Lymphopoietin 1(LP-1), pre-B-c

重组人白介素‑7（Human IL‑7 Protein, His tag, Animal‑Free）

产品概述

本品为源自人IL‑7（氨基酸序列 Asp26‑His177，UniProt ID P13232）的重组细胞因子，C 端融合 6×His 标记，采用 大肠杆菌（E.coli）无动物源表达体系生产，适用于细胞培养、免疫调节及肿瘤研究等多种细胞学实验。

核心参数

• 蛋白长度（分子量）：18.3 kDa（SDS‑PAGE 还原条件下约 19 kDa）
• 活性：在 PHA 激活的人外周血单个核细胞（PBMC）增殖实验中，ED₅₀ < 0.8 ng/mL；比活力 > 1 × 10⁸ IU/mg
• 纯度：≥ 95 %（SDS‑PAGE 鉴定）
• 内毒素：≤ 0.1 EU/µg（LAL 法测定）
• 表达宿主：大肠杆菌

关键特性 & 优势

• 动物源自由：全程无动物成分，降低批间变异风险。
• 高活性：ED₅₀ 低于 0.8 ng/mL，适用于低浓度刺激实验。
• 低内毒素水平：确保在敏感细胞系（如淋巴细胞）使用时不致产生非特异性炎症反应。
• 即用型冻干粉：经 0.2 µm 过滤，储存和运输稳定。
• 多用途：支持 B/T 细胞增殖、前体 B 细胞分化、免疫细胞功能评估及肿瘤细胞系的IL‑7通路研究。

技术规格

使用说明

1. 使用前先在 4 °C 离心 5 min（≈ 10,000 g），去除可能的沉淀。
2. 建议使用本公司提供的 Reconstitution Buffer（1× PBS, pH 8.0）复溶，复溶浓度不低于 100 µg/mL。
3. 复溶后可根据实验需求在含有载体蛋白或稳定剂的缓冲体系中进一步稀释（如 0.1 % BSA、10 % FBS、5 % HSA），以提高蛋白的稳定性。
4. 为避免吸附和降解，建议在使用前轻柔混匀，避免剧烈漩涡或反复冻融。

保存与运输

• 冻干状态：-20 °C 保存 ≤ 1 年。
• 复溶后短期保存：2 °C‑8 °C 可保存约 1 周。
• 长期保存（稀释液）：在含 0.1 % BSA、10 % FBS 或 5 % HSA 的缓冲液中，分装后置于 -20 °C 或 -80 °C，可保存 3‑6 个月，尽量避免反复冻融。
• 运输方式：全程冰袋（蓝冰）冷链运输，确保产品在送达时保持冻干状态。

适用研究方向

IL‑7 在以下领域常被作为实验工具使用：

• T 细胞和 B 细胞的体外增殖与分化
• 淋巴细胞亚群功能分析（如记忆 T 细胞、调节性 T 细胞）
• 造血干/前体细胞培养与维持
• 免疫检查点或细胞因子网络的机制研究
• IL‑7 信号通路在恶性肿瘤（胶质母细胞瘤、黑色素瘤、白血病等）中的作用探索

注意事项 & 警告

• 本产品为 研究级（仅限实验室使用），不适用于临床诊断或治疗。
• 请遵守相关专利和许可规定，使用本产品不构成对任何专利的侵权行为的授权。
• 在任何使用场景中，因违规或专利争议产生的法律责任，由使用方自行承担。
• 打开包装后请及时离心并在推荐浓度范围内使用，以保证实验效果。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin‑7 (Human IL‑7 Protein, His tag, Animal‑Free)

Product Overview

This product is a recombinant cytokine derived from human IL‑7 (amino acid sequence Asp26‑His177, UniProt ID P13232), with a C‑terminal fused 6×His tag. It is produced using an E. coli animal‑free expression system and is suitable for various cytological experiments, including cell culture, immunomodulation, and oncology research.

Core Parameters

• Protein Length (Molecular Weight): 18.3 kDa (approximately 19 kDa under reducing SDS‑PAGE conditions)
• Activity: ED₅₀ < 0.8 ng/mL in a PHA‑stimulated human peripheral blood mononuclear cell (PBMC) proliferation assay; specific activity > 1 × 10⁸ IU/mg
• Purity: ≥ 95% (as determined by SDS‑PAGE)
• Endotoxin: ≤ 0.1 EU/µg (determined by LAL assay)
• Expression Host: E. coli

Key Features & Advantages

• Animal‑Free: Entirely free of animal components, reducing the risk of batch-to-batch variation.
• High Activity: ED₅₀ below 0.8 ng/mL, suitable for low-concentration stimulation experiments.
• Low Endotoxin Level: Ensures no non-specific inflammatory responses when used with sensitive cell lines (e.g., lymphocytes).
• Ready-to-Use Lyophilized Powder: Filtered through 0.2 µm, stable for storage and transportation.
• Versatile: Supports B/T cell proliferation, pre-B cell differentiation, immune cell functional assessment, and IL‑7 pathway research in tumor cell lines.

Technical Specifications

Usage Instructions

1. Before use, centrifuge at 4 °C for 5 min (≈ 10,000 g) to remove any potential precipitate.
2. Reconstitution is recommended using the provided Reconstitution Buffer (1× PBS, pH 8.0) to a final concentration not less than 100 µg/mL.
3. After reconstitution, further dilution can be performed in buffer systems containing carrier proteins or stabilizers (e.g., 0.1% BSA, 10% FBS, 5% HSA) to enhance protein stability.
4. To avoid adsorption and degradation, mix gently before use and avoid vigorous vortexing or repeated freeze-thaw cycles.

Storage & Shipping

• Lyophilized State: Store at -20 °C for ≤ 1 year.
• Short-Term Storage After Reconstitution: Store at 2 °C‑8 °C for approximately 1 week.
• Long-Term Storage (Diluted): Aliquot and store at -20 °C or -80 °C in buffer containing 0.1% BSA, 10% FBS, or 5% HSA for 3‑6 months; avoid repeated freeze-thaw cycles.
• Shipping Method: Shipped with ice packs (blue ice) via cold chain to ensure the product remains lyophilized upon arrival.

Applicable Research Areas

IL‑7 is commonly used as an experimental tool in the following fields:

• In vitro proliferation and differentiation of T cells and B cells
• Functional analysis of lymphocyte subsets (e.g., memory T cells, regulatory T cells)
• Culture and maintenance of hematopoietic stem/progenitor cells
• Mechanistic studies of immune checkpoints or cytokine networks
• Exploration of the role of the IL‑7 signaling pathway in malignancies (e.g., glioblastoma, melanoma, leukemia)

Precautions & Warnings

• This product is for research grade (laboratory use only) and is not intended for clinical diagnosis or therapy.
• Please comply with relevant patent and licensing regulations. Use of this product does not constitute authorization for any patent infringement.
• The user assumes all legal liability arising from non-compliance or patent disputes in any usage scenario.
• After opening, promptly centrifuge and use within the recommended concentration range to ensure experimental efficacy.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.