- ￥588 - 3998
- 亚科因(Abbkine)
- PRP1069-5
- 湖北武汉
- 2026年01月04日
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- 库存：
999
- 供应商：
亚科因(Abbkine)
- 英文名：
Human TGF-β3 protein, His tag (Animal-Free)
- 规格：
5 μg/20 μg/100 μg/1 mg
| 规格： | 5 μg | 产品价格： | ￥588.0 |
|---|---|---|---|
| 规格： | 20 μg | 产品价格： | ￥1498.0 |
| 规格： | 100 μg | 产品价格： | ￥3998.0 |
| 规格： | 1 mg | 产品价格： | 询价 |
重组人转化生长因子-β3 (TGF-β3) 蛋白，His标签 (无动物源)
货号：PRP1069
本产品为高纯度、高活性、无动物源表达的重组人源转化生长因子-β3 (TGF-β3) 蛋白，专为严格的细胞培养及相关生物医学研究设计。产品采用先进的重组技术在大肠杆菌中表达，确保批次间的高度一致性和极低的内毒素水平，为您的关键实验提供可靠保障。
产品概述
转化生长因子-β3 (TGF-β3) 是 TGF-β 超家族的重要成员，在调节细胞生长、分化、凋亡以及胚胎发育（尤其是心脏形态发生和上皮-间质转化（EMT））等过程中扮演着核心角色。本品为 Ala301-Ser412 氨基酸片段的重组蛋白，C末端带有6×His标签，便于检测与纯化。
关键特性与质量保证
- 高纯度：经SDS-PAGE分析，纯度 > 98%。
- 高比活性：特异性活性 > 2 × 10⁷ IU/mg。通过抑制IL-4诱导的HT-2细胞增殖实验测定，其ED50 < 50 pg/mL。
- 低内毒素：采用LAL法检测，每1μg蛋白质中的内毒素（EU）含量 < 0.1，极大降低了由内毒素引起的实验干扰。
- 无动物源成分：整个生产过程（包括表达宿主）均无动物源成分，降低了病毒和病原体污染的风险，适用于对安全性要求极高的研究。
- 准确的分子量：计算分子量为13.66 kDa，在还原性SDS-PAGE中迁移为约13 kDa。
产品形式与复溶
本品为冻干粉形式，由含0.01% sarkosyl的1X PBS (pH 8.0)溶液经0.2 µm过滤后冻干而成。
复溶建议：
打开前请以3000 rpm离心5分钟。建议使用无菌ddH2O复溶，使终浓度不低于100 μg/mL。在室温下静置孵育至少20分钟以确保充分溶解。请注意：切勿振荡或涡旋，以免破坏蛋白活性。复溶后的储备液可根据实验需求，在含有稳定剂（如0.1% BSA、10% FBS、5% HSA）的缓冲液中进行稀释。
保存与运输
- 保存条件：冻干粉可在-20°C下稳定保存1年。复溶后于2-8°C可保存1周。若在含稳定剂的缓冲液中稀释并分装，于-20°C或-80°C下可保存3-6个月。请避免反复冻融。
- 运输条件：全程采用蓝冰（冰袋）运输，确保产品在运输过程中的稳定性。
规格与价格（现货供应）
| 规格 | 价格（人民币） | 货期 |
|---|---|---|
| 5 μg | 588 | 现货，次日发货 |
| 20 μg | 1498 | 现货，次日发货 |
| 100 μg | 3998 | 请咨询 |
| 1 mg | 请咨询 | 请咨询 |
注意事项
警告：本品仅用于科学研究，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。对于因使用本产品可能导致的专利侵权或其他违规行为，我方不承担任何责任。
背景信息
TGF-β3（基因别名：ARVD, ARVD1, LDS5, RNHF, TGFB3）是一种由412个氨基酸组成的多功能细胞因子，其氨基酸序列与TGF-β1和TGF-β2分别有86%和91%的相似性。它在抑制DNA合成、调控细胞增殖、介导心脏发育中的EMT过程以及诱导乳腺上皮细胞凋亡等方面具有重要作用。
UniProt 登录号： P100600
注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Recombinant Human Transforming Growth Factor‑β3 (TGF‑β3) Protein, His‑Tag (Animal‑Component‑Free)
Catalog No.: PRP1069
This product is a high‑purity, high‑activity, animal‑component‑free expressed recombinant human Transforming Growth Factor‑β3 (TGF‑β3) protein, specifically designed for stringent cell culture and related biomedical research. The protein is produced using advanced recombinant technology in Escherichia coli*, ensuring high batch‑to‑batch consistency and extremely low endotoxin levels, providing reliable support for critical experiments.
