration in samples such as human serum, plasma, tissue homogenates, and cell culture supernatants. As an important tumor marker, CA125 holds significant clinical reference value in the screening, auxiliary diagnosis, and therapeutic monitoring of ovarian cancer and other related tumors.
Core Features & Advantages
- High Sensitivity & Specificity: Minimum detection limit of 0.25 U/mL, detection range of 1.25 - 100 U/mL. Utilizes the EliKine™ technology platform to effectively avoid cross-reactivity with analogs and minimize background interference.
- One-Step Procedure: The optimized protocol simplifies operational steps, shortens incubation time, and improves detection efficiency, making it suitable for small to medium sample throughput.
- Excellent Reproducibility: Kit components undergo strict quality control with low inter-assay variation. Supports duplicate or triplicate testing to ensure stable and reliable results.
- Broad Sample Compatibility: Applicable to various biological sample types, including serum, plasma, tissue homogenates, and cell culture supernatants.
Technical Parameters
| Assay Method | Double-Antibody Sandwich ELISA |
| Reactivity | Human |
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Supernatants, and related liquid samples |
| Detection Range | 1.25 U/mL - 100 U/mL |
| Sensitivity | 0.25 U/mL |
| Incubation Time | Approximately 2.5 hours (One-Step optimized) |
Kit Components
- Pre-coated Microplate (Human CA125)
- Human CA125 Standard (200 U/mL)
- Standard Diluent
- Detection Antibody (Human CA125)
- Streptavidin-HRP
- HRP Substrate Solution A & B
- Stop Solution
- Wash Buffer (Concentrated)
- Plate Sealer
Storage & Shipping
Storage: Store the unopened kit protected from light at 2-8°C; valid for 6 months. After opening, store components as instructed in the manual.
Shipping: Shipped with ice packs (blue ice) to ensure reagent activity.
Specifications & Price
| Specification | Price (RMB) | Lead Time |
|---|---|---|
| 48 T | 1998.0 | Future Goods (Ships within 3 business days) |
| 96 T | 3198.0 | Future Goods (Ships within 3 business days) |
Precautions
- Do not mix reagents from different lots or use expired reagents.
- Allow all components to equilibrate to room temperature for 30 minutes before use and mix thoroughly.
- Duplicate or triplicate testing is recommended to enhance data reliability.
- This product is for research use only. Not intended for diagnostic use.
Applications: Tumor marker research, exploration of ovarian cancer-related mechanisms, biomarker validation, and other life science research.
Note: The above information is subject to change due to product optimization and upgrades. Please consult Ya Keyin Company for specific details.
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文献和实验葡萄糖来源 AGEs ELISA 检测试剂盒 ( Glucose-derived AGEs ELISA Kit ) 研 究 背 景 越来越多的证据指出晚期糖基化终端产物( AGEs )修饰蛋白严重影响到老龄化人群的健康问题。 AGEs 是葡萄糖与自由的蛋白质氨基基团发生非酶促糖基化反应的终端不可逆代谢产物的总称。其在人体内的积累会引发组织或器官一系列的病理性变化,从而导致白内障、阿尔茨海默氏病、骨关节炎、心功
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
【求助】如何提取细胞中的cAMP?和普通蛋白的裂解buffer一样么?
出售,不是基于ELISA法的,比ELISA法更方便。 苏叶 我用的是125I的RIA kit,我们核同位素室的老师说,照常处理你的细胞,离心去上清后加入0.4ml 1M的高氯酸就可以了。不过要预实验一下,摸索浓度。 我这几天也在做细胞内cAMP和cGMP检测。 sosorunning 普通的Lysis buffer 就可以,但是你得加IBMX抑制PDE的活性。不知道你是想做western还是干吗? 如果你是要测
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