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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD182 (CXCR2)
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA208
- 标记物:
Biotin
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- 浓度:
1:11
- 抗体英文名:
CD182 (CXCR2) Antibody, anti-human, REAfinity™
- 抗体名:
CD182 (CXCR2) Antibody, anti-human, REAfinity™
- 规格:
30 tests in 300 µL
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文献和实验) is then used to detect promoter specific transcripts (pRNAs) with the biotin linked pull-down assay (Figure 2) (comment 5);3.From the 200µl elute 30µl should be saved as input and the remaining 170µl from the extracted genomic DNA (Qiagen™ DNeasy) is mixed
细胞的状态,是否形成克隆团,或进行细胞计数,如果出现培养基变黄或细胞密度过高的情况,吹散细胞克隆团后调整细胞密度至0.5-1×10^6/ml,更换培养板和培养基; 4. 移除 CD3/CD28 Streptamers 终止刺激(该步根据实验需求可选做):直接向刺激后的细胞悬液中加入终浓度为 1mM 的 Biotin,室温孵育 30 分钟。收集并转移细胞至 15ml 离心管中,加入 10ml 培养基,300g 离心 6-10 分钟,弃上清,再次加入 10ml 培养基重复一遍洗涤步骤,至此,细胞已经可以用于
. no.: 6 - 6201 - 004),內含 : 4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood) 1x CD3 Fab-Strep, human 1x Buffer CI (10x) 1x Biotin stock solution 1x Fab-TACS® Gravity Adapter Fab-TACS® Flow Restrictor, Pack of 5 1.1 用 ddH2O 将 10
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