IGF-1/IGF-I细胞因子,重组小鼠胰岛素样生长因子-I

重组小鼠胰岛素样生长因子-I（IGF-1）| His标签 | 重组蛋白 | E.coli表达

产品信息

• 产品名称：Mouse IGF-1 protein, His tag (Animal-Free)
• 货号：PRP1146
• 种属：小鼠 (Mus musculus)
• 蛋白来源：氨基酸序列源自 UniProt P05017 (Gly49–Ala118)
• 表达系统：大肠杆菌（E. coli）
• 标签：C-末端 6×His 标签
• 分子量（计算值）：8.61 kDa
• SDS-PAGE迁移：11–17 kDa（还原条件）
• 纯度：>98%（SDS-PAGE检测）
• 蛋白形式：冻干粉，溶于1X PBS (pH 8.0)，经0.2 µm过滤
• 内毒素水平：<0.1 EU/μg（LAL法检测）
• 生物活性：诱导MCF-7细胞增殖，ED₅₀ < 2 ng/mL；比活力 >5 × 10⁵ IU/mg
• 别名：Somatamedin C, IGF-IA
• 基因ID：Null
• 蛋白质ID：P05017

应用背景

胰岛素样生长因子-I（IGF-I）是介导生长激素代谢效应的关键因子，广泛参与细胞增殖、糖原合成、DNA复制及葡萄糖摄取调控。其在肝脏、脂肪组织、子宫内膜基质细胞及白细胞中高表达，亦可从血浆中分离。本品为重组小鼠IGF-I，适用于体外细胞培养体系中模拟生理状态下的IGF-I信号通路研究，尤其适用于需要无动物源成分的实验环境。

产品特性与优势

• 无动物源成分：生产过程不使用动物源性原料，降低外源污染风险，适用于高要求的细胞研究。
• 高纯度与低内毒素：纯度>98%，内毒素水平<0.1 EU/μg，满足对背景干扰敏感的细胞实验需求。
• 明确活性验证：活性通过标准MCF-7细胞增殖实验验证，确保批次间功能一致性。
• 稳定冻干形式：采用PBS缓冲体系冻干，便于长期保存与精确复溶。

使用说明

• 复溶建议：使用无菌ddH₂O复溶，浓度不低于100 μg/mL。复溶后于室温孵育≥20分钟，确保完全溶解。请勿振荡！建议轻柔颠倒混匀。
• 离心提示：开盖前请以3000 rpm离心5分钟，确保冻干粉完全沉于管底。
• 稀释建议：长期储存前，建议将工作液稀释于含0.1% BSA、10% FBS或5% HSA的缓冲液中，以增强稳定性。

储存与运输

• 冻干状态：–20°C保存，有效期12个月。
• 复溶后：2–8°C可稳定保存1周；如需长期保存，请分装后于–20°C或–80°C保存3–6个月。
• 运输条件：冰袋运输（蓝冰）
• 重要提示：避免反复冻融循环，每次使用后建议分装保存。

规格与价格

重要声明

本产品仅限科研用途，不可用于人类或临床诊断。所有推荐应用均基于现有文献与内部验证，不代表对任何专利或许可的授权。使用者须自行确认其研究行为符合相关知识产权法规，我方对因使用本产品引发的专利侵权或其他法律纠纷不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Insulin-like Growth Factor-I (IGF-1) | His Tag | Recombinant Protein | E. coli Expression

Product Information

• Product Name: Mouse IGF-1 protein, His tag (Animal-Free)
• Catalog Number: PRP1146
• Species: Mouse (Mus musculus)
• Protein Source: Amino acid sequence derived from UniProt P05017 (Gly49–Ala118)
• Expression System: Escherichia coli (E. coli)
• Tag: C-terminal 6×His tag
• Molecular Weight (Calculated): 8.61 kDa
• SDS-PAGE Migration: 11–17 kDa (Under reducing conditions)
• Purity: >98% (by SDS-PAGE analysis)
• Protein Form: Lyophilized powder, solubilized in 1X PBS (pH 8.0), filtered through 0.2 µm
• Endotoxin Level: <0.1 EU/µg (determined by LAL assay)
• Biological Activity: Induces proliferation of MCF-7 cells, ED₅₀ < 2 ng/mL; Specific activity >5 × 10⁵ IU/mg
• Aliases: Somatamedin C, IGF-IA
• Gene ID: Null
• Protein ID: P05017

Background

Insulin-like Growth Factor-I (IGF-I) is a key factor mediating the metabolic effects of growth hormone, widely involved in cell proliferation, glycogen synthesis, DNA replication, and regulation of glucose uptake. It is highly expressed in the liver, adipose tissue, endometrial stromal cells, and leukocytes, and can also be isolated from plasma. This product is recombinant mouse IGF-I, suitable for studying the IGF-I signaling pathway under physiological conditions in in vitro cell culture systems, particularly for experimental environments requiring animal-free components.

Features & Advantages

• Animal-Free: The production process does not use animal-derived raw materials, reducing the risk of exogenous contamination, suitable for high-demand cell research.
• High Purity & Low Endotoxin: Purity >98%, endotoxin level <0.1 EU/µg, meeting the needs of cell experiments sensitive to background interference.
• Clearly Validated Activity: Activity is verified by standard MCF-7 cell proliferation assay, ensuring functional consistency between batches.
• Stable Lyophilized Form: Lyophilized in PBS buffer system, facilitating long-term storage and accurate reconstitution.

Usage Instructions

• Reconstitution Recommendation: Reconstitute using sterile ddH₂O to a concentration not less than 100 µg/mL. After reconstitution, incubate at room temperature for ≥20 minutes to ensure complete dissolution. Do NOT vortex! Gentle inversion for mixing is recommended.
• Centrifugation Tip: Before opening, centrifuge the vial at 3000 rpm for 5 minutes to ensure the lyophilized powder is completely settled at the bottom of the tube.
• Dilution Recommendation: For long-term storage, it is recommended to dilute the working solution in a buffer containing 0.1% BSA, 10% FBS, or 5% HSA to enhance stability.

Storage & Shipping

• Lyophilized State: Store at –20°C, stable for 12 months.
• After Reconstitution: Stable for 1 week at 2–8°C; for long-term storage, aliquot and store at –20°C or –80°C for 3–6 months.
• Shipping Conditions: Shipped with ice packs (blue ice).
• Important Note: Avoid repeated freeze-thaw cycles. Aliquoting after each use is recommended.

Specifications & Pricing

Important Statement

This product is intended for research use only and is not for human or clinical diagnostic use. All recommended applications are based on existing literature and internal validation and do not represent authorization of any patent or license. Users must independently ensure that their research activities comply with relevant intellectual property laws and regulations. We assume no liability for patent infringement or other legal disputes arising from the use of this product.

Note: The above information may change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.