Annexin V-AbFluor™ 647 细胞凋亡检测试

Annexin V-AbFluor™ 647 细胞凋亡检测试剂盒（红色荧光）
Annexin V-AbFluor™ 647 Apoptosis Detection kit (Red Fluorescence)

货号：KTA0004 | 产品分类：核心产品 | 应用领域：细胞状态分析

产品概述

本试剂盒专为细胞凋亡/周期的高灵敏度检测设计，通过Annexin V-AbFluor™ 647与磷脂酰丝氨酸（PS）的特异性结合，结合碘化丙啶（PI）双染技术，实现凋亡早晚期细胞及死细胞的精准区分，适用于流式细胞术与荧光显微镜检测。

关键特性与优势

• 超强荧光性能：AbFluor™ 647相较Alexa Fluor 647、Cy5及DyLight™ 649，具备更高荧光强度与优异光稳定性
• 双通道检测：
  • Annexin V-AbFluor™ 647：Ex/Em = 650/665 nm（远红光）
  • PI：Ex/Em = 535/617 nm（兼容488/532/546 nm激光器）
• 广泛兼容性：适配主流流式细胞仪及荧光显微镜平台

试剂盒组分

• Annexin V 结合缓冲液(5x)
• Annexin V- AbFluor™ 647
• 碘化丙啶（PI）染色液

产品规格与价格

• 50 T | 价格：¥1,269 | 货期：现货（次日发货）
• 100 T | 价格：¥2,116 | 货期：现货（次日发货）

保存与运输

• 保存条件：4°C避光保存，有效期6个月（自发货日起计）
• 运输方式：冰袋（蓝冰）低温运输

重要声明

警告：本品仅用于研究用途，不适用于人类或临床诊断。使用前请确认实验方案不违反所在地区的专利或法规要求。

技术背景

细胞凋亡早期，磷脂酰丝氨酸（PS）外翻至细胞膜表面，Annexin V通过钙离子依赖性结合PS标记凋亡细胞，而PI仅穿透膜完整性受损的晚期凋亡/坏死细胞核，双染方案可实现对凋亡进程的阶段性判别。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Annexin V-AbFluor™ 647 Apoptosis Detection Kit (Red Fluorescence)
Annexin V-AbFluor™ 647 Apoptosis Detection Kit (Red Fluorescence)

Catalog No.: KTA0004 | Category: Core Products | Application: Cell Status Analysis

Product Overview

This kit is specifically designed for high-sensitivity detection of apoptosis/cell cycle. Through the specific binding of Annexin V-AbFluor™ 647 to phosphatidylserine (PS) and the dual-staining technique with propidium iodide (PI), it enables precise discrimination between early/late apoptotic cells and dead cells, suitable for flow cytometry and fluorescence microscopy.

Key Features and Advantages

• Superior Fluorescence Performance: AbFluor™ 647 exhibits higher fluorescence intensity and excellent photostability compared to Alexa Fluor 647, Cy5, and DyLight™ 649.
• Dual-Channel Detection:
  • Annexin V-AbFluor™ 647: Ex/Em = 650/665 nm (far-red)
  • PI: Ex/Em = 535/617 nm (compatible with 488/532/546 nm lasers)
• Broad Compatibility: Compatible with mainstream flow cytometers and fluorescence microscopy platforms.

Kit Components

• Annexin V Binding Buffer (5x)
• Annexin V-AbFluor™ 647
• Propidium Iodide (PI) Staining Solution

Specifications and Pricing

• 50 T | Price: ¥1,269 | Lead Time: In stock (shipped next day)
• 100 T | Price: ¥2,116 | Lead Time: In stock (shipped next day)

Storage and Shipping

• Storage Conditions: Store at 4°C protected from light. Valid for 6 months (from the date of shipment).
• Shipping Method: Shipped with ice packs (blue ice) under low-temperature conditions.

Important Notice

Warning: This product is for research use only and is not intended for human or clinical diagnosis. Please ensure that the experimental protocol does not violate any patent or regulatory requirements in your region before use.

Technical Background

During early apoptosis, phosphatidylserine (PS) is translocated to the outer leaflet of the cell membrane. Annexin V binds to PS in a calcium-dependent manner to label apoptotic cells, while PI only penetrates the nuclei of late apoptotic/necrotic cells with compromised membrane integrity. The dual-staining protocol enables stage-specific discrimination of the apoptosis process.

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Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakoyin Company.