- 询价
- Miltenyi Biotec
- 德国
- 130-114-278
- 2025年12月09日
- Flow cytometry
- human cell line
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD8b.2
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA865
- 标记物：
PE
- 适应物种：
mouse
- 保质期：
24个月
- 供应商：
Miltenyi biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry
- 浓度：
1:50
- 抗体英文名：
CD8b.2 Antibody, anti-mouse, REAfinity™
- 抗体名：
CD8b.2 Antibody, anti-mouse, REAfinity™
- 规格：
150 µg in 1 mL
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文献和实验suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Western Blotting with Horseradish Pe
protein of cell or tissue lysate to each well of a 0.75–1.0mm thick gel. For thicker gels (1.5mm thick), apply up to 25-40µg in each well. 3.Electrophorese until the bromophenol blue in the samples reaches the bottom of the gel. Turn off power supply
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