ARIA, NRG1, GGF, HGL, HRG, HRG

重组人神经调节蛋白1-β1 (NRG1-β1) 无标签冻干粉

货号: PRP1954

产品类型: 重组细胞因子

种属来源: 人源

产品概述

本产品为高纯度重组人神经调节蛋白1-β1 (Human NRG1 Beta1 Protein)，由大肠杆菌表达系统制备，无标签，以冻干粉形式提供。该蛋白在神经发育、神经传递和突触可塑性等基础细胞生物学研究中具有重要应用价值。

关键特性

• 高纯度: 经SDS-PAGE检测，纯度 > 99%
• 低内毒素: 每微克蛋白内毒素含量 < 1.0 EU (LAL法测定)
• 无标签: 保留蛋白天然活性，减少对实验的潜在干扰
• 生物活性验证: 经MCF-7细胞增殖实验测定，ED₅₀为0.6730 ng/mL

产品规格

技术参数

• 蛋白序列: 重组人NRG1-β1 (UniProt ID: Q02297, 氨基酸位置S20-K241)
• 分子量: 预测分子量为24.04 KD
• 蛋白长度: 220个氨基酸
• 表达宿主: 大肠杆菌
• 冻干缓冲液: 无菌20 mM Tris, 150 mM NaCl, pH 7.4

使用建议

开盖前请先离心。推荐使用配套复溶缓冲液将冻干粉复溶至浓度不低于100 µg/mL，后续可根据实验需求进行稀释。复溶后的蛋白可在2-8°C保存一周，稀释后分装的蛋白建议在含载体蛋白(如0.1% HSA或BSA)的缓冲液中于-20°C或-80°C保存3-6个月，避免反复冻融。

背景信息

神经调节蛋白1 (NRG1)是neuregulin家族四个成员之一，作用于EGFR家族受体。该生长因子最初被鉴定为一种44 kD糖蛋白，能够与NEU/ERBB2受体酪氨酸激酶相互作用并增加其酪氨酸残基磷酸化。NRG1作为神经营养因子，在神经发育、神经传递和突触可塑性中发挥重要作用。研究表明，NRG1的功能失调与癌症和精神分裂症等疾病的发生发展相关。

别名

ARIA, NRG1, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor-inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor

运输与保存

本品采用蓝冰冰袋运输。冻干粉可在-20°C下稳定保存1年。

注意事项

本产品仅限于研究使用，不适用于人类或临床诊断。使用前请查阅相关文献，确保应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Neuregulin‑1 β1 (NRG1‑β1) Tag‑free Lyophilized Powder

Product Code: PRP1954

Product Type: Recombinant Cytokine

Species Origin: Human

Product Overview

This product is a high‑purity recombinant human Neuregulin‑1 β1 protein produced in an Escherichia coli expression system. It is supplied as a tag‑free lyophilized powder. The protein is valuable for fundamental cell‑biological studies of neural development, neurotransmission, and synaptic plasticity.

Key Features

• High Purity: SDS‑PAGE analysis shows purity > 99%
• Low Endotoxin: < 1.0 EU per µg protein (LAL assay)
• Tag‑free: Preserves native activity and minimizes experimental interference
• Biological Activity Confirmation: MCF‑7 proliferation assay yields an ED₅₀ of 0.6730 ng/mL

Product Specifications

Technical Parameters

• Protein Sequence: Recombinant human NRG1‑β1 (UniProt ID: Q02297, amino‑acid positions S20‑K241)
• Molecular Weight: Predicted 24.04 kDa
• Protein Length: 220 amino acids
• Expression Host: E. coli
• Lyophilization Buffer: Sterile 20 mM Tris, 150 mM NaCl, pH 7.4

Usage Recommendations

Prior to opening, centrifuge the vial. Reconstitute the lyophilized powder with the supplied buffer to achieve a concentration of at least 100 µg/mL; subsequently dilute as required for your experiments. The reconstituted protein is stable at 2‑8 °C for up to one week. Aliquoted protein diluted in a buffer containing a carrier protein (e.g., 0.1 % HSA or BSA) should be stored at –20 °C or –80 °C for 3‑6 months, avoiding repeated freeze‑thaw cycles.

Background

Neuregulin‑1 (NRG1) is one of four members of the neuregulin family and signals through the EGFR family of receptors. Initially identified as a 44 kDa glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase and enhances its tyrosine phosphorylation, NRG1 acts as a neurotrophic factor playing crucial roles in neural development, neurotransmission, and synaptic plasticity. Dysregulation of NRG1 has been linked to oncogenesis and psychiatric disorders such as schizophrenia.

Synonyms

ARIA, NRG1, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor‑inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron‑derived factor

Shipping & Storage

The product is shipped on blue‑ice gel packs. The lyophilized powder remains stable for up to one year when stored at –20 °C.

Precautions

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Prior to use, consult the relevant literature to ensure that your intended application does not infringe any patents or licensing agreements.

Note: The above information may change as the product is updated or optimized; please contact Yakeyin for the most current details.