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- CAS-67153
- 2025年12月17日
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Flow cytometric analysis of Bcl-2 expression in HT-1080 cells using Bcl-2 antibody (Cat#CAS-67153, 1:2,000). Green, isotype control; red, Bcl-2.
Western blotting analysis using anti-Bcl 2 antibody (Cat#CAS-67153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#CAS-67153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#CAS-212, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#CAS-237).
Western blotting analysis using anti-Bcl-2 antibody (Cat#CAS-67153). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#CAS-67153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#CAS-212, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#CAS-727).
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文献和实验Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
MAbs (mAbs) are unique and versatile molecules that have found applications in research, in vitro and in vivo diagnostics, and the treatment of several diseases. Antibody technology has been revolutionized by the hybridoma technology for rnAb
Recombinant Monoclonal Antibodies
Recombinant antibody technology is a rapidly evolving field that enables the study and improvement of antibody properties by means of genetic engineering. Moreover, the functional expression of antibody fragments in Escherichia coli
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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