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- β-NGF
- 2026年01月31日
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| Source | Human Cells |
| Description | Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed. |
| Names | Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB |
| Accession # | P01138 |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0. |
| Shipping | The product is shipped at ambient temperature. |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Amino Acid Sequence |
SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVD SGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVR
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| Background | Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. |
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文献和实验Identification of Neurotrophic Factors by Molecular Biological Techniques
Many proteins have “neurotrophic” activities in in vitro or in vivo test systems. Originally these were discovered as certain neurotrophic activities with unknown molecular structures. Nerve growth factor (NGF), brain derived neurotrophic
1. Nerve growth factor (2.5S NGF, Bioproducts for Science). Prepare 1 mg/ml stock in 50 mM NaAc, pH 5.0. Sterile filter and store as 5 ul aliquots in 15 ml conical tubes at -80o C. 2. NGF medium: Add 1 ml of F12K medium to each tube of NGF aliquot
Production of Genetically Engineered Cells Releasing Neurotrophic Factors
. Although these methods have been used primarily to express reporter genes like β-galactosidase (β-gal), they are general methods that can be used to express other genes, such as growth factors. Nerve growth factor (NGF) is the only growth factor that has been expressed
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