TGF-α细胞因子,重组人转化生长因子α,重组蛋白,E.co

Human TGF‑α 重组蛋白 (His‑tag, Animal‑Free) – PRP1004

产品概述

本产品为来源于人源 TGF‑α（Val40‑Ala89）片段的重组蛋白，采用 E.coli 表达体系生产，并在蛋白 C 端引入 6×His 标签，便于纯化与后续分析。冻干形式提供，适用于细胞培养、信号通路研究以及癌症生物学等多种细胞类实验。

应用领域

• 细胞类 → 细胞培养
• 核心产品 → 细胞因子及蛋白
• 产品类型 → 细胞因子

技术参数

规格与报价

产品优势

• 高纯度 (> 98 %)，经 SDS‑PAGE 确认。
• 低内毒素 (< 0.1 EU/µg) 适合敏感细胞系的长期培养。
• 活性依赖于 EGFR 结合，ED₅₀ < 0.2 ng/mL，满足高效增殖需求。
• His 标签简化后续层析纯化与检测。
• 动物来源自由，适用于对动物成分有严格要求的实验体系。

使用指南

1. 使用本产品配套的 Reconstitution Buffer（无菌 PBS pH 8.0）复溶，建议终浓度 ≥ 100 µg/mL。
2. 复溶前请先离心去除可能的颗粒。
3. 根据实验需要，可在含 0.1 % BSA、10 % FBS 或 5 % HSA 的缓冲液中进一步稀释。
4. 避免反复冻融；建议一次性配制所需用量。

存储与稳定性

冻干粉建议保存在 ‑20 °C 冰箱中，保质期 1 年。复溶后请在 2‑8 °C 条件下使用，最长不超过 1 周。如需长期保存，建议在含载体蛋白或稳定剂的缓冲液中分装后置于 ‑20 °C 或 ‑80 °C，最多可保存 3‑6 个月。

运输 & 交付

产品采用蓝冰（冷链）方式运输，确保全程温度受控。现货（20 µg）可实现次日发货，其他规格请提前联系以确认交期。

合规声明

本产品仅限科研用途，不适用于人类临床或诊断。使用本产品进行的任何专利相关或许可证受限的研究，责任自负。我们不对因使用本产品而产生的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human TGF-α Recombinant Protein (His-tag, Animal-Free) – PRP1004

Product Overview

This product is a recombinant protein derived from the human TGF-α (Val40-Ala89) fragment, produced using an E.coli expression system. A 6×His tag is introduced at the C-terminus to facilitate purification and subsequent analysis. It is provided in lyophilized form and is suitable for various cell-based experiments such as cell culture, signaling pathway research, and cancer biology.

Application Fields

• Cell-based → Cell Culture
• Core Products → Cytokines & Proteins
• Product Type → Cytokine

Technical Parameters

Specifications & Pricing

Product Advantages

• High purity (> 98%), confirmed by SDS-PAGE.
• Low endotoxin (< 0.1 EU/µg) suitable for long-term culture of sensitive cell lines.
• Activity dependent on EGFR binding, ED₅₀ < 0.2 ng/mL, meeting high-efficiency proliferation demands.
• His tag simplifies subsequent chromatographic purification and detection.
• Animal-origin free, suitable for experimental systems with strict requirements regarding animal-derived components.

Usage Guide

1. Reconstitute using the provided Reconstitution Buffer (sterile PBS, pH 8.0), recommended final concentration ≥ 100 µg/mL.
2. Centrifuge before reconstitution to remove any potential particulates.
3. Can be further diluted in buffers containing 0.1% BSA, 10% FBS, or 5% HSA as required by the experiment.
4. Avoid repeated freeze-thaw cycles; it is recommended to prepare the required amount at once.

Storage & Stability

The lyophilized powder should be stored at -20°C with a shelf life of 1 year. After reconstitution, use within 1 week under 2-8°C conditions. For long-term storage, it is recommended to aliquot in a buffer containing a carrier protein or stabilizer and store at -20°C or -80°C for up to 3-6 months.

Shipping & Delivery

The product is shipped using blue ice (cold chain) to ensure temperature control throughout transit. The 20 µg specification is in stock and ships the next day. For other specifications, please contact us in advance to confirm the delivery time.

Compliance Statement

This product is intended for research use only and is not suitable for human clinical or diagnostic use. The user assumes all responsibility for any research involving patents or license restrictions conducted using this product. We are not liable for patent infringement or other violations arising from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please contact Yakeyin Company.