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CD42b Antibody, anti-human, PE

, REAfinity™, 30 tests in 60 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-123-959
  • 2025年12月09日
  • Flow cytometry, Functional assay
  • human cell line
  • human
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德国美天旎生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      CD42b

    • 亚型：

      recombinant human IgG1

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，4-8℃

    • 克隆性：

      REA185

    • 标记物：

      PE

    • 适应物种：

      human

    • 保质期：

      24个月

    • 供应商：

      Miltenyi Biotec

    • 宿主：

      human cell line

    • 应用范围：

      Flow cytometry, Functional assay

    • 浓度：

      1:50

    • 抗体英文名：

      CD42b Antibody, anti-human, REAfinity™

    • 抗体名：

      CD42b Antibody, anti-human, REAfinity™

    • 规格：

      30 tests in 60 µL

    Identification and enumeration of CD42b+ cells by flow cytometryClone REA185 recognizes CD42b, a 145 kDa single-pass type I membrane protein. Expression of CD42b is found on platelets and megakaryocytes. CD42b, the α chain, together with the β chain (CD42c), forms the heterodimer, glycoprotein Ib (GP Ib). The GP Ib receptor complex further includes association of the α and β subunits with platelet glycoprotein IX and glycoprotein V and this complex serves as the receptor for von Willebrand factor (VWF). The interaction of the GP Ib-IX-V complex to VWF initiates initial platelet adhesion to vascular subendothelium after vascular injury, platelet activation, thrombosis, and hemostasis. Other known ligands for GPIb–IX–V include α-thrombin, clotting factors XI/XIIa, and high-molecularweight kininogen. | Additional information: Clone REA185 displays negligible binding to Fc receptors. |

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    图标文献和实验
    该产品被引用文献
    Shrimpton, C. N. et al. (2002) Localization of the adhesion receptor glycoprotein Ib-IX-V complex to lipid rafts is required for platelet adhesion and activation. J. Exp. Med. 196: 1057. | Andrews, R. K. et al. (1997) Molecular mechanisms of platelet adhesion and activation. Int. J. Biochem. Cell Biol. 29 (1): 91–105. | Andrews, R. K. et al. (2003) Glycoprotein Ib-IX-V. Int. J. Biochem. Cell Biol. 35 (8): 1170–1174.
    相关实验

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      HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’.  Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide

    • Western Blotting with Horseradish Pe

      7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

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    CD42b Antibody, anti-human, PE, REAfinity™, 30 tests in 60 µL
    询价