重组人白介素-33，His-标签（无动物源）（IL-33）

重组人白介素-33（无动物源）His标签蛋白

产品核心参数

• 产品货号：PRP1091
• 蛋白类型：动物源游离表达体系制备
• 活性验证：ST2L结合ED₅₀ <54 ng/mL | D10.G4.1细胞增殖ED₅₀ <0.1 ng/mL
• 比活性：>4×10⁷ IU/mg

关键质量指标

• 纯度：SDS-PAGE检测＞98%
• 内毒素：<0.1 EU/μg（LAL法）
• 分子量：18.93 kDa（理论值）| 19 kDa（SDS-PAGE还原条件）
• 表达系统：大肠杆菌高效表达体系

产品规格与交付

专业应用指导

• 复溶建议：使用配套缓冲液（推荐浓度≥100 μg/mL）
• 保存方案：
  • 冻干粉：-20°C保存12个月
  • 复溶后：2-8°C保存7天
  • 稀释后：-20°C/-80°C保存3-6个月（需添加稳定剂）
• 运输保障：全程蓝冰冷链运输

分子功能特性

该重组蛋白包含人源IL-33功能域（Ser112-Thr270，UniProt ID：Q9HBE4），C端融合6×His标签。通过激活ST2L受体调控肥大细胞活化，介导IL-5/IL-13分泌，在2型免疫应答及过敏性炎症研究中具有重要应用价值。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-33 (Animal-Free) His-Tagged Protein

Product Core Parameters

• Product Code:PRP1091
• Protein Type:Prepared in Animal-Free Expression System
• Activity Validation:ST2L Binding ED₅₀ <54 ng/mL | D10.G4.1 Cell Proliferation ED₅₀ <0.1 ng/mL
• Specific Activity:>4×10⁷ IU/mg

Key Quality Indicators

• Purity:>98% by SDS-PAGE
• Endotoxin:<0.1 EU/μg (LAL Method)
• Molecular Weight:18.93 kDa (Theoretical) | 19 kDa (SDS-PAGE under Reducing Conditions)
• Expression System:High-Efficiency E. coli Expression System

Product Specifications and Delivery

Professional Application Guidance

• Reconstitution Recommendation:Use the provided buffer (Recommended concentration ≥100 μg/mL)
• Storage Solution:
  • Lyophilized Powder: Store at -20°C for 12 months
  • Reconstituted: Store at 2-8°C for 7 days
  • Diluted: Store at -20°C/-80°C for 3-6 months (Stabilizer Required)
• Transportation Assurance:Blue Ice Cold Chain Transportation

Molecular Functional Characteristics

This recombinant protein contains the human IL-33 functional domain (Ser112-Thr270, UniProt ID: Q9HBE4), with a C-terminal 6×His tag. It regulates mast cell activation by activating the ST2L receptor, mediates IL-5/IL-13 secretion, and has significant application value in type 2 immune response and allergic inflammation research.

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine Company.