fibroblast growth factor acidi

产品概述

产品名称：重组大鼠酸性成纤维细胞生长因子（FGF‑1）
英文名称：Rat FGF‑1 Protein
货号：PRP1239
别名：fibroblast growth factor acidic, HBGF‑1, ECGF‑beta, FGF1, FGF acidic, aFGF, 成纤维生长因子‑1（大鼠）

核心属性

• 蛋白质长度：140 个氨基酸（氨基酸序列来源：大鼠FGF1，UniProt P61149，Phe16‑Asp155）
• 预测分子量：15.9 kDa
• 表达宿主：Escherichia coli
• 纯度：> 98 %，通过SDS‑PAGE检测确认
• 内毒素水平：< 0.1 EU/µg（LAL法）
• 活性状态：Testing in Progress（实验室活性验证进行中）

应用领域

本产品定位为细胞因子核心产品，适用于细胞培养及相关研究。

• 细胞增殖与分化研究
• 血管生成（angiogenesis）模型
• 胚胎发育与组织再生研究
• 信号通路（FGFR）功能分析

技术规格

产品形态与配套缓冲液

• 形态：冻干粉（无菌PBS，pH 6.5）
• 建议使用本公司提供的Reconstitution Buffer进行复溶，复溶浓度不低于 103 µg/mL，后续可根据实验需求稀释。

使用前处理

• 开盖后请先进行离心（≥ 10,000 g，5 min），去除可能的颗粒。
• 复溶后若不立即使用，建议在4 °C保存 2–7 天；长期保存请回存至‑20 °C。
• 为降低反复冻融对活性的影响，可在复溶液中加入 0.1 % HSA 或 BSA 作为载体蛋白，并分装保存。

储存与运输

• 冻干状态：‑20 °C以下干燥环境
• 溶解后：4 °C短期（≤ 7 天），‑20 °C长期
• 运输方式：蓝冰（冷链）包装，确保全程低温

检测与质量控制

• 分子量检测：SDS‑PAGE（确认 15.9 kDa）
• 纯度评估：≥ 98 %（SDS‑PAGE）
• 内毒素检测：LAL 法，< 0.1 EU/µg
• 活性检测：正在进行中（结果将随批次更新）

合规与风险提示

本产品仅限用于 科研目的，不适用于人类或临床诊断。使用过程中请遵守当地知识产权及生物安全法规。若因使用本产品导致的专利侵权或其他合规问题，供货方不承担责任。

参考信息

• 基因ID：16171
• 蛋白质ID：P61149（UniProtKB）查看序列
• FGF1 为已知 23 种 FGF 家族成员之一，参与胚胎发育、后期生长及组织再生等多种生理过程，能够通过所有已鉴定的 FGF 受体进行信号传导。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Recombinant Rat Acidic Fibroblast Growth Factor (FGF‑1)
English Name: Rat FGF‑1 Protein
Catalog No.: PRP1239
Aliases: fibroblast growth factor acidic, HBGF‑1, ECGF‑beta, FGF1, FGF acidic, aFGF, Fibroblast Growth Factor‑1 (Rat)

Key Attributes

• Protein length: 140 amino acids (sequence derived from rat FGF1, UniProt P61149, Phe16‑Asp155)
• Predicted molecular weight: 15.9 kDa
• Expression host: Escherichia coli
• Purity: > 98 %, confirmed by SDS‑PAGE
• Endotoxin level: < 0.1 EU/µg (LAL assay)
• Activity status: Testing in Progress (in‑house activity validation ongoing)

Application Areas

This product is positioned as a core cytokine reagent, suitable for cell culture and related studies.

• Cell proliferation and differentiation studies
• Angiogenesis models
• Embryonic development and tissue regeneration research
• FGFR signaling pathway functional analysis

Technical Specifications

Product Formulation and Buffer

• Form: Lyophilized powder (sterile PBS, pH 6.5)
• It is recommended to reconstitute with the company‑supplied Reconstitution Buffer to a concentration not less than 103 µg/mL; thereafter it can be diluted according to experimental needs.

Pre‑Use Handling

• After opening, centrifuge (≥ 10,000 g, 5 min) to remove any particulates.
• If not used immediately after reconstitution, store at 4 °C for 2–7 days; for long‑term storage, keep at ‑20 °C.
• To mitigate activity loss from repeated freeze‑thaw cycles, add 0.1 % HSA or BSA as a carrier protein to the reconstituted solution and aliquot for storage.

Storage and Shipping

• Lyophilized state: dry environment below ‑20 °C
• Post‑reconstitution: short‑term at 4 °C (≤ 7 days), long‑term at ‑20 °C
• Shipping method: blue‑ice (cold‑chain) packaging to ensure continuous low temperature

Testing and Quality Control

• Molecular weight analysis: SDS‑PAGE (confirming 15.9 kDa)
• Purity assessment: ≥ 98 % (SDS‑PAGE)
• Endotoxin testing: LAL assay, < 0.1 EU/µg
• Activity assay: ongoing (results will be updated with each batch)

Regulatory and Risk Disclaimer

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Users must comply with local intellectual‑property and biosafety regulations. The supplier assumes no liability for patent infringements or other compliance issues arising from the use of this product.

Reference Information

• Gene ID: 16171
• Protein ID: P61149 (UniProtKB) View sequence
• FGF1 is one of the 23 known members of the FGF family, involved in embryonic development, postnatal growth, and tissue regeneration, and can signal through all identified FGF receptors.

Note: The above information may change due to product optimization or upgrades; please contact Yakoin Company for the most current details.