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VGLUT1 Antibody (APC)抗体,orb149

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  • ¥8580
  • biorbyt已认证
  • orb149724
  • 英国
  • 2025年12月04日
  • IHC, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      VGLUT1 Antibody (APC)抗体

    • 抗体英文名

      VGLUT1 Antibody (APC)

    • 靶点

      VGLUT1

    • 浓度

      1 mg/ml

    • 应用范围

      IHC, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb149724

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      APC

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

    • 规格

      100 ug

    产品描述:Mouse monoclonal to VGlut1 (APC). VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate..

    别名:VGLUT1, Vesicular Glutamate Transporter 1, SLC17A7, BNPI, Solute Carrier Family 17 Member 7, Brain-specific Na(+)-dependent Inorganic Phosphate Cotransporter, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 7, Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7, VGluT1, VGLUT 1

    免疫原:Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

    克隆性:N28/9 (Formerly sold as S28-9)

    分子量:52kDa

    应用稀释比例:WB (1:1000)

    应用注释:1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:95.46mM Phosphate, 2.48mM MES and 2mM EDTA

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_446311.1

    Entrez:116638

    UniProt ID:Q62634

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.

    产品细节图片2
    Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X.

    产品细节图片3
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.

    产品细节图片4
    Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.

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