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1002-055沃特曼定性滤纸GR 2 5.5CM 100/PK Whatman
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文献和实验James Hardwick angiotensin assay protocol
5. Spot 10 microliters of each supernatant onto a 2 x 2 cm phosphocellulose paper (Whatman p81). Wash the paper in 0.425% phosphoric acid (add 5 ml 85% phosphoric acid to 1 L H2O to make 0.425% phosphoric acid.) for 3 min (15 milliliters/piece
Protocol for Trichloroacetic Acid (TCA) Precipitation
sperm DNA, Ambion Cat # 9680 ; dilute to 1 mg/ml in water) Glass fiber filters to catch precipitate (e.g., Whatman GF/C filter circle; or Schleicher & Schuell #34 2.7-cm glass filter circles, Cat #10373828) Aqueous scintillation fluid
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. A small block made of polyvinyl chloride (2.5 x 2.5 x 1.5 cm) is attached to a flexible Teflon ribbon as shown. The block should hang ~2-3 mm above the plane of the holding block (also made of polyvinyl chloride). A piece of filter paper (i.e., Whatman 3MM
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