Recombinant Human beta-NGF/β-N

Recombinant Human beta-NGF/β-Nerve Growth Factor/β-NGF  (CI51)

 

产品说明(Description)

Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed. 

Accession #: P01138

Known as: Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH7.0.

 

质量控制（Quality Control） 

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:   Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

                       The ED50 for this effect is 0.04-0.4 ng/mL.

                       The ED50 for this effect is typically 0.2 ng/mL.（Novoprotein）
                       The ED50 for this effect is typically 1.06 ng/mL. (R)

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Gαq-mediated calcium dynamics and membrane tension modulate neurite plasticity[J]. Katherine M. Pearce. et al. Molecular Biology of the Cell. 2019.

SORT1 mutation resulting in sortilin deficiency and p75NTR upregulation in a family with essential tremor[J]. Sánchez E.  et al. ASN neuro. 2015.

 

 

FOR RESEARCH USE ONLY