Product Overview
Transforming Growth Factor‑β3 (TGF‑β3) is a key member of the TGF‑β superfamily, playing central roles in regulating cell growth, differentiation, apoptosis, and embryonic development (especially cardiac morphogenesis and epithelial‑mesenchymal transition (EMT)). This product comprises the Ala301‑Ser412 peptide fragment, with a C‑terminal 6×His tag to facilitate detection and purification.
Key Attributes and Quality Assurance
- High Purity: >98% purity as assessed by SDS‑PAGE.
- High Specific Activity: >2 × 10⁷ IU/mg. Determined by inhibition of IL‑4‑induced HT‑2 cell proliferation, ED₅₀ < 50 pg/mL.
- Low Endotoxin: Measured by LAL assay, endotoxin ≤0.1 EU per µg protein, markedly reducing endotoxin‑related assay interference.
- Animal‑Component‑Free: The entire manufacturing process (including expression host) is free of animal‑derived materials, minimizing risk of viral and pathogen contamination; suitable for highly safety‑critical research.
- Accurate Molecular Weight: Theoretical MW is 13.66 kDa; migrates at ~13 kDa under reducing SDS‑PAGE.
Product Form and Reconstitution
The product is supplied as a lyophilized powder, prepared by freeze‑drying a 1X PBS (pH 8.0) solution containing 0.01% sarkosyl that has been filtered through a 0.2 µm membrane.
Reconstitution Recommendation:
Prior to opening, centrifuge at 3000 rpm for 5 minutes. Reconstitute with sterile ddH2O to a final concentration of ≥100 µg/mL. Incubate at room temperature for at least 20 minutes to ensure complete dissolution. Note: Do not vortex or swirl to avoid loss of activity. The reconstituted stock can be diluted in buffer containing stabilizers (e.g., 0.1% BSA, 10% FBS, 5% HSA) as required for experiments.
Storage and Shipping
- Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year. Reconstituted material can be stored at 2–8 °C for up to 1 week. When diluted and aliquoted in buffer containing stabilizers, it can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.
- Shipping Conditions: Transported on dry ice (gel packs) throughout transit to ensure product stability.
Specifications and Pricing (In‑Stock)
| Specification | Price (CNY) | Lead Time |
|---|---|---|
| 5 µg | 588 | In stock, next‑day dispatch |
| 20 µg | 1498 | In stock, next‑day dispatch |
| 100 µg | 3998 | Please inquire |
| 1 mg | Please inquire | Please inquire |
Precautions
Warning: This product is intended for scientific research use only and is not suitable for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements. We assume no liability for any patent infringement or other violations arising from the use of this product.
Background Information
TGF‑β3 (gene aliases: ARVD, ARVD1, LDS5, RNHF, TGFB3) is a multifunctional cytokine composed of 412 amino acids, sharing 86% and 91% sequence similarity with TGF‑β1 and TGF‑β2, respectively. It plays important roles in inhibiting DNA synthesis, regulating cell proliferation, mediating EMT during cardiac development, and inducing apoptosis in mammary epithelial cells.
UniProt Accession: P100600
Note: The information above may change due to product optimization or upgrades; please contact Yakein Co. for the most current details.
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