CXCL4 重组人CXC趋化因子配体4 人源重组蛋白 细胞因

Human CXCL4 Protein, His tag (Animal-Free) - 重组人CXC趋化因子配体4 人源重组蛋白

货号: PRP1102

产品类型: 细胞因子

种属: 人源

表达宿主: 大肠杆菌表达系统

蛋白长度: 70个氨基酸，预测分子量为8.58 kDa

产品描述

重组人CXCL4蛋白是一种高纯度、动物源游离（Animal-Free）的重组蛋白，带有His标签，氨基酸序列来源于人源CXCL4 (Glu32-Ser101) (UniProt ID: P02776)。该蛋白通过大肠杆菌表达，经SDS-PAGE验证纯度大于98%，适用于细胞培养及相关研究应用。

关键特性与优势

• 高纯度: SDS-PAGE检测纯度 >98%
• 低内毒素: 每微克蛋白内毒素含量 <0.1 EU (LAL法测定)
• 生物活性验证: 经功能试验验证，可抑制人FGF-2诱导的HUVEC细胞增殖，ED₅₀ <5 μg/mL
• 无动物源成分: 生产过程中无动物源成分，降低外源因子风险

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌PBS (pH 7.4)。使用前请先离心，推荐使用配套复溶缓冲液（Reconstitution Buffer）复溶至浓度不低于100 µg/mL，后续可根据实验需求进一步稀释。

保存与运输条件

保存条件: 冻干粉在-20°C下可稳定保存1年；复溶后于2-8°C可保存1周；在含载体蛋白或稳定剂（如0.1% BSA、10% FBS、5% HSA）的缓冲液中稀释后，分装并于-20°C或-80°C下可保存3-6个月，避免反复冻融。

运输条件: 冰袋运输（蓝冰）

背景信息

CXCL4（又称Platelet Factor-4, PF4）属于CXC趋化因子家族，是一种由活化血小板产生的8 kDa蛋白质，在免疫应答、细胞增殖抑制、血小板聚集和伤口修复等生理过程中发挥重要作用。此外，CXCL4还能抑制造血过程。

基因ID: 5196 | 别名: Platelet Factor-4, PF-4, Oncostatin A, Ironplact

更多蛋白序列信息可参考: UniProt链接

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	请咨询
100 μg	3969	请咨询
1 mg	请咨询	请咨询

注意事项

警告: 本产品仅供研究使用，不适用于人类或临床诊断。使用前请阅读产品说明书，并遵守相关法律法规。对于因使用本产品可能涉及的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human CXCL4 Protein, His tag (Animal-Free) - Recombinant Human CXC Chemokine Ligand 4 Protein

Catalog Number: PRP1102

Product Type: Cytokine

Species: Human

Expression Host: E. coli Expression System

Protein Length: 70 amino acids, Predicted Molecular Weight: 8.58 kDa

Product Description

Recombinant Human CXCL4 Protein is a high-purity, animal-free recombinant protein with a His tag, derived from the human CXCL4 amino acid sequence (Glu32-Ser101) (UniProt ID: P02776). This protein is expressed in E. coli and exhibits >98% purity as verified by SDS-PAGE. It is suitable for cell culture and related research applications.

Key Features & Benefits

• High Purity: >98% purity as determined by SDS-PAGE analysis.
• Low Endotoxin: Endotoxin level is <0.1 EU per µg of protein (determined by LAL method).
• Bioactivity Validated: Functionally tested for its ability to inhibit human FGF-2-induced HUVEC proliferation, with an ED₅₀ <5 µg/mL.
• Animal-Free: Manufactured without animal-derived components, reducing the risk of adventitious agents.

Product Form & Reconstitution

This product is supplied as a lyophilized powder from a sterile PBS buffer (pH 7.4). Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized protein to a concentration of not less than 100 µg/mL using the provided reconstitution buffer. The reconstituted solution can be further diluted in other aqueous buffers as required for experimental applications.

Storage & Shipping

Storage: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, the protein can be stored at 2-8°C for up to 1 week. For long-term storage, aliquot the reconstituted solution and store at -20°C or -80°C. When diluted in a carrier protein-containing buffer (e.g., 0.1% BSA, 10% FBS, 5% HSA), the protein is stable for 3-6 months at -20°C or -80°C. Avoid repeated freeze-thaw cycles.

Shipping: Shipped on blue ice.

Background

CXCL4 (also known as Platelet Factor-4, PF4) is a member of the CXC chemokine family. It is an 8 kDa protein produced by activated platelets and plays significant roles in immune response, inhibition of cell proliferation, platelet aggregation, and wound repair. Additionally, CXCL4 can suppress hematopoiesis.

Gene ID: 5196 | Aliases: Platelet Factor-4, PF-4, Oncostatin A, Ironplact

For more protein sequence information, please refer to: UniProt Link

Specifications & Pricing

Specification	Price (RMB)	Lead Time
5 µg	588	In Stock (Ships Next Day)
20 µg	1498	Please Inquire
100 µg	3969	Please Inquire
1 mg	Please Inquire	Please Inquire

Notice

Warning: This product is for research use only and is not intended for human, therapeutic, or diagnostic use. Please read the product datasheet carefully before use and comply with all applicable laws and regulations. The company assumes no responsibility for patent infringement or any other violations that may result from the use of this product.

Note: The above information is subject to change due to product optimization and updates. For the most current details, please contact Yakonin Company.