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- Solarbio
- P00077
- 北京
- 2025年07月25日
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￥520
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- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥21120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥14784.0 |
| 规格： | 50ug | 产品价格： | ￥4004.0 |
| 规格： | 10ug | 产品价格： | ￥1331.0 |
Recombinant Human High Mobility Group Protein B1 is produced by our Mammalian expression system and the target gene encoding Gly2-Glu215 is expressed with a 6His tag at the C-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | High Mobility Group Protein B1;High Mobility Group Protein 1;HMG-1;HMGB1;HMG1 |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | High mobility group protein B1 is a member of the HMGB family consisting of three members，HMGB1，HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus.As a nuclear protein，HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival.Meanwhile，it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte，T cell，dendritic cell activities in inflammatory responses. |
| 来源 | Human cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| 单位 | 支 |
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我们知道，PeproTech的所有细胞因子和蛋白均为冻干粉，这使得运输非常便捷，只要常温即可。而且，细胞因子和蛋白冻干粉非常稳定，在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解，然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键，因溶解不好会导致细胞因子或蛋白的失活，这也是很多用户在实际使用中经常遇到的问题。 那么，应该如何进行正确的溶解呢？ 下面我们以Recombinant Human IL-4 (重组人IL-4，产品编号：200-04)的说明书为例
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