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1441-866沃特曼定量滤纸GR 41 20.3x25.4CM 100/PK Whatman
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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
, Wheaton). Homogenize using a loose pestle with 25 strokes. Transfer to 2 ml Eppendorf tubes and pellet the nuclei by centrifugation at 1500 g for 5 min. Transfer the supernatant to a new tube and clear it by spinning 15 min in a tabletop
in length) in 1X TBE. Load one of the lanes with 1 µg of 100-bp DNA stepladder. Migrate at 2-3 V/cm for 1-2 hours. Stain the gel for 30 minutes in a tray with 500 ml of H2O to which 20 µg of ethidium bromide has been added. Take a photograph of the gel
's-solution; 1 % SDS; 100 µg/ml heat-denatured sheared non- homologous DNA (Salmon sperm DNA or yeast tRNA) [100 ml: 25 ml 20 x SSC, 50 ml Formamide, 5 ml 100 x Denhardt's, 1 g SDS, 1 ml 10 mg/ml DNA] Hybridization-buffer: Prehybridization
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