β-Nerve Growth Factor, NGF-β,

Human β-NGF Protein, His Tag (Animal-Free)

货号：PRP1061

本品为重组人源β-神经生长因子（β-NGF），采用无动物源表达系统生产，带有His标签，具备高纯度与高生物活性，适用于细胞培养及相关神经科学研究。

产品概述

β-Nerve Growth Factor（β-NGF）是神经营养因子家族的重要成员，在神经细胞生长、分化与存活中发挥关键作用。本品通过大肠杆菌表达系统重组制备，序列为人源β-NGF（Ser122-Ala241），C端融合6×His标签，分子量为13.5 kDa，纯度经SDS-PAGE验证大于98%。

产品特性

• 来源：人源（Homo sapiens）
• 表达宿主：大肠杆菌（Escherichia coli）
• 标签：C端6×His
• 蛋白长度：120个氨基酸
• 预测分子量：13.5 kDa
• 纯度：＞98%（SDS-PAGE检测）
• 内毒素水平：＜0.1 EU/μg（LAL法检测）

生物活性

通过TF-1细胞增殖实验测定，其ED50＜0.7 ng/mL，特异性活性＞1×10⁶ IU/mg。

产品形式与复溶建议

本品为冻干粉末，由20 mM柠檬酸钠和0.2 M NaCl（pH 3.5）缓冲液经0.2 μm过滤后冻干制成。建议复溶时使用无菌ddH₂O，浓度不低于100 μg/mL，并在室温静置至少20分钟以确保充分溶解。请勿振荡，以免影响蛋白活性。

保存与运输条件

• 保存条件：冻干粉-20°C可保存1年；复溶后于2-8°C可保存1周；稀释后（如含0.1% BSA）于-20°C或-80°C可保存3-6个月。避免反复冻融。
• 运输条件：冰袋（蓝冰）运输。

应用领域

β-NGF在神经科学研究中具有广泛应用，包括但不限于：

• 神经元细胞培养与分化研究
• 神经退行性疾病模型建立（如阿尔茨海默病）
• 周围神经病变相关机制研究
• 免疫调节研究（如T细胞IFN-γ表达调控）

注意事项

本品仅供科研使用，不适用于人体或临床诊断。使用前请务必离心，复溶时避免剧烈振荡。

规格与价格

规格	价格（元）	货期
20 μg	588	现货，次日发货
100 μg	1498	请咨询
500 μg	3998	请咨询
1 mg	请咨询	请咨询

背景信息

β-NGF（UniProt ID: P01138）是一种神经营养因子，可形成二聚体并参与调控交感神经元和感觉神经元的生长与分化。近年来，重组β-NGF在阿尔茨海默病、糖尿病神经病变等疾病的临床前研究中显示出潜在应用价值。

更多别名：β-Nerve Growth Factor, NGF-β, 重组人神经生长因子-β

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human β-NGF Protein, His Tag (Animal-Free)

Product No.: PRP1061

This product is recombinant human β‑nerve growth factor (β‑NGF), produced using an animal‑free expression system, carries a His‑tag, and exhibits high purity and high biological activity, suitable for cell culture and related neuroscience research.

Product Overview

β‑Nerve Growth Factor (β‑NGF) is a key member of the neurotrophin family and plays critical roles in neuronal growth, differentiation, and survival. This product is recombinantly expressed in Escherichia coli, comprising the human β‑NGF sequence (Ser122‑Ala241) with a C‑terminal 6×His tag. Molecular weight is 13.5 kDa, and purity is >98% as verified by SDS‑PAGE.

Product Features

• Source: Human (Homo sapiens)
• Expression Host: Escherichia coli
• Tag: C‑terminal 6×His
• Protein Length: 120 amino acids
• Theoretical Molecular Weight: 13.5 kDa
• Purity: >98% (SDS‑PAGE)
• Endotoxin Level: <0.1 EU/µg (LAL assay)

Bioactivity

Determined by TF‑1 cell proliferation assay, with an ED₅₀ < 0.7 ng/mL and specific activity > 1×10⁶ IU/mg.

Formulation and Reconstitution Guidance

The product is supplied as lyophilized powder, prepared from a buffer of 20 mM sodium citrate and 0.2 M NaCl (pH 3.5) that has been 0.2 µm filtered prior to lyophilization. Reconstitute with sterile ddH₂O to a concentration of at least 100 µg/mL, and allow the solution to stand at room temperature for ≥ 20 minutes to ensure complete dissolution. Do not vortex to avoid compromising protein activity.

Storage and Shipping Conditions

• Storage: Lyophilized powder – 20 °C, up to 1 year; reconstituted solution – 2–8 °C, up to 1 week; diluted (e.g., containing 0.1 % BSA) – ‑20 °C or ‑80 °C, 3–6 months. Avoid repeated freeze‑thaw cycles.
• Shipping: Transport on ice packs (blue ice).

Application Areas

β‑NGF has broad applications in neuroscience research, including but not limited to:

• Neuronal cell culture and differentiation studies
• Establishment of neurodegenerative disease models (e.g., Alzheimer’s disease)
• Investigation of peripheral neuropathy mechanisms
• Immunomodulation research (e.g., regulation of T‑cell IFN‑γ expression)

Precautions

This product is for research use only and is not intended for human or clinical diagnostic applications. Centrifuge before use and avoid vigorous shaking during reconstitution.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
20 µg	588	In stock, next‑day shipment
100 µg	1498	Please inquire
500 µg	3998	Please inquire
1 mg	Please inquire	Please inquire

Background Information

β‑NGF (UniProt ID: P01138) is a neurotrophin that can form dimers and regulates the growth and differentiation of sympathetic and sensory neurons. Recently, recombinant β‑NGF has shown potential in preclinical studies of Alzheimer’s disease, diabetic neuropathy, and other disorders.

Additional Synonyms: β‑Nerve Growth Factor, NGF‑β, recombinant human nerve growth factor‑β

Note: The above information may change as the product is optimized or upgraded; please contact Yakoin for the most current details.