bone morphogenetic protein 4,

重组小鼠骨形态发生蛋白4 (BMP-4) His标签蛋白

产品编号: PRP1227
英文名称: Mouse BMP-4 Protein, His tag

产品概述

本品为大肠杆菌表达的重组小鼠骨形态发生蛋白4（Bone Morphogenetic Protein 4, BMP-4），带有His标签，氨基酸序列来源于小鼠BMP-4（UniProt ID: P21275）的Lys303-Arg408片段。该重组蛋白由106个氨基酸组成，预测分子量为12.1 kDa，经SDS-PAGE验证纯度超过98%，适用于多种细胞培养及分子生物学研究应用。

产品特性

• 高纯度: 经SDS-PAGE检测，纯度 > 98%
• 低内毒素: 每微克蛋白质内毒素含量 < 0.1 EU（LAL法测定）
• 蛋白长度: 106个氨基酸
• 预测分子量: 12.1 kDa
• 表达系统: 大肠杆菌
• 标签类型: His标签
• 产品形式: 冻干粉（冻干缓冲液为无菌PBS，pH 4.0）

应用方向

BMP-4属于TGF-β超家族成员，是一种多功能配体蛋白，在血管平滑肌细胞增殖、内皮细胞迁移与血管生成等过程中发挥重要作用。它也被认为是血管钙化（尤其在动脉粥样硬化中）的标志物和驱动因子，广泛用于骨分化、血管生物学及炎症相关机制研究。

使用注意事项

• 使用前请先离心，确保产品完全收集。
• 推荐使用原装复溶缓冲液（Reconstitution Buffer）进行复溶，浓度不低于100 µg/mL。
• 复溶后可根据实验需要进行进一步稀释。
• 避免反复冻融，建议分装保存。

保存与运输条件

• 保存条件: 冻干粉建议在-20℃以下干燥环境中保存；复溶后可在4℃保存2–7天，长期储存请置于-20℃以下，建议添加0.1% HSA或BSA作为稳定剂。
• 运输条件: 蓝冰冰袋运输。

规格与价格

注意: 本品仅供科研使用，不适用于人体或临床诊断。使用者应负责确保其应用不违反相关专利或许可证规定。

基因ID: 12159
别名: BMP4, BMP-4, BMP 4, BMP-2B, DVR4, bone morphogenetic protein 4
UniProt 链接: P21275

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Bone Morphogenetic Protein 4 (BMP-4) His-tagged Protein

Catalog Number: PRP1227
English Name: Mouse BMP-4 Protein, His tag

Product Overview

This product is a recombinant mouse Bone Morphogenetic Protein 4 (BMP-4) expressed in E. coli, featuring a His tag. The amino acid sequence corresponds to the Lys303-Arg408 fragment of mouse BMP-4 (UniProt ID: P21275). This recombinant protein consists of 106 amino acids with a predicted molecular weight of 12.1 kDa. It has a purity of >98% as determined by SDS-PAGE and is suitable for various cell culture and molecular biology research applications.

Product Features

• High Purity: >98% purity as determined by SDS-PAGE
• Low Endotoxin: < 0.1 EU per µg of protein (determined by LAL method)
• Protein Length: 106 amino acids
• Predicted Molecular Weight: 12.1 kDa
• Expression System: E. coli
• Tag Type: His tag
• Product Form: Lyophilized powder (lyophilized from sterile PBS, pH 4.0)

Applications

BMP-4, a member of the TGF-β superfamily, is a multifunctional ligand protein that plays important roles in processes such as vascular smooth muscle cell proliferation, endothelial cell migration, and angiogenesis. It is also recognized as a marker and driver of vascular calcification (particularly in atherosclerosis) and is widely used in research on bone differentiation, vascular biology, and inflammation-related mechanisms.

Usage Instructions

• Centrifuge the vial prior to opening to ensure all product is collected.
• It is recommended to reconstitute the lyophilized protein using the provided Reconstitution Buffer to a concentration not less than 100 µg/mL.
• After reconstitution, the product can be further diluted as required for specific experiments.
• Avoid repeated freeze-thaw cycles. Aliquotting is recommended for storage.

Storage and Shipping Conditions

• Storage Conditions: The lyophilized powder should be stored in a dry environment below -20°C. After reconstitution, it can be stored at 4°C for 2–7 days. For long-term storage, keep below -20°C, and it is advised to add 0.1% HSA or BSA as a stabilizer.
• Shipping Conditions: Shipped on blue ice packs.

Specifications and Pricing

Note: This product is intended for research use only and is not suitable for human or clinical diagnostic use. The user is responsible for ensuring that its application does not infringe upon relevant patents or license agreements.

Gene ID: 12159
Aliases: BMP4, BMP-4, BMP 4, BMP-2B, DVR4, bone morphogenetic protein 4
UniProt Link: P21275

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.