Fibroblast Growth Factor 1, FG

重组人酸性成纤维细胞生长因子（FGF-1/aFGF）——细胞增殖与组织修复研究的核心工具

产品定位

作为非糖基化FGF家族成员，本品以大肠杆菌系统表达，提供高批次一致性的活性蛋白，专为细胞培养、类器官扩增、血管生成及创伤修复模型等研究场景设计。

关键参数速览

• 货号：PRP1001
• 分子信息：140 aa，15.5 kDa，无标签，人源序列（Phe16-Asp155，UniProt P05230）
• 活性验证：Balb/3T3 增殖实验，ED50 23.73 ng/mL
• 纯度：SDS-PAGE > 98 %
• 内毒素：LAL 法 ≤ 1.0 EU/µg

产品形式与复溶

冻干粉，缓冲体系 20 mM Tris，50 mM NaCl，pH 6.5。开盖前短时离心，推荐使用配套复溶液，终浓度≥100 µg/mL，可根据实验梯度稀释。

保存与运输

• 未溶解：−20 °C 以下干燥环境，可稳定 2 年
• 已溶解：4 °C 短期 2-7 天；长期需加 0.1 % HSA/BSA，分装后 −20 °C 以下避光保存，避免反复冻融
• 运输：常温干冰防护，次日现货发出

规格与询价

研究提示

FGF-1 被广泛报道参与血管生成、神经保护、皮肤与角膜损伤修复等过程。建议在三维共培养、基质胶插管或小鼠 Matrigel 栓实验中，与肝素或肝素类似物联用以提升稳定性。

法规声明

仅限科研使用，不可用于临床或诊断。用户需自行确认实验方案与下游应用是否涉及第三方专利或监管限制。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Acidic Fibroblast Growth Factor (FGF-1/aFGF) – Core Tool for Cell Proliferation and Tissue Repair Research

Product Positioning

As a non‑glycosylated member of the FGF family, this product is expressed in an E. coli system, providing high batch‑to‑batch consistency of active protein, specifically designed for research scenarios such as cell culture, organoid expansion, angiogenesis, and wound‑healing models.

Key Parameter Overview

• Catalog No.: PRP1001
• Molecular Information: 140 aa, 15.5 kDa, tag‑free, human sequence (Phe16‑Asp155, UniProt P05230)
• Activity Confirmation: Balb/3T3 proliferation assay, ED50 23.73 ng/mL
• Purity: SDS‑PAGE > 98 %
• Endotoxin: LAL assay ≤ 1.0 EU/µg

Product Form and Reconstitution

Lyophilized powder, buffer system 20 mM Tris, 50 mM NaCl, pH 6.5. Prior to opening, perform a brief centrifugation. Recommended to use the supplied reconstitution solution; final concentration ≥ 100 µg/mL, with subsequent serial dilutions as required.

Storage and Shipping

• Unreconstituted: Dry environment at ≤ −20 °C, stable for up to 2 years
• Reconstituted: Short‑term 4 °C for 2–7 days; for long‑term storage add 0.1 % HSA/BSA, aliquot and keep protected from light at ≤ −20 °C, avoid repeated freeze‑thaw cycles
• Shipping: Ambient temperature with dry‑ice protection; next‑day dispatch from stock

Specifications and Pricing

Research Tips

FGF‑1 is widely reported to participate in angiogenesis, neuroprotection, and skin or corneal injury repair. It is advisable to combine it with heparin or heparin‑mimetic compounds in 3D co‑culture, matrix‑gel inserts, or mouse Matrigel plug assays to enhance stability.

Regulatory Statement

For research use only; not for clinical or diagnostic applications. Users must independently verify whether their experimental protocols or downstream uses involve third‑party patents or regulatory restrictions.

Note: The above information may change due to product optimization or upgrades. For the most current details, please contact Yakeyin Company.