FGF-1细胞因子,重组人酸性成纤维细胞生长因子,重组蛋白,

Human FGF1/aFGF Protein, His tag (Animal-Free)

货号：PRP1008

产品类型：重组人酸性成纤维细胞生长因子（Animal-Free表达系统）

产品概述

本品为高纯度、高活性重组人源FGF-1蛋白，采用无动物源成分（Animal-Free）的大肠杆菌表达系统生产，C端携带His标签，适用于细胞培养及相关体外研究应用。该蛋白通过严格的质控检测，包括SDS-PAGE纯度分析、内毒素检测及功能活性验证，确保其在细胞增殖与分化研究中的可靠性和一致性。

关键特性

• 来源：人源FGF1 (UniProt: P05230-1)，序列区域：Phe 16 - Asp 155
• 分子量：16.77 kDa（预测），SDS-PAGE验证
• 纯度：＞98% （SDS-PAGE检测）
• 活性：ED₅₀ < 0.3 ng/mL，促进3T3细胞增殖，比活性 > 1×10⁶ IU/mg
• 内毒素：＜0.1 EU/μg（LAL法测定）
• 表达系统：E.coli，无动物成分
• 产品形式：冻干粉（冻干缓冲液：PBS，pH 8.0）

应用方向

• 细胞增殖与分化研究
• 血管生成（Angiogenesis）相关实验
• 组织修复与再生机制研究
• 干细胞培养与类器官模型构建

使用建议

• 复溶：推荐使用配套复溶缓冲液（Reconstitution Buffer），浓度不低于 100 µg/mL
• 保存：冻干粉-20°C可保存1年；复溶后2-8°C可存放1周。如需长期保存，建议在含稳定剂（如0.1% BSA、10% FBS、5% HSA或5%海藻糖）的缓冲液中分装，并存放于-20°C或-80°C，避免反复冻融
• 操作提示：使用前请离心，开盖需谨慎

背景信息

FGF-1（Fibroblast Growth Factor-1，别名HBGF-1或ECGF-beta）是一种由巨噬细胞分泌的生长因子，具有促进内皮细胞增殖与血管新生的功能。其在组织损伤修复和细胞周期调控中发挥关键作用。FGF-1通过与细胞膜上的FGFR（Fibroblast Growth Factor Receptor）结合，诱导内吞并激活下游信号通路。

基因ID：2246 | UniProt ID：P05230

规格与价格

运输与警告

运输条件：冰袋运输（蓝冰）

警告：本品仅供研究使用，不适用于人类或临床诊断。用户应自行负责确保在使用过程中不违反相关专利或许可协议。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human FGF1/aFGF Protein, His tag (Animal-Free)

Catalog Number: PRP1008

Product Type: Recombinant Human Acidic Fibroblast Growth Factor (Animal-Free Expression System)

Product Overview

This product is a high-purity, high-activity recombinant human FGF-1 protein produced using an Animal-Free E. coli expression system, with a His tag at the C-terminus. It is suitable for cell culture and related in vitro research applications. The protein undergoes strict quality control testing, including SDS-PAGE purity analysis, endotoxin testing, and functional activity verification, ensuring reliability and consistency in cell proliferation and differentiation studies.

Key Features

• Source: Human FGF1 (UniProt: P05230-1), Sequence Region: Phe 16 - Asp 155
• Molecular Weight: 16.77 kDa (Predicted), Verified by SDS-PAGE
• Purity: >98% (by SDS-PAGE)
• Activity: ED₅₀ < 0.3 ng/mL, promotes 3T3 cell proliferation, Specific Activity > 1×10⁶ IU/mg
• Endotoxin: <0.1 EU/μg (determined by LAL method)
• Expression System: E. coli, Animal-Free
• Form: Lyophilized powder (Lyophilized from PBS, pH 8.0)

Applications

• Cell Proliferation and Differentiation Studies
• Angiogenesis-Related Experiments
• Tissue Repair and Regeneration Mechanism Research
• Stem Cell Culture and Organoid Model Construction

Usage Suggestions

• Reconstitution: Recommended to use the provided Reconstitution Buffer to a concentration not less than 100 µg/mL
• Storage: Lyophilized powder can be stored at -20°C for 1 year; after reconstitution, it can be stored at 2-8°C for 1 week. For long-term storage, it is recommended to aliquot in a buffer containing stabilizers (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose) and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles.
• Handling Note: Centrifuge before use, open the vial carefully.

Background Information

FGF-1 (Fibroblast Growth Factor-1, also known as HBGF-1 or ECGF-beta) is a growth factor secreted by macrophages, which promotes endothelial cell proliferation and angiogenesis. It plays a key role in tissue damage repair and cell cycle regulation. FGF-1 binds to the Fibroblast Growth Factor Receptor (FGFR) on the cell membrane, inducing endocytosis and activating downstream signaling pathways.

Gene ID: 2246 | UniProt ID: P05230

Specifications & Pricing

Shipping & Caution

Shipping Condition: Shipped with ice packs (blue ice)

Caution: This product is for research use only. Not intended for human or clinical diagnostic use. The user is responsible for ensuring that their use does not violate any relevant patents or licensing agreements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.