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- BIO-RAD
- 12020043
- 2025年12月11日
- CJ-F
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
Conjugation Kit
- 标记物：
StarBright Violet 610
- 保质期：
自发货之日起 12 个月
- 目录编号：
TrailBlazer StarBright Violet 610 Label Kit
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
CJ-F
- 抗体名：
TrailBlazer StarBright Violet 610 Label Kit
- 规格：
1 X 100 µg Antibody Conjugation
TrailBlazerTM StarBrightTM Dye Label is the second part of a two-kit antibody labeling system. Together with the TrailBlazer Tag kit it will allow you to conjugate an antibody to superior StarBright Dyes using the innovative SpyTag and SpyCatcher technology. They provide the benefits of using StarBright Dyes in flow cytometry and western blots for your antibody of choice.
First, the SpyTag is attached to an antibody using the TrailBlazer Tag Kit. This antibody, now with a SpyTag, is then mixed with a StarBright Dye labeled SpyCatcher using the Label Kit. A spontaneous reaction occurs between the SpyTag and SpyCatcher resulting in an irreversible covalent isopeptide bond. The result is a highly stable StarBright Dye labeled antibody, which should be stored at +4°C in the dark, and when stored correctly will be stable for up to 12 months.
Batch specific Certificate of Analysis for TrailBlazer StarBright Dye Label Kits are available to be downloaded directly from the website.
In flow cytometry we suggest using 5µl of the undiluted labeled antibody to stain 106 cells in 100µl. However, we highly recommend that you titrate the antibody to determine the optimal amount of antibody for your specific experiment.
The TrailBlazer StarBright Dye Label Kits are available in different pack sizes and for a range of StarBright Dyes. Discover the full range and all the benefits of these TrailBlazer Tag and StarBright Dye Label Kits.
储存信息
The StarBright Dye labeled antibody should be stored undiluted at +4°C in the dark for up to 1 year.
应用
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Conjugation - Flow Cytometry | ✓ |
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
Instructions For Use
12020043
- Add the SpyTagged antibody product from TrailBlazer Tag Kit to the StarBright Dye-Catcher vial. Mix and incubate for 30 min at room temperature or overnight at +4°C.
- Add the sample to the Spin Column. Vortex briefly and mix on an end-over-end rotary mixer at room temperature for 60 min.
- Break open the bottom of the Spin Column and insert the column into the 2 ml amber collection tube. Slightly loosen the cap of the Spin Column and centrifuge at 1500 x g for 2 min. Retain the eluate, containing the label antibody.
- Prepare Quenching Reagent by adding 5 µl of ultrapure water.
- Add 4 µl of prepared Quenching Reagent and 45 µl of Storage Buffer to the labeled antibody. Vortex briefly to mix.
Antibodies are now labeled and ready to use. Store at +4°C in the dark for up to 1 year.
For full instructions for use please go to: bio-rad-antibodies.com/label-SB-kit-IFU.
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文献和实验. Note: SPW Wash Buffer Concentrate must be diluted with absolute ethanol before use. Refer to label on bottle for directions. 7) Discard liquid and centrifuge the empty column for 1 min at maximal speed ($13,000 x g) to dry the column
Applications The DC enriched cell population can be used for further isolation of DC subpopulations to high purity using flow sorting. 注意事项 Storage/Stability This product is stable until the expiry date stated on the label
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
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