FGF1/aFGF细胞因子,重组人酸性成纤维细胞生长因子,重

重组人酸性成纤维细胞生长因子 (FGF1/aFGF)

货号：PRP1001 | 产品类型：无标签，高纯度细胞因子

产品概述

重组人FGF1/aFGF蛋白是一种高活性、无标签的细胞因子，采用大肠杆菌表达系统重组表达。该蛋白来源于人源FGF1基因（P05230），由140个氨基酸组成，预测分子量为15.5 kDa，纯度经SDS-PAGE检测大于98%。本产品在细胞培养、组织工程及相关信号通路研究中具有广泛的应用价值。

关键特性

• 高生物活性：经Balb/3T3细胞增殖实验验证，ED₅₀为23.73 ng/mL。
• 超高纯度：纯度 > 98%，确保实验结果的可重复性与可靠性。
• 低内毒素水平：采用LAL法检测，每1 μg蛋白质中EU含量 < 1.0，适用于对内毒素敏感的细胞实验。
• 无动物源成分：大肠杆菌表达系统，避免动物源病原体污染风险。

产品规格与价格

使用说明

复溶建议：推荐使用配套复溶缓冲液（无菌20 mM Tris，50 mM NaCl，pH 6.5），复溶浓度不低于100 µg/mL。复溶后可根据实验需求进一步稀释。

保存条件：冻干粉应保存于-20℃以下干燥环境。复溶后可在4℃保存2-7天，长期保存请置于-20℃以下，建议添加0.1% HSA或BSA作为载体蛋白，分装避免反复冻融。

背景信息

FGF1（酸性成纤维细胞生长因子）是一种多功能生长因子，参与胚胎发育、血管生成、伤口修复及细胞增殖等关键生理过程。其在组织工程、干细胞研究与肿瘤生物学中具有重要的科研价值。

注意：本产品仅供科研使用，不适用于临床诊断或人体应用。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Acidic Fibroblast Growth Factor (FGF1/aFGF)

Catalog No.: PRP1001 | Product Type: Tag‑free, high‑purity cytokine

Product Overview

The recombinant human FGF1/aFGF protein is a highly active, tag‑free cytokine expressed using an Escherichia coli expression system. The protein is derived from the human FGF1 gene (P05230), comprises 140 amino acids, with a predicted molecular weight of 15.5 kDa, and exhibits purity > 98% as assessed by SDS‑PAGE. This product has broad utility in cell culture, tissue engineering, and related signaling pathway studies.

Key Features

• High biological activity: Validated in Balb/3T3 cell proliferation assays with an ED₅₀ of 23.73 ng/mL.
• Ultra‑high purity: Purity > 98%, ensuring reproducibility and reliability of experimental results.
• Low endotoxin level: Determined by LAL assay, endotoxin units (EU) < 1.0 per µg of protein, suitable for endotoxin‑sensitive cell assays.
• Animal‑origin free: Produced in an E. coli expression system, eliminating the risk of animal‑derived pathogen contamination.

Product Specifications and Pricing

Usage Instructions

Reconstitution recommendation: It is recommended to use the supplied reconstitution buffer (sterile 20 mM Tris, 50 mM NaCl, pH 6.5) to achieve a concentration of ≥ 100 µg/mL. The solution can be further diluted as required for assays.

Storage conditions: Lyophilized powder should be stored in a dry environment at ≤ ‑20 °C. After reconstitution, the solution may be kept at 4 °C for 2–7 days; for long‑term storage, keep at ≤ ‑20 °C, preferably adding 0.1 % HSA or BSA as a carrier protein and aliquoting to avoid repeated freeze‑thaw cycles.

Background Information

FGF1 (acidic fibroblast growth factor) is a multifunctional growth factor involved in embryonic development, angiogenesis, wound healing, and cell proliferation. It holds significant research value in tissue engineering, stem cell studies, and cancer biology.

Note: This product is for research use only and is not intended for clinical diagnosis or human application.

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakein Co. for the most current details.