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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD275 (B7-H2)
- 亚型:
mouse IgG1κ
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
MIH12
- 标记物:
PE
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
mouse
- 应用范围:
Flow cytometry
- 浓度:
1:11
- 靶点:
CD275 (B7-H2)
- 抗体英文名:
CD275 (B7-H2) Antibody, anti-human
- 抗体名:
CD275 (B7-H2) Antibody, anti-human
- 规格:
100 tests in 1 mL
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
. no.: 6 - 6201 - 004),內含 : 4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood) 1x CD3 Fab-Strep, human 1x Buffer CI (10x) 1x Biotin stock solution 1x Fab-TACS® Gravity Adapter Fab-TACS® Flow Restrictor, Pack of 5 1.1 用 ddH2O 将 10
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