HMG-1, HMG1, HMG3, SBP-1, 重组蛋白

重组小鼠HMGB1蛋白 (His标签，无动物源) | 高纯度研究级细胞因子

货号：PRP1306

产品名称：Mouse HMGB1 Protein, His tag (Animal-Free)

产品描述

本产品为采用大肠杆菌表达系统制备的重组小鼠高迁移率族蛋白B1（HMGB1），C端带有6×His标签，全程无动物源成分。该蛋白来源于小鼠HMGB1 (Met1-Glu215)序列（UniProt ID: P63158），由215个氨基酸组成，预测分子量为25.56 kDa。经SDS-PAGE检测，纯度高于95%，内毒素含量低于0.1 EU/μg，适用于细胞培养及相关分子机制研究。

关键特性

• 高纯度： >95%，SDS-PAGE验证
• 低内毒素： <0.1 EU/μg (LAL法测定)
• 生物活性： 经测定，可在RAW264.7细胞中诱导TNF-α产生，ED₅0 <15 ng/mL
• 无动物源表达： 避免外源动物蛋白和病毒污染风险
• 标签类型： C端6×His标签，便于纯化与检测

产品形态与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌1×PBS（pH 7.4）。使用前请离心，推荐使用配套复溶缓冲液复溶至浓度不低于100 µg/mL，后续可根据实验需要进行稀释。

保存与运输条件

• 保存： 冻干粉可在-20℃以下干燥环境中长期保存；复溶后可在4℃保存2–7天，长期保存请分装并存于-20℃以下，建议添加载体蛋白（如0.1% BSA）。
• 运输： 蓝冰冰袋运输，保障产品稳定性。

背景信息

HMGB1（High Mobility Group Box 1）属于高迁移率族蛋白家族，是一种非组蛋白染色体蛋白。其结构包括两个HMG box结构域（A和B）和一个富含酸性氨基酸的C端区域。HMGB1在细胞应激、炎症及细胞死亡过程中发挥重要调控作用。研究表明，其半胱氨酸残基的氧化状态（如C23与C45间二硫键的形成）与NF-κB激活和细胞因子诱导密切相关。

应用领域

• 细胞因子与炎症机制研究
• 先天免疫与信号通路分析
• 细胞凋亡与坏死研究
• 蛋白质相互作用及功能验证实验

规格与价格

规格	价格（元）	货期
5 μg	588	现货，次日发货
20 μg	1498	现货，次日发货
100 μg	3998	请咨询
1 mg	请咨询	请咨询

注意：本品仅供科研使用，不适用于人体或临床诊断。使用前请查阅相关文献并了解可能涉及的专利许可问题。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse HMGB1 Protein (His Tag, Animal-Free) | High Purity Research Grade Cytokine

Catalog Number: PRP1306

Product Name: Mouse HMGB1 Protein, His tag (Animal-Free)

Product Description

This product is a recombinant mouse High Mobility Group Box 1 (HMGB1) protein prepared using an E. coli expression system, with a C-terminal 6×His tag and entirely free of animal-derived components. The protein is derived from the mouse HMGB1 (Met1-Glu215) sequence (UniProt ID: P63158), consists of 215 amino acids, and has a predicted molecular weight of 25.56 kDa. As determined by SDS-PAGE, the purity is greater than 95%, with endotoxin levels below 0.1 EU/μg. It is suitable for cell culture and related molecular mechanism studies.

Key Features

• High Purity: >95%, verified by SDS-PAGE
• Low Endotoxin: <0.1 EU/μg (determined by LAL assay)
• Biological Activity: Demonstrated ability to induce TNF-α production in RAW264.7 cells, with an ED₅₀ <15 ng/mL
• Animal-Free Expression: Avoids risks associated with extraneous animal proteins and viral contamination
• Tag Type: C-terminal 6×His tag, facilitating purification and detection

Product Form and Reconstitution Recommendations

This product is provided as a lyophilized powder, lyophilized from a sterile 1×PBS buffer (pH 7.4). Centrifuge briefly before use. It is recommended to reconstitute the powder to a concentration not less than 100 µg/mL using the provided reconstitution buffer, and it can be further diluted according to experimental needs.

Storage and Shipping Conditions

• Storage: The lyophilized powder can be stored long-term in a dry environment at -20°C or below. After reconstitution, it can be stored at 4°C for 2–7 days. For long-term storage, aliquot and store at -20°C or below, preferably with the addition of a carrier protein (e.g., 0.1% BSA).
• Shipping: Shipped with blue ice packs to ensure product stability.

Background Information

HMGB1 (High Mobility Group Box 1) belongs to the high mobility group box family of proteins and is a non-histone chromosomal protein. Its structure includes two HMG box domains (A and B) and an acidic C-terminal tail rich in acidic amino acids. HMGB1 plays important regulatory roles in cellular stress, inflammation, and cell death processes. Research indicates that the redox state of its cysteine residues (such as the formation of a disulfide bond between C23 and C45) is closely related to NF-κB activation and cytokine induction.

Applications

• Cytokine and inflammation mechanism studies
• Innate immunity and signaling pathway analysis
• Apoptosis and necrosis research
• Protein interaction and functional validation experiments

Specifications and Pricing

Specification	Price (CNY)	Availability
5 μg	588	In stock, ships next day
20 μg	1498	In stock, ships next day
100 μg	3998	Please inquire
1 mg	Please inquire	Please inquire

Note: This product is intended for research use only and is not suitable for human use or clinical diagnosis. Please review relevant literature and be aware of any potential patent licensing issues before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeen Company for specific details.