IL-25 - Human IL-25 Protein, H

重组人IL-25蛋白（无动物源，His标签） - PRP1941

产品概述

本品为高纯度重组人源白细胞介素25（IL-25）蛋白，采用无动物源表达系统生产，C端携带His标签，适用于多种细胞培养及免疫学研究应用。该蛋白经严格质控，具备卓越的生物活性与批次稳定性，是研究炎症反应、免疫调节及细胞信号通路的理想工具。

产品特性

• 货号: PRP1941
• 蛋白名称: Human IL-25 Protein, His tag (Animal-Free)
• 种属来源: 人（Human）
• 表达系统: 大肠杆菌（E. coli，无动物源）
• 氨基酸序列: Tyr33-Gly177（UniProt ID: Q9H293）
• 分子量: 约19 kDa
• 纯度: >98%（SDS-PAGE检测）
• 内毒素: <0.1 EU/µg（LAL法测定）
• 标签: C-terminal His tag
• 产品形式: 冻干粉（冻干缓冲液为无菌PBS, pH 8.0）

生物活性验证

本品生物活性通过以下方法严格验证：

• 诱导人外周血单个核细胞（PBMCs）分泌IL-8，ED50 < 5 ng/mL
• 诱导HT29细胞分泌CXCL1，ED50 < 1 ng/mL

应用领域

• 细胞因子功能研究与信号通路分析
• 炎症与免疫调节机制研究
• 中性粒细胞趋化作用实验
• NF-κB通路激活实验
• ELISA、Western Blot、细胞刺激等体外实验

使用建议

• 复溶: 推荐使用配套复溶缓冲液，浓度不低于100 µg/mL，后续可根据实验需求稀释。
• 保存条件: 冻干粉-20°C保存1年；复溶后2-8°C可保存1周；稀释后分装-20°C或-80°C保存3-6个月，避免反复冻融。
• 注意事项: 使用前请离心，避免剧烈震荡。

规格与价格

规格	价格（元）	货期
5 µg	588	现货，次日发货
20 µg	1498	请咨询
100 µg	3589	请咨询
1 mg	请咨询	请咨询

背景信息

白细胞介素25（IL-25，又名IL17E）是一种多效细胞因子，兼具促炎与抗炎功能。它通过激活NF-κB信号通路，刺激IL-8等趋化因子产生，在中性粒细胞募集和2型免疫应答中起关键作用。

运输与警告

• 运输条件: 蓝冰冰袋运输
• 警告: 本品仅供科研使用，不适用于人体或临床诊断。用户应确保使用符合相关法律法规及知识产权要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human IL-25 Protein (Animal-Free, His Tag) - PRP1941

Product Overview

This product is a high‑purity recombinant human interleukin‑25 (IL‑25) protein produced using an animal‑free expression system, with a C‑terminal His tag. It is suitable for a variety of cell‑culture and immunology research applications. The protein undergoes stringent quality control, exhibiting excellent bioactivity and batch‑to‑batch consistency, making it an ideal tool for studying inflammatory responses, immune regulation, and cellular signaling pathways.

Product Specifications

• Item Number: PRP1941
• Protein Name: Human IL-25 Protein, His tag (Animal-Free)
• Species Origin: Human
• Expression System: Escherichia coli (Animal-Free)
• Amino Acid Sequence: Tyr33‑Gly177 (UniProt ID: Q9H293)
• Molecular Weight: ~19 kDa
• Purity: >98% (SDS‑PAGE analysis)
• Endotoxin: <0.1 EU/µg (LAL assay)
• Tag: C‑terminal His tag
• Product Form: Lyophilized powder (lyophilization buffer: sterile PBS, pH 8.0)

Bioactivity Validation

The bioactivity of this product has been rigorously validated by the following assays:

• Induction of IL‑8 secretion from human peripheral blood mononuclear cells (PBMCs), ED50 < 5 ng/mL
• Induction of CXCL1 secretion from HT‑29 cells, ED50 < 1 ng/mL

Applications

• Cytokine functional studies and signaling pathway analysis
• Inflammation and immune‑regulation mechanism studies
• Neutrophil chemotaxis assays
• NF‑κB pathway activation assays
• In‑vitro assays such as ELISA, Western blot, cell stimulation, etc.

Recommended Usage

• Reconstitution: Recommend using the provided reconstitution buffer at a concentration of ≥100 µg/mL; subsequent dilutions can be made according to experimental needs.
• Storage Conditions: Lyophilized powder – store at –20 °C for up to 1 year; after reconstitution – store at 2‑8 °C for up to 1 week; diluted aliquots – store at –20 °C or –80 °C for 3‑6 months, avoid repeated freeze‑thaw cycles.
• Precautions: Centrifuge before use and avoid vigorous shaking.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, next‑day shipping
20 µg	1498	Please inquire
100 µg	3589	Please inquire
1 mg	Please inquire	Please inquire

Background

Interleukin‑25 (IL‑25, also known as IL‑17E) is a pleiotropic cytokine with both pro‑inflammatory and anti‑inflammatory properties. By activating the NF‑κB signaling pathway, it stimulates the production of chemokines such as IL‑8, playing a pivotal role in neutrophil recruitment and type‑2 immune responses.

Shipping and Warning

• Shipping Conditions: Transport in blue ice packs
• Warning: This product is for research use only and is not suitable for human administration or clinical diagnostics. Users must ensure compliance with applicable laws, regulations, and intellectual‑property requirements.

Note: The above information may change due to product optimization or upgrades; please contact Yakein Company for the most current details.