TNF-β细胞因子,重组人肿瘤坏死因子β,重组蛋白,E.co

重组人TNF-β蛋白 (Animal-Free), His标签

货号：PRP1123
产品名称：Human TNF-β Protein, His tag (Animal-Free)

本品为采用无动物源（Animal-Free）表达系统生产的重组人肿瘤坏死因子β（TNF-β）蛋白，具有高纯度、高生物活性的特点，专为严格的细胞培养及相关生物研究需求设计。

产品特性

• 高比活性：经功能活性检测，本品能够在使用DactinomycinD的L929细胞中诱导细胞毒性，其ED₅₀值< 3 pg/mL，比活性高达> 3.3 × 10⁸ IU/mg，确保了其在细胞实验中的高效性。
• 超高纯度：经SDS-PAGE验证，蛋白纯度> 98%，有效减少杂蛋白对实验结果的影响。
• 极低内毒素：通过LAL法测定，每微克蛋白中内毒素含量< 0.1 EU，非常适合对内毒素敏感的细胞学研究。
• 明确的分子结构：蛋白序列为人源TNF-β (Leu 35-Leu 205)，由171个氨基酸组成，预测分子量为18.65 kDa，与理论值一致。

产品描述

重组人TNF-β（亦称淋巴毒素-α，LTA）是一种由205个氨基酸组成的细胞因子，分子量约为22 kDa。它与TNF-α同属于肿瘤坏死因子超家族。TNF-β通过结合淋巴毒素-β受体（LtβR），激活经典和非经典的NF-κB信号通路，在细胞凋亡、炎症信号传导以及淋巴器官发育等生理和病理过程中扮演关键角色。本品在大肠杆菌中重组表达，并在C端带有His标签，便于后续的检测或纯化操作。

产品形式与保存

• 产品形式：白色或微白色冻干粉末。
• 冻干缓冲液：无菌的20mM柠檬酸钠，0.2 M NaCl, pH 3.5。
• 复溶建议：推荐使用本公司提供的专用复溶缓冲液进行复溶，建议复溶浓度不低于100 µg/mL，客户可根据具体实验需求进行进一步稀释。
• 保存条件：冻干粉在-20°C条件下可稳定保存1年。复溶后于2-8°C可保存1周。若在含有载体蛋白（如0.1% BSA）的缓冲液中进一步稀释并分装，于-20°C或-80°C下可保存3-6个月。请避免反复冻融。

使用注意事项

• 开盖前，请先短暂离心试管，以确保粉末聚集于管底。
• 本品仅供研究使用，不适用于人类或临床诊断。

运输与规格

运输条件：蓝冰运输。

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	3469	请咨询
1 mg	请咨询	请咨询

基因ID：4049 | UniProt ID：P01374

警告：用户应自行确保产品的使用不侵犯任何专利权或许可证。对于因使用本产品而可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human TNF-β Protein (Animal-Free), His‑tag

Product No.：PRP1123
Product Name：Human TNF-β Protein, His tag (Animal-Free)

This product is a recombinant human tumor necrosis factor β (TNF‑β) protein produced using an animal‑free expression system, characterized by high purity and high bioactivity, and is specifically designed for stringent cell‑culture and related biomedical research applications.

Product Features

• High specific activity: Functional assays demonstrate that this product induces cytotoxicity in L929 cells in the presence of Actinomycin D, with an ED₅₀ of < 3 pg/mL and a specific activity exceeding > 3.3 × 10⁸ IU/mg, ensuring high efficacy in cellular experiments.
• Ultra‑high purity: SDS‑PAGE analysis confirms protein purity > 98%, effectively minimizing the impact of contaminating proteins on experimental results.
• Extremely low endotoxin: Determined by LAL assay, endotoxin levels are < 0.1 EU per µg protein, making it ideal for endotoxin‑sensitive cellular studies.
• Defined molecular structure: The protein sequence corresponds to human TNF‑β (Leu 35–Leu 205), comprising 171 amino acids with a predicted molecular weight of 18.65 kDa, matching the theoretical value.

Product Description

Recombinant human TNF‑β (also known as lymphotoxin‑α, LTA) is a cytokine consisting of 205 amino acids with an approximate molecular weight of ~22 kDa. It belongs to the tumor necrosis factor superfamily alongside TNF‑α. TNF‑β binds to the lymphotoxin‑β receptor (LTβR) and activates both canonical and non‑canonical NF‑κB signaling pathways, playing pivotal roles in apoptosis, inflammatory signaling, and lymphoid organ development in physiological and pathological contexts. This product is expressed recombinantly in Escherichia coli and carries a C‑terminal His‑tag to facilitate downstream detection or purification.

Product Form and Storage

• Product form: White to off‑white lyophilized powder.
• Lyophilization buffer: Sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
• Reconstitution recommendation: We recommend reconstituting with the proprietary buffer provided by the company; reconstitution concentration should be ≥100 µg/mL, and users may further dilute according to specific experimental needs.
• Storage conditions: Lyophilized powder is stable for 1 year at –20 °C. After reconstitution, the solution can be stored at 2–8 °C for up to 1 week. If further diluted and aliquoted in a buffer containing a carrier protein (e.g., 0.1 % BSA), aliquots are stable for 3–6 months at –20 °C or –80 °C. Avoid repeated freeze‑thaw cycles.

Usage Precautions

• Before opening, briefly centrifuge the vial to ensure the powder settles at the bottom.
• This product is for research use only and not intended for human or clinical diagnostic applications.

Shipping and Specifications

Shipping condition: Dry ice shipment.

Specification	Price (CNY)	Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1498	In stock (next‑day shipment)
100 µg	3469	Please inquire
1 mg	Please inquire	Please inquire

Gene ID: 4049 | UniProt ID: P01374

Warning: Users are responsible for ensuring that their use of this product does not infringe any patents or licenses. The company assumes no liability for any patent infringement or other violations that may arise from product use.

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakoin Co. for the most current details.