CXCL7 重组人CXC趋化因子配体7 人源重组蛋白 细胞因

Human CXCL7 Protein, His tag (Animal-Free)

产品概述

本品为重组人源CXC趋化因子配体7（CXCL7）蛋白，采用无动物源表达系统，带有His标签，适用于细胞培养及相关科学研究。货号：PRP1105。

产品特性

• 序列来源：人源CXCL7 (Ala59-Asp128) (UniProt: P02775)
• 表达宿主：大肠杆菌表达系统，无动物源成分
• 蛋白长度：70个氨基酸，预测分子量为8.43 kDa
• 纯度：经SDS-PAGE检测，纯度 > 98%
• 内毒素水平：LAL法检测，每微克蛋白内毒素含量 < 0.1 EU
• 活性：通过趋化BaF3细胞（转染人CXCR2）测定，ED₅₀ < 0.5 ng/mL

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌PBS，pH 7.4。推荐使用配套复溶缓冲液进行复溶，复溶浓度建议不低于100 µg/mL。复溶后可根据实验需求进一步稀释。

保存与运输

• 保存条件：冻干粉可在-20°C下稳定保存1年；复溶后于2-8°C可保存1周；稀释后分装保存于-20°C或-80°C可稳定3-6个月，避免反复冻融。
• 运输条件：冰袋运输（蓝冰）

背景信息

CXCL7（Pro-Platelet basic protein, PPBP）属于CXC趋化因子家族，是一个由70个氨基酸组成、分子量约7.7 kDa的蛋白质。它主要在活化的血小板中表达，通过与CXCR2受体相互作用，在血管损伤过程中参与葡萄糖代谢、有丝分裂和中性粒细胞招募等生理过程。

应用提示

本品适用于细胞趋化实验、受体结合研究、信号通路分析等科研用途，建议在复溶后根据具体实验体系优化使用浓度。

规格与价格

注意事项

使用前请离心。本品仅供科研使用，不适用于人体或临床诊断。用户应自行确保使用方式不侵犯专利或许可协议。

基因ID：5473 | 别名：NAP-2, PPBP, CTAP-III, PBP

蛋白质数据库链接：UniProt: P02775

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human CXCL7 Protein, His tag (Animal-Free)

Product Overview

This product is a recombinant human CXC chemokine ligand 7 (CXCL7) protein expressed in an animal‑free system, bearing a His tag, suitable for cell culture and related scientific research. Catalog No.: PRP1105.

Product Features

• Sequence source: Human CXCL7 (Ala59‑Asp128) (UniProt: P02775)
• Expression host: Escherichia coli expression system, animal‑free components
• Protein length: 70 amino acids, predicted molecular weight 8.43 kDa
• Purity: > 98 % by SDS‑PAGE analysis
• Endotoxin level: LAL assay, < 0.1 EU per µg protein
• Activity: Chemotaxis assay using BaF3 cells transfected with human CXCR2, ED₅₀ < 0.5 ng/mL

Formulation and Reconstitution Recommendation

The product is supplied as a lyophilized powder; the lyophilization buffer is sterile PBS, pH 7.4. Reconstitution with the provided buffer is recommended, with a minimum concentration of 100 µg/mL. The stock solution can be further diluted according to experimental requirements.

Storage and Shipping

• Storage conditions: Lyophilized powder stable at –20 °C for 1 year; reconstituted solution stable at 2‑8 °C for 1 week; aliquoted diluted samples stable at –20 °C or –80 °C for 3‑6 months, avoid repeated freeze‑thaw cycles.
• Shipping conditions: Transport on ice packs (blue ice)

Background Information

CXCL7 (Pro‑Platelet basic protein, PPBP) belongs to the CXC chemokine family and is a 70‑amino‑acid protein with an approximate molecular weight of 7.7 kDa. It is primarily expressed in activated platelets and, through interaction with the CXCR2 receptor, participates in physiological processes such as glucose metabolism, mitosis, and neutrophil recruitment during vascular injury.

Application Notes

The product is suitable for chemotaxis assays, receptor‑binding studies, signaling pathway analyses, and other research applications. After reconstitution, optimize the working concentration according to the specific experimental system.

Specification and Pricing

Precautions

Centrifuge before use. This product is for research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that their use does not infringe any patents or licensing agreements.

Gene ID: 5473 | Aliases: NAP‑2, PPBP, CTAP‑III, PBP

Protein database link: UniProt: P02775

Note: The above information may change due to product optimization or upgrades. Please contact Yakeyin for the most current details.