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CD182 (CXCR2) Antibody, anti-h

uman, Biotin, REAfinity™, 30 tests in 300 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-100-922
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD182 (CXCR2)

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA208

    • 标记物

      Biotin

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry

    • 浓度

      1:11

    • 抗体英文名

      CD182 (CXCR2) Antibody, anti-human, REAfinity™

    • 抗体名

      CD182 (CXCR2) Antibody, anti-human, REAfinity™

    • 规格

      30 tests in 300 µL

    Identification and enumeration of CD182 (CXCR2)+ cells by flow cytometryClone REA208 recognizes CD182 (CXCR2), which belongs to the class of chemokine receptors specific for CXC chemokines. Chemokine receptors are G-coupled protein receptors (GPCR) with seven-transmembrane domains and consist of an α-subunit and a βγ-complex. In humans, interleukin 8 (IL-8) is one of most potent chemokine which binds to CXCR2. Expression of CD182 is found on several cell types including leukocytes, endothelial, and epithelial cells. CD182 plays a critical role in neutrophil homeostatis, angiogenesis, and in pathological conditions such as acute lung injury, lung diseases, and asthma. | Additional information: Clone REA208 displays negligible binding to Fc receptors. |

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    图标文献和实验
    该产品被引用文献
    Belperio, J. A. et al. (2005) CXCR2/CXCR2 ligand biology during lung transplant ischemia-reperfusion injury. J Immunol 175: 6931–6939. | Thatcher, T. H. et al. (2005) Role of CXCR2 in cigarette smoke-induced lung inflammation. Am. J. Physiol. Lung Cell Mol. Physiol. 289: L322–L328.
    相关实验
    • RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

      ) is then used to detect promoter specific transcripts (pRNAs) with the biotin linked pull-down assay (Figure 2) (comment 5);3.From the 200µl elute 30µl should be saved as input and the remaining 170µl from the extracted genomic DNA (Qiagen DNeasy) is mixed

    • CD3/CD28 做好 T 细胞激活与扩增

      细胞的状态,是否形成克隆团,或进行细胞计数,如果出现培养基变黄或细胞密度过高的情况,吹散细胞克隆团后调整细胞密度至0.5-1×10^6/ml,更换培养板和培养基; 4. 移除 CD3/CD28 Streptamers 终止刺激(该步根据实验需求可选做):直接向刺激后的细胞悬液中加入终浓度为 1mM 的 Biotin,室温孵育 30 分钟。收集并转移细胞至 15ml 离心管中,加入 10ml 培养基,300g 离心 6-10 分钟,弃上清,再次加入 10ml 培养基重复一遍洗涤步骤,至此,细胞已经可以用于

    • 用全血直接分选 CD3+ T 细胞

      . no.: 6 - 6201 - 004),內含 : 4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood) 1x CD3 Fab-Strep, human 1x Buffer CI (10x) 1x Biotin stock solution 1x Fab-TACS® Gravity Adapter Fab-TACS® Flow Restrictor, Pack of 5   1.1 用 ddH2O 将 10

    图标技术资料

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    文献支持
    CD182 (CXCR2) Antibody, anti-human, Biotin, REAfinity™, 30 tests in 300 µL
